Imaging method, and method, device and system for controlling sequencing reaction

Abstract

The invention discloses an imaging method, and a method, a device and a system for controlling a sequence determination reaction. The imaging method is applied to the optical detection system, whereinthe optical detection system comprises an imaging device and a bearing device; the bearing device comprises a temperature control device and a carrying table, wherein the imaging device comprises a lens module, the lens module comprises an optical axis, and the carrying table is used for bearing a sample, and the temperature control device is used for regulating the temperature of the sample. Theimaging method comprises the following steps: before the imaging device is used for carrying out image acquisition on a sample or when the imaging device is used for carrying out image acquisition onthe sample, the temperature control device is used for setting a range allowing temperature fluctuation of the sample, so that the position fluctuation range of the lens module along the optical axisis within a preset range. Thus, by means of the imaging method, the position fluctuation range of the lens module can be controlled within the preset range, and adverse effects on the imaging deviceduring image acquisition are reduced or avoided.

Description

technical field [0001] The invention relates to the field of optical detection, in particular to an imaging method, a method for controlling a sequence determination reaction, a control device, an optical detection system and a sequence determination system. Background technique [0002] Sequence determination, ie, sequencing, includes the determination of nucleic acid sequences. Sequencing platforms currently on the market include first-generation sequencing platforms, second-generation sequencing platforms, and third-generation sequencing platforms. From the perspective of functional control, the sequencing instrument includes a detection module, and the detection module is used to convert and / or collect the information changes generated by the biochemical reaction in the sequence determination to determine the sequence. The detection module generally includes an optical detection module, a current detection module and an acid-base (pH) detection module. The sequencing p...

AI Technical Summary

Problems solved by technology

[0003] In the process of collecting optical signals, the lens module of the optical detection module will have unexpected position fluctuations, which will have an adverse effect on the acquisition of optical signals

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