A Chinese herbal medicine microecological compound preparation for preventing and treating neonatal piglet enteritis and its preparation method and application

A compound preparation and newborn piglet technology, which is applied in the field of veterinary traditional Chinese medicine and feed additives, can solve the problems of piglets' own immunity decline and pathogenic bacteria resistance to drugs, so as to improve the body's immunity, prevent piglets from diarrhea, and improve daily growth rate. heavy effect

Active Publication Date: 2022-05-24
JIANGXI AGRICULTURAL UNIVERSITY +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past, the control measures for piglet diarrhea were mainly the use of antibiotics, but long-term use of antibiotics will lead to drug resistance of pathogenic bacteria, resulting in the decline of piglets' own immunity

Method used

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  • A Chinese herbal medicine microecological compound preparation for preventing and treating neonatal piglet enteritis and its preparation method and application
  • A Chinese herbal medicine microecological compound preparation for preventing and treating neonatal piglet enteritis and its preparation method and application
  • A Chinese herbal medicine microecological compound preparation for preventing and treating neonatal piglet enteritis and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Screening of Lactobacillus plantarum R23 and Lactobacillus amylovora K2

[0028] 1. Preliminary screening

[0029] Based on the results of gut microbiota analysis of healthy / diarrheal piglets and the birth canal of healthy / endometritis sows, Lactobacillus species, the dominant bacteria in the birth canal of healthy sows and the gut of healthy piglets, were isolated. 10 healthy sow birth canal samples and 10 healthy piglet fecal samples were collected. The samples were diluted to appropriate gradients with normal saline, and spread on MRS medium. 8 strains of lactic acid bacteria were obtained by culture, which were temporarily named as K1, K2, and 12.1. , 12.2, H1, R12, R22, R23.

[0030] Preparation method of MRS liquid medium: peptone 10.0g, beef extract powder 5.0g, yeast extract powder 4.0g, triammonium citrate 2.0g, glucose 20.0g, Tween 80 1.0mL, sodium acetate 5.0g, dipotassium hydrogen phosphate 2.0g, magnesium sulfate 0.2g, manganese sulfate 0.05g, p...

Embodiment 2

[0045] Example 2 Preparation of Chinese herbal medicine microecological compound preparation

[0046] 1) Mix 40 parts of Astragalus root, 30 parts of Shanxiang round leaf, 30 parts of dandelion, 25 parts of Daqingye, 18 parts of Artemisia argyi, and five kinds of Chinese herbal medicines, add 3 times the volume of water and boil 3 times, 50min each time, combine and fry 3 times The liquid is boiled and concentrated to a compound Chinese herbal medicine with a crude drug content of 0.5g / mL.

[0047] 2) The compound Chinese herbal medicine prepared in step 1) is added to MRS broth to prepare a culture solution, the pH value is adjusted to 7.0, sterilized by high pressure steam at 115° C. for 30 minutes, and after cooling to room temperature, Lactobacillus plantarum R23 and Lactobacillus amylovora K2 was inserted into the above-mentioned culture medium with an inoculum of 3%, and fermented at 37° C. for 72 hours to prepare a Chinese herbal medicine microecological compound prepar...

Embodiment 3

[0049] 1) Mix 10 parts of Astragalus root, 30 parts of Shanxiang round leaf, 10 parts of dandelion, 30 parts of Daqingye, 5 parts of Artemisia argyi, and five kinds of Chinese herbal medicines, add 3 times the volume of water and boil 3 times, 50min each time, combine and fry 3 times The liquid is boiled and concentrated to a compound Chinese herbal medicine concentrate with a crude drug content of 0.5g / mL.

[0050] 2) Add the compound Chinese herbal medicine prepared in step 1) into MRS broth to prepare a culture solution, adjust the pH value to 7.0, sterilize with high pressure steam at 115° C. for 30 minutes, and cool down to room temperature. Into the above-mentioned culture medium, and fermented at 37°C for 72 hours, the Chinese herbal medicine microecological compound preparation was prepared.

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Abstract

The invention provides a Chinese herbal medicine micro-ecological compound preparation for preventing and treating neonatal piglet enteritis and its preparation method and application, belonging to the technical field of veterinary traditional Chinese medicine and feed additive technology. It is prepared by adding the extract of Chinese herbal medicine to the liquid of Lactobacillus plantarum and Lactobacillus amylovora, and anaerobic fermentation; the weight ratio of the said lactobacillus liquid to the said Chinese herbal medicine is 1-3:40-50. Among them, the Chinese herbal medicines, in parts by weight, include: 2-8 parts of Astragalus membranaceus, 2-6 parts of Radix Sinensis, 2-6 parts of Dandelion, 2-6 parts of Daqingye, and 1-5 parts of Artemisia argyi. The Chinese herbal medicine micro-ecological compound preparation provided by the invention can be used in pig feed production, prevention and treatment of newborn piglet enteritis. Utilizing the synergistic effect between Lactobacillus and Chinese herbal medicine, it has the effects of improving the body's immunity, inhibiting harmful bacteria, and improving the intestinal microbial environment. It can effectively prevent piglet bacterial enteritis.

Description

technical field [0001] The invention relates to the technical field of veterinary traditional Chinese medicine and the technical field of feed additives, in particular to a Chinese herbal medicine microecological composite preparation for preventing and treating neonatal piglet enteritis, and a preparation method and application thereof. Background technique [0002] In recent years, with the advancement of large-scale pig farming, the existing problems have become more and more prominent. Better control of pig diseases, effectively improving the survival rate of piglets, and thus improving breeding income has become a need for research in pig breeding at this stage. one of the key issues. Diarrhea is one of the common diseases of piglets, which has a wide range of epidemics. It can reduce the daily gain and feed conversion rate of piglets, cause slow growth of piglets, and increase the mortality rate of piglets. It is the main factor that damages the growth performance of p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K10/12A23K10/30A23K50/30A61K36/481A61K36/704A61K36/855A61P1/00A61P1/12A61P37/04A61K35/744
CPCA23K10/12A23K10/30A23K50/30A61K36/481A61K36/185A61K36/288A61K36/855A61K36/704A61K36/315A61K36/195A61K36/282A61K35/744A61P1/12A61P1/00A61P37/04A61K2236/19A61K2300/00
Inventor 张锦华陶秀花吴允正王娇
Owner JIANGXI AGRICULTURAL UNIVERSITY
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