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DNA methylation marker for Psoriatic Arthritis, diagnostic reagent and application thereof

A methylation and psoriasis technology, applied in the field of medical molecular biology detection, can solve problems such as early diagnosis of patients without PsA

Active Publication Date: 2021-03-16
THE SECOND XIANGYA HOSPITAL OF CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current diagnosis is mainly based on the patient's medical history, clinical manifestations, and joint X-ray changes, and there are currently no biological diagnostic markers for early diagnosis of PsA patients

Method used

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  • DNA methylation marker for Psoriatic Arthritis, diagnostic reagent and application thereof
  • DNA methylation marker for Psoriatic Arthritis, diagnostic reagent and application thereof
  • DNA methylation marker for Psoriatic Arthritis, diagnostic reagent and application thereof

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Experimental program
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Embodiment

[0068] 1. DNA methylation CpG site screening

[0069] Whole blood samples from 25 cases of psoriatic arthritis and 19 cases of healthy controls were detected by whole blood genome 850k methylation chip, and a batch of DNA methylation with differential expression between psoriatic arthritis and healthy controls was preliminarily screened CpG sites.

[0070] 2. Identification of DNA methylation CpG sites

[0071] Through the design of primers, PCR amplification of target gene bands, pyrosequencing and other experiments. Finally, differentially expressed DNA methylated CpG sites were screened and identified for the diagnosis of PsA.

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Abstract

The invention discloses a DNA methylation marker for Psoriatic Arthritis (PsA), a diagnostic reagent and application thereof. The DNA methylation marker comprises one or more of six methylation sites:chr14: 38061326, chr14: 38061320, chr9: 128585454, chr6: 37225002, chr3: 101901234 and chr6: 97369501. The specific DNA methylation sites are used as molecular markers for the first time to assist inscreening patients with Psoriatic Arthritis and Rheumatoid Arthritis (RA), and a new way and mode are opened up for diagnosis in the field. The DNA methylation sites are detected by using whole bloodof the patients, the purpose of early diagnosis of PsA is achieved, the required blood samples are few, the DNA methylation marker is convenient and easy to carry out, and the DNA methylation markerhas a good application prospect.

Description

technical field [0001] The invention belongs to the technical field of medical molecular biology detection, and in particular relates to diagnostic markers, diagnostic reagents and applications of psoriatic arthritis. Background technique [0002] Psoriatic arthritis (PsA) is an inflammatory joint disease associated with psoriasis that has a psoriatic rash and causes pain, swelling, tenderness, stiffness, and movement disturbances in the joints and surrounding soft tissues. Some patients may have sacroiliitis and / or spondylitis, the course of the disease is protracted, easy to relapse, and may have ankylosis in the late stage, resulting in disability. The peak age of onset of PsA is 35-45 years old. About 70% of PsA patients have psoriatic skin lesions before joint inflammation, and about 15% of patients have skin lesions and joint inflammation at the same time. Early PsA refers to the absence of pain symptoms within 1-2 years after the patient is diagnosed. Therefore, it i...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883C12Q2600/158
Inventor 陆前进赵明苏玉文邓敏吴海竞
Owner THE SECOND XIANGYA HOSPITAL OF CENT SOUTH UNIV