Emission detection method

Abstract

The invention relates to an emission light detection method. The emission light detection method comprises: a plurality of excitation light sources irradiating the substance to be detected according to a preset method, so as to excite the substance to be detected, so that the emission light detection device detects the emission light generated by the substance to be detected. ; The substance to be detected contains a fluorescent group, which is used to generate emission light under the excitation of the excitation light; the emission light detection device is arranged on the optical path of the emission light, and captures and detects the emission light; in the same detection process, each excitation light source according to The preset M test cycles emit excitation light, and each excitation light sequentially completes the excitation of M test cycles, and the emission light detection device obtains the corresponding light emitted by each excitation light within the preset M test cycles. emit light. The emitted light detection method provided by the embodiment of the present invention has multiple excitation light sources, which can be detected simultaneously, and the emitted light detection device continuously acquires the intensity of emitted light, so that the experiment efficiency is high, convenient and efficient.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to an emission light detection method. Background technique [0002] In recent years, fluorescence analysis technology has been widely used in scientific research and production. Fluorescence analysis technology refers to that the excitation light device of a certain wavelength is irradiated on the substance to be detected, and the atoms or molecules in the substance to be detected absorb the energy of the excitation light, and the produce transitions. The atoms or molecules after the transition are extremely unstable and will return to the stable state of the ground state at any time, and energy will be released when the transition state returns to the ground state, that is, molecules and atoms will produce fluorescence. The fluorescence information is captured and analyzed by the spectrometer, so as to obtain what substance is contained in the substance to be detected. This t...

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Problems solved by technology

[0004] For this reason, the present invention provides a kind of emission light detection method, in order to solve the problem of low experimental efficiency in the prior art

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