Extraction method of phlorotannin stock solution

A technology of brown algae polyphenol stock solution and extraction method, which is applied in the direction of medical raw materials derived from algae, pharmaceutical formulas, plant raw materials, etc., can solve the problems of limiting the application prospects of brown algae polyphenols, difficulty in realizing industrial production, and long extraction cycle, etc., to achieve easy Industrialized production, convenient operation and excellent product quality

Inactive Publication Date: 2021-03-12
SHANDONG JIEJING GROUP CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the solvent extraction method has the defect of long extraction period; although the purity of the brown algae polyphenols extracted by the supercritical extraction method is relatively high, it has the disadvantages of long extraction period and low extraction amo...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A method for extracting brown algae polyphenol stock solution, the method is as follows:

[0030] (1) Wash and soak fresh brown algae for 2-3 hours, chop and dehydrate until the water content is lower than 80%, and set aside;

[0031] (2) Add the dehydrated brown algae in step (1) to ethanol for extraction, the ethanol concentration (volume concentration) is 40%, the ratio of solid to liquid is 1:5 (g / mL), the extraction time is 3-4h, filter, and remove the residue ;The temperature control range during the extraction process is 18-20°C;

[0032] (3) Centrifuge the extract obtained in step (2) (centrifugation speed is 6000 rpm), and the centrifugation time is 5-7 hours, and take the supernatant; the temperature control range during the centrifugation temperature process is below 20°C;

[0033] (4) Evaporating and concentrating the supernatant obtained in step (3) at a temperature of 18-20°C. Be concentrated to the ethanol concentration in feed liquid and be 20%;

[00...

Embodiment 2

[0038] A method for extracting brown algae polyphenol stock solution, the method is as follows:

[0039] (1) Wash and soak fresh brown algae for 2-3 hours, chop and dehydrate until the water content is lower than 80%, and set aside;

[0040] (2) adding the dehydrated brown algae in step (1) into ethanol for extraction. The ethanol concentration (volume concentration) is 60%, the ratio of solid to liquid is 1:5 (g / mL), the extraction time is 3-4h, and the residue is removed by filtration; the temperature control range during the extraction process is 18-20°C

[0041] (3) Centrifuge the extract obtained in step (2) (centrifugation speed is 6000 rpm), and the centrifugation time is 5-7 hours, and take the supernatant; the temperature control range during the centrifugation temperature is below 20°C.

[0042] (4) Evaporating and concentrating the supernatant obtained in step (3) at a temperature of 18-20°C. Concentrate until the concentration of ethanol in the feed liquid is 20-...

Embodiment 3

[0047] A method for extracting brown algae polyphenol stock solution, the method is as follows:

[0048] (1) Wash and soak fresh brown algae for 2-3 hours, chop and dehydrate until the water content is lower than 80%, and set aside;

[0049] (2) adding the dehydrated brown algae in step (1) into ethanol for extraction. The concentration of ethanol (volume concentration) is 80%, the ratio of solid to liquid is 1:5 (g / mL), the extraction time is 3-4h, filter and remove the residue; the temperature control range during the extraction process is 18-20°C

[0050] (3) Centrifuge the extract obtained in step (2) (centrifugation speed is 6000 rpm), the centrifugation time is 5-7 hours, and take the supernatant; the temperature control range during the centrifugation temperature is 18-20°C

[0051] (4) Evaporating and concentrating the supernatant obtained in step (3) at a temperature of 18-20°C until the concentration of ethanol in the feed liquid is 20-40%;

[0052] (5) Centrifuge ...

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PUM

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Abstract

The invention discloses an extraction method of a phlorotannin stock solution. The extraction method comprises the following steps: cleaning, soaking, chopping and dehydrating fresh brown algae in sequence; adding the dehydrated brown algae into an extracting agent for extraction, and then removing residues through a filtering process to obtain an extracting solution; centrifuging the extract, taking supernatant, carrying out low-temperature evaporation and concentration, centrifuging the concentrated feed liquid, taking supernatant, carrying out low-temperature evaporation and concentration again, concentrating until the concentration of the extractant in the feed liquid is below 5%, then carrying out low-temperature crystallization, centrifuging the concentrated liquid subjected to low-temperature crystallization again, and thus obtaining a supernatant, namely a phlorotannin stock solution; wherein the procedures of extraction, centrifugation and low-temperature evaporation and concentration are all carried out in an environment with the temperature of 18-20 DEG C. The scheme has the beneficial effects of simple extraction process, short period, low cost, high product quality andeasiness in industrial production.

Description

technical field [0001] The invention relates to the technical field of extracting brown algae active substances, in particular to a method for extracting brown algae polyphenol stock solution. Background technique [0002] Seaweed is algae that grows in the sea. It is mainly composed of four major categories: cyanobacteria, green algae, red algae and brown algae. Among them, brown algae is a relatively advanced group of algae, with about 250 genera and more than 1,500 species. Brown algae has the characteristics of fast growth, large algae body, wide distribution, etc., and has huge potential economic value. It occupies a prominent position in the economic resources of various coastal varieties in my country, and is one of the three major economic seaweeds in my country. Brown algae polyphenols are secondary metabolites of brown algae. They are a type of natural product with a unique structure. Anti-drought, anti-low temperature and other biologically active functions. The...

Claims

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Application Information

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IPC IPC(8): A61K36/03
CPCA61K36/03A61K2236/13A61K2236/15A61K2236/17A61K2236/333A61K2236/51A61K2236/53
Inventor 林成彬韩晓阳范斌赵丽薄宁姜玉敏
Owner SHANDONG JIEJING GROUP CORP
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