Preparation method and application of double-drug combined intelligent antibacterial hydrogel

A hydrogel and intelligent technology, which is applied in the direction of pharmaceutical formulations, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc., can solve the problem of inability to change the kinetic characteristics and tissue distribution characteristics of drugs, and the limitations of clinical applications , drug resistance and other issues, to achieve the effect of non-toxic and harmless biocompatibility, excellent antibacterial effect, and convenient use

Active Publication Date: 2021-03-12
WUHAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the resistance of many clinical common pathogens to this type of drug, its clinical application has been limited to a certain extent.
[0005] The simple drug superposition used in clinical practice cannot change the inherent in vivo kinetic characteristics and tissue distribution characteristics of drugs, while the drug efficacy and side effects are closely related to their absorption, distribution and uptake in vivo

Method used

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  • Preparation method and application of double-drug combined intelligent antibacterial hydrogel
  • Preparation method and application of double-drug combined intelligent antibacterial hydrogel
  • Preparation method and application of double-drug combined intelligent antibacterial hydrogel

Examples

Experimental program
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Effect test

Embodiment 1

[0035] Provide a kind of preparation method of double-drug combined intelligent antibacterial hydrogel, comprising the following steps:

[0036] (1) Dissolve 1 g of dextran (Dex, molecular weight 40 kDa) in deionized water, configure it as a 0.1 g / mL Dex solution, stir it magnetically, slowly add 8 mL of sodium periodate (0.5 M, 107 mg / mL) solution dropwise into In the Dex solution, stirred at room temperature and protected from light for 4 hours, then added 2.4 mL of ethylene glycol to terminate the reaction, continued to stir and react at room temperature and protected from light for 2 hours, then placed the mixed solution in a dialysis bag, and deionized water Carry out dialysis (MwCO: 3500), after dialysis for 48 hours, ODex is obtained after freeze-drying for 48 hours. The degree of oxidation was measured by hydroxylamine hydrochloride titration method as follows: weigh ODex, dissolve it in deionized water with an appropriate amount of hydroxylamine hydrochloride solution...

Embodiment 2

[0056] Provide a kind of preparation method of double-drug combined intelligent antibacterial hydrogel, comprising the following steps:

[0057] (1) Dissolve 1g of Dex (Dex, molecular weight 40kDa) in deionized water, configure it as a 0.1g / mL Dex solution, stir it magnetically, and slowly add 8mL of sodium periodate (0.5M, 107mg / mL) solution dropwise to Dex solution, stirred at room temperature and protected from light for 4 hours, then added 2.4 mL of ethylene glycol to terminate the reaction, continued to stir and react at room temperature and protected from light for 2 hours, and then placed the mixed solution in a dialysis bag and carried out with deionized water. Dialysis (MwCO: 3500), after dialysis for 48 hours, ODex was obtained after freeze-drying for 48 hours. The degree of oxidation of the prepared ODex is 28.8%.

[0058] (2) Weigh 1g of lyophilized ODex and dissolve it in deionized water to make a 0.1g / mL ODex solution, then dissolve an appropriate amount of sulf...

Embodiment 3

[0076] Provide a kind of preparation method of double-drug combined intelligent antibacterial hydrogel, comprising the following steps:

[0077](1) Dissolve 1g of Dex (Dex, molecular weight 40kDa) in deionized water, configure it as a 0.1g / mL Dex solution, stir it magnetically, and slowly add 8mL of sodium periodate (0.5M, 107mg / mL) solution dropwise to Dex solution, stirred at room temperature and protected from light for 4 hours, then added 2.4 mL of ethylene glycol to terminate the reaction, continued to stir and react at room temperature and protected from light for 2 hours, and then placed the mixed solution in a dialysis bag and carried out with deionized water. Dialysis (MwCO: 3500), after dialysis for 48 hours, ODex was obtained after freeze-drying for 48 hours. The degree of oxidation of the prepared ODex is 28.8%.

[0078] Weigh 1g of lyophilized ODex and dissolve it in deionized water to make a 0.1g / mL ODex solution, then dissolve an appropriate amount of sulfadiaz...

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Abstract

The invention discloses a preparation method and application of double-drug combined intelligent antibacterial hydrogel. The preparation process of the hydrogel comprises the following steps: oxidizing dextran (Dex) by adopting a sodium periodate oxidation method, breaking part of chains in dextran to form furfural, then reacting primary amino in sulfadiazine with part of aldehyde groups in furfural to generate pH sensitive imine bonds, and reacting and cross-linking residual aldehyde groups in furfural with tobramycin in water, and carrying out self-assembly to form the hydrogel. The hydrogelhas a pH sensitive characteristic, can effectively release drugs in an inflammatory subacid environment, realizes on-demand release of the drugs, avoids excessive use of antibiotics, reduces toxic and side effects of the drugs, simultaneously loads two bactericidal drugs sulfadiazine and tobramycin, kills bacteria through different mechanisms, improves an antibacterial effect, and has an excellent antibacterial effect.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a preparation method and application of a dual-medicine combined intelligent antibacterial hydrogel. Background technique [0002] The treatment of bacterial infection is one of the most challenging topics in the field of biomedicine, and bacterial infection still poses a serious threat to human life. Studies have shown that patients with severe body trauma are extremely vulnerable to external and endogenous organ microbial invasion and infection due to damaged physiological protective barriers on the body surface, decreased immune function, and large accumulation of necrotic tissue. The whole course of the disease causes great suffering to the patient. Therefore, it is of great significance to improve the diagnosis and treatment of trauma infection to reduce the suffering and mortality of patients. [0003] Tobramycin (TOB), is a rapid fungicide. Also known as Anxin, Anti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/63A61K31/702A61K9/06A61K47/61A61P31/04
CPCA61K31/63A61K31/702A61K9/06A61K9/0019A61K9/0014A61K47/61A61P31/04A61K2300/00Y02A50/30
Inventor 郑化张梦瑶雷孟珩陈钢李单
Owner WUHAN UNIV OF TECH
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