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Qualitative and quantitative detection method of aconitine phytotoxin

A quantitative detection method and aconitine technology are applied in the field of qualitative and quantitative detection of aconitine phytotoxins, can solve problems such as low detection limit, and achieve the effects of rapid response, low detection cost and high detection sensitivity

Active Publication Date: 2021-02-02
INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The relevant research on the detection of alkaloid phytotoxins by SERS technology disclosed in the prior art mainly focuses on isoquinoline derivatives and tropane derivatives, and the detection limit is low

Method used

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  • Qualitative and quantitative detection method of aconitine phytotoxin
  • Qualitative and quantitative detection method of aconitine phytotoxin
  • Qualitative and quantitative detection method of aconitine phytotoxin

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Embodiment 1

[0032] Such as figure 1 As shown, a qualitative and quantitative detection method of aconitine phytotoxins, comprising the steps:

[0033] (1) In a 96-well plate, mix 200 μL of 5-100 μg of aconitine, mesoaconitine, hypoaconitine, and mixed aconitine (mixture of aconitine, mesoaconitine, and hypoaconitine) / L standard solution with 50μL concentration of 1.5×10 -8 The silver nanoparticle sol of M is mixed evenly, and then adding 50 μ L of KI solution with a concentration of 1M to obtain a standard solution to be tested; the particle diameter of the silver nanoparticle sol is 100 nm;

[0034] (2) Place the above-mentioned 96 wells under the microscope, adjust the microscope so that the laser of the Raman spectrometer is focused on the liquid surface into a bright circular light plate for detection, scan the dark current before the test to reduce background interference, and use a wavelength of 785nm laser, the integration time is 2s, and the cumulative times are 5 times, the po...

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Abstract

The invention discloses a qualitative and quantitative detection method of aconitine phytotoxin. The method comprises the following steps: (1) uniformly mixing standard solutions with different concentrations of aconitine, aconitine, aconitine and mixed aconitine with silver nanoparticle sol respectively, and adding an inorganic salt solution to obtain a standard to-be-detected solution; (2) detecting the standard to-be-detected solution by using a Raman spectrometer, and recording the position and intensity of a characteristic peak; and (3) obtaining a to-be-detected sample solution from a to-be-detected sample according to the same method as step (1), detecting the to-be-detected sample solution by using the Raman spectrometer, recording the position and intensity of the characteristic peak, and carrying out qualitative and quantitative analysis on the variety of aconitine phytotoxins in the to-be-detected sample according to step (2). The method has the advantages of being simple and rapid in detection process, rapid in response, low in detection cost, high in detection sensitivity and the like, and can meet the actual requirements of plant toxin field detection.

Description

technical field [0001] The invention belongs to the technical field of Raman detection, and in particular relates to a qualitative and quantitative detection method of aconitine-like phytotoxins. Background technique [0002] Aconitine is a class of highly toxic plant toxins that widely exist in the environment. After entering the human body through food, skin contact, etc., it will cause severe poisoning reactions and even endanger life. Common detection methods for aconitine-like phytotoxins are high-performance liquid chromatography, liquid chromatography-tandem mass spectrometry, and gas chromatography-mass spectrometry. However, these methods require expensive equipment, complex operations, and long detection times, which are difficult to meet The actual needs of online monitoring and on-site emergency detection limit their wide application. Therefore, the development of cost-effective and rapid and sensitive detection methods for aconitine-like phytotoxins is crucial ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65G01N1/28
CPCG01N21/65G01N1/28
Inventor 刘国坤王维礼卢江龙顾家镭田中群
Owner INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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