An organ chip and its application

Abstract

The invention discloses an organ chip and its application. The chip includes: a multi-cell co-culture structure and a concentration gradient structure, and the concentration gradient structure is used to provide liquid with a preset concentration gradient to the multi-cell co-culture structure; The multi-cell co-culture structure includes a plurality of cell culture units and liquid collection chambers corresponding to the plurality of cell culture units, and the liquid collection chamber is used to collect the liquid discharged from the corresponding cell culture units; the concentration gradient structure It includes a first channel and a second channel arranged in parallel, a plurality of first sub-channels arranged at intervals are drawn from the first channel, and the outlets of the plurality of first sub-channels are connected with the second sub-channels, the The second channel leads out a second sub-channel between two adjacent first sub-channels, and the outlet of the second sub-channel is connected with the plurality of cell culture units. The invention can realize the co-cultivation of liver cells to simulate the microenvironment in vivo, so that the results of drug screening are more accurate and reliable.

Description

technical field [0001] The invention relates to the technical field of microfluidic organ chips, in particular to an organ chip and applications thereof. Background technique [0002] Organ chip is short for microfluidic organ chip. It is a multi-channel, three-dimensional cell culture device containing continuous perfusion chambers. It consists of two parts. , and the other part includes the microenvironment composed of cells, secretions and physical forces around the corresponding organs. With the development of modern medicine and modern pharmacology, more and more drug screening models are prepared using animal tissues and organs, such as in vitro blood vessel experiments, cardiac perfusion experiments, tissue culture experiments and other methods. However, this model is small in scale, low in efficiency, limited in the effect of reacting drugs, requires a large amount of samples, and is not easy to achieve multiple screening for one drug. In addition, the high technica...

AI Technical Summary

Problems solved by technology

However, this type of model is small in scale, low in efficiency, limited in response to drugs, requires a large amount of samples, and is not easy to achieve multiple screening of one drug. In addition, the high technical requirements for manual operation are also one of the main reasons affecting its application in drug screening.

[0003] At present, after nearly 10 years of development of organ chips, various human organ chips have been developed, such as: liver chip, lung chip, intestine chip, kidney chip, blood vessel chip, heart chip, etc., but most of the chips are a single type of cell Two-dimensional culture still has a large gap with the in vivo microenvironment of natural organs. It is difficult to obtain similar or identical results to in vivo tests for drug screening, and most of the cells in the chip are closed culture, which limits the ability of the drug screening process. Analysis and detection of cells and proteins

Images