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Small peptide with function of inhibiting liver cancer metastasis and preparation method and application of small peptide

A technology of small molecule peptides and nucleotides, applied in the field of small peptides with the function of inhibiting the metastasis of liver cancer and the field of preparation thereof, can solve problems such as no high-value applications, and achieve the inhibition of the migration ability of liver cancer cells, and the inhibition of the invasion and metastasis of liver cancer cells. Effect

Active Publication Date: 2021-03-26
SHENZHEN HOSPITAL OF SOUTHERN MEDICAL UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

pri-miR-122 is stable and abundant in normal hepatocytes but does not have any high-value applications

Method used

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  • Small peptide with function of inhibiting liver cancer metastasis and preparation method and application of small peptide
  • Small peptide with function of inhibiting liver cancer metastasis and preparation method and application of small peptide
  • Small peptide with function of inhibiting liver cancer metastasis and preparation method and application of small peptide

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Embodiment Construction

[0072] In order to make the object, technical solution and technical effect of the present invention clearer, the present invention will be further described in detail below in conjunction with specific embodiments. It should be understood that the specific implementations described in this specification are only for explaining the present invention, not for limiting the present invention.

[0073] The experimental materials and reagents used, unless otherwise specified, are conventional consumables and reagents that can be obtained from commercial channels.

[0074] Translation validation assay of pri-miR-122

[0075] Separation principle of polyribosomes in cells by sucrose density gradient centrifugation:

[0076] If the number of ribosomes bound to an RNA is more, the RNA sedimentation rate will be faster during gradient centrifugation. Therefore, RNA bound to different numbers of ribosomes can be separated in the solution by centrifugation. Using qPCR to detect the cont...

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Abstract

The invention discloses a small peptide with a function of inhibiting liver cancer metastasis as well as a preparation method and application of the small peptide. The small peptide has amino acid sequences shown as SEQ ID NO.1-4 or a sequence which is obtained by substituting, deleting and / or adding one or more amino acids to any one of the amino acid sequences shown as SEQ ID NO.1-4 and / or has afunction of inhibiting liver cancer metastasis after terminal modification. The invention further provides the preparation method of the small peptide, and an amino acid site mediating rapid degradation of the small peptide is obtained. The small peptide is found to have the function of inhibiting invasion and metastasis of liver cancer cells, is strong in capacity of inhibiting migration of theliver cancer cells, and has the application potential of developing novel liver cancer cell metastasis resisting drugs or other preparations.

Description

technical field [0001] The invention belongs to the field of biologically active peptides, and in particular relates to small peptides capable of inhibiting liver cancer metastasis, a preparation method and application thereof. Background technique [0002] Hepatocellular carcinoma (hepatocellular carcinoma for short) is one of the most common malignant tumors with high morbidity and mortality worldwide. It causes more than 600,000 deaths every year and seriously endangers people's life and health. Most liver cancer patients have already entered the advanced stage of liver cancer when they are diagnosed, and cancer cell metastasis may have occurred. Applicable treatment methods are limited and the prognosis is poor. For patients with early liver cancer, surgical resection is currently the most important treatment, but its prognosis is poor, and recurrence and metastasis are easy to occur after surgery, resulting in the death of liver cancer patients. [0003] miR-122 is a l...

Claims

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Application Information

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IPC IPC(8): C07K14/47C12N15/11C12N5/10A61K38/16A61P35/00A61P35/04
CPCC07K14/4703A61P35/00A61P35/04A61K38/00
Inventor 曾春贤黄宁
Owner SHENZHEN HOSPITAL OF SOUTHERN MEDICAL UNIV
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