Coded illumination real-time focus scanning imaging device and method

Abstract

The invention discloses a coded illumination real-time focusing and scanning imaging device, which includes an LED array, an object stage, an objective lens, a liquid lens, a lens sleeve and a camera, wherein the LED array is located on one side of the object stage, and the The objective lens, the liquid lens, the lens tube and the camera are located on the other side of the stage, the stage is used to place samples, and the LED array is used to emit bright field light or encode The light, the bright field light or the coded light respectively passes through the sample and then passes through the objective lens, the liquid lens and the lens sleeve to reach the camera to take bright field images or coded images through the camera. Light illuminates the image. The invention also discloses a real-time focusing and scanning imaging method with coded illumination, which is carried out by using the above-mentioned device. The invention can realize rapid and accurate focusing at low cost.

Description

technical field [0001] The invention relates to the field of imaging technology, in particular to a coded illumination real-time focus scanning imaging device and method. Background technique [0002] In 2017, Philips' wsi (whole slide imaging) system passed the FDA (Food and Drug Administration, US Food and Drug Administration) certification for clinical diagnosis, marking the feasibility of digital pathology in clinical medicine . Whole-slice imaging digitizes pathological slides and stores them in a computer for easy storage and reference. The advancement of this technology can greatly promote the development of digital pathology. In order to obtain high-resolution images, an objective lens with a high numerical aperture is required during the whole-slice image acquisition process, so that the depth of field of the system is very small. Correct focusing to keep the system within the depth of field is an important factor for obtaining clear whole-slice images. [0003] T...

AI Technical Summary

Problems solved by technology

[0003] The traditional focusing method is based on the z-stack method, taking a large number of pictures within the z-axis range and selecting the one with the highest image quality as the quasi-focus image, but this method requires a lot of time expenditure

In addition, there is also a focusing method based on the focus map. In a part of the sample field of view, the in-focus position is obtained through the stack, and the in-focus position of the entire field of view of the sample is obtained by interpolation. However, this method requires additional time to obtain the focus map.

In addition, there is a way to fix the focus based on reflection, and determine the defocus amount by detecting the position of the laser reflected by the sample, but this method requires additional devices and increases the cost

[0004] The additional time expenditure or additional hardware equipment requirements in the above methods have affected the promotion of wsi in clinical applications

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