Mouse myelin depletion model feed and preparation method and application thereof

A kind of myelin sheath and mouse technology, applied in the field of mouse demyelination model feed and preparation thereof

Pending Publication Date: 2021-02-26
常州鼠一鼠二生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Aiming at the deficiencies of the above-mentioned prior art, the present invention provides a method for preparing a mouse demyelinating model feed, the purpose is to solve the problem that cannot be completely exempted due to the limitation of nerve tissue sampling in the research of multiple sclerosis. Technical issues in obtaining information on different aspects of the disease

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  • Mouse myelin depletion model feed and preparation method and application thereof
  • Mouse myelin depletion model feed and preparation method and application thereof
  • Mouse myelin depletion model feed and preparation method and application thereof

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Embodiment 1

[0022] The preparation method of the mouse demyelination model feed provided by the present embodiment, the specific technical scheme is as follows:

[0023] Such as figure 1 Shown, the preparation method of mouse demyelination model feed comprises the steps:

[0024] S1, accurately weigh the raw materials shown in Table 1, by mass ratio, 49.9% sucrose, 0.3% methionine, 3.49% complex minerals, 1% complex vitamins, 19.96% casein, 14.97% cornstarch, 4.99% cellulose , 0.20% choline bitartrate and 0.2% dicyclohexanone oxalyldihydrazone are mixed to obtain a mixed powder;

[0025] S2, adding corn oil with a mass ratio of 4.99% to the mixed powder in step S1 for stirring to obtain an oil mixture;

[0026] S3, the oil mixture in step S2 is placed in a cooling bucket to cool down, after cooling down to room temperature, adding water to stir and mix, and granulating through a granulator to obtain soft pellet feed, the particle diameter of which is 10-15mm;

[0027] S4, drying the so...

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Abstract

The invention belongs to the technical field of experimental animals, and particularly relates to a preparation method of a mouse myelin depletion model feed. The preparation method comprises the following steps of: accurately weighing a basal feed and 0.2% by mass of bicyclohexahexanone oxalyl dihydrazone, mixing, adding corn oil, stirring, cooling in a cooling barrel, adding water, stirring andmixing, and granulating by a granulator to obtain a soft granulated feed; and drying at low temperature to obtain the mouse myelin depletion model feed. The invention also provides a mouse myelin depletion model feed and application thereof. The mouse myelin depletion model successfully copies the animal model similar to the pathogenesis of human myelin depletion, and opens up a new way for the research of the pathogenesis of a plurality of sclerosis diseases. Besides, the mouse myelin depletion model can be obtained by feeding the mouse with a simple feed without using a gavage means, which conforms to the animal ethics, thereby providing an animal model research basis for screening drugs for multiple sclerosis diseases.

Description

technical field [0001] The invention belongs to the technical field of experimental animals, and in particular relates to a mouse demyelination model feed and a preparation method and application thereof. Background technique [0002] The central nervous system (CNS) includes the brain and spinal cord. During the onset of multiple sclerosis (MS), peripheral immune cells enter the central nervous system through the blood-brain barrier with increased permeability, forming many inflammatory infiltrating lesions in the central nervous system, leading to demyelination and axonal damage. Myelin sheath is a multi-layered membranous structure wrapped around the surface of the axon, composed of lipids and proteins, which can not only protect the axon but also insulate the conduction of nerve impulses. At present, the pathogenesis of MS is still unclear, and its pathological changes are mainly demyelination and remyelination disorders. MS is characterized by recurrent attacks and pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/50A23K20/163A23K20/142A23K20/20A23K20/174A23K20/147A23K20/105A23K20/158A01K67/02
CPCA23K50/50A23K20/163A23K20/142A23K20/30A23K20/20A23K20/174A23K20/147A23K20/105A23K20/158A01K67/02A01K2267/03A01K2227/105
Inventor 姜海善
Owner 常州鼠一鼠二生物科技有限公司
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