Efficient cell culture dish perfusion device

Abstract

The invention relates to the technical field of biological fermentation, and discloses an efficient cell culture dish perfusion device. The device comprises a standard dish; a sealing cover is arranged above the standard dish; a liquid inlet pipe is arranged on the right side of the sealing cover; a liquid outlet pipe is arranged on the left side of the sealing cover; a temperature controller is arranged above the sealing cover; a flow valve is arranged on the upper portion of the right side of the temperature controller; an air inlet pipe is movably connected to the upper portion of the flowvalve; a parallel connection unit is movably connected to the lower end of the air inlet pipe; an aeration plate is connected to the lower portion of the parallel connection unit through a pipeline; an exhaust pump is arranged in the middle of the sealing cover; and a return air wheel is connected to the upper portion of the exhaust pump through a pipeline. According to the efficient cell culturedish perfusion device, exhaust is controlled by the exhaust pump, return air is intermittently pumped out through the return air wheel and emerges from the interior of an interception fiber pipe to the exterior through an aeration head, and cells and products intercepted outside the interception fiber pipe are repeatedly cleaned, so that the interception fiber pipe is effectively prevented from being blocked, the culture density is improved, and a basis is provided for increasing the perfusion flow.

Description

technical field [0001] The invention relates to the technical field of biological fermentation, in particular to a high-efficiency cell culture dish perfusion device. Background technique [0002] The perfusion culture process is a commonly used fermentation process. Stirring cell culture systems are mostly used, and tube systems can also be used. During the cell culture process, the culture medium is continuously perfused into the reactor and flows out at the same flow rate, so that the production process does not need to open the reactor, providing a relatively constant culture environment, so the fermentation density is high and the risk of contamination is low. However, this kind of reactor is generally used in industrial production, with good sealing and large capacity. When used in the laboratory, it is difficult to take samples for detection, and the experiment period is long and the cost is high. Poor shutoff performance results in low cell density, low cell growth ...

AI Technical Summary

Problems solved by technology

However, this kind of reactor is generally used in industrial production, with good sealing and large capacity. When used in the laboratory, it is difficult to take samples for detection, and the experiment period is long and the cost is high. Poor shut-off performance, resulting in low cell density, low cell growth efficiency, and large experimental errors

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