62 results about "Lactobacillus species" patented technology

The invention relates to fermented beverage of full cereal grains. The fermented beverage of full cereal grains is composed of the following ingredients by mass percent: 50-90% of fermentation broth of cereal grains, 8-14% of sucrose, 0.05-0.2% of citric acid, 0.05-0.1% of malic acid and the balance of pure water. The fermented beverage is prepared by mixing the ingredients, fine filtering the ingredients, homogenizing at the pressure of 18-25 Mpa, performing ultrahigh-temperature instant sterilization for 2-6 seconds at 135-137 DEG C, cooling to 80-85 DEG C and sterilely filling. The preparation process of the fermentation broth of cereal grains comprises the following steps of: adding alpha amylase in adjusted cereal grain pulp to perform enzymolysis at 50-65 DEG C, and inoculating specially domesticated lactic acid bacteria culture solution or powder to naturally ferment the zymolytic cereal grain pulp within 35-45 DEG C to obtain the fermentation broth of cereal grains. According to the invention, the development bottle neck of utilizing the cereal grains as food processing raw materials is solved through modern enzyme engineering and fermentation technology; and prebiotics are added in the beverage, and the dietary fiber content is within the range of 0.6-1.3mg / 100ml.

The invention relates to three major types of bacteria mixed liquid fermentation, solid yeast feed additive and a preparation method thereof and belongs to the field of microbial fermentation. The mixed bacteria yeast is prepared from lactic acid bacteria (five types of strain), saccharomycetes (three types of strain) and bacillus (six types of strain) in a mixing and fermenting mode. The preparation method for the mixed bacteria yeast feed additive includes that firstly, cultivating into mother strain through test tube slant culture medium and test tube fluid medium; secondly, performing shaking table oxygen consumption culture, slant solid culture and anaerobic triangular flask culture, and fermenting into liquid feed additive in a fermentation tank or inoculating to solid fermentation to form the feed additive after fermenting in the fermentation tank. The mixed bacteria yeast feed additive prepared by the method is natural, safe, environmental friendly, rich in metabolite, high in nutritive value and capable of providing the probiotic group needed for the intestines and stomachs of livestock and poultry, preventing diseases, improving the immunity of the livestock and poultry, reducing the harmful gas emission of the livestock and poultry and improving the environment of livestock and poultry houses. The preparation method is simple in culture medium prescription, capable of saving the production cost and lowering the fermentation energy consumption, high in fermentation yield and good in product quality.

A method of enhancing biomass yield of a lactic acid bacterial species cell culture, comprising cultivating the cells in a process comprising the steps of providing conditions that results in a reduced glycolytic flux and providing conditions that enable the cells to have, under aerobic conditions, a respiratory metabolism. The increased yield of biomass may be the result of an increased yield of ATP which can be obtained by activating the native ATP synthase activity of the H+-ATPase complex by lowering the ATP / ADP ratio, e.g. by carbon source limitation, and / or by increasing the proton gradient (membrane potential) of the cells, e.g. by enhancing or establishing an electron transport chain which can be achieved by enhancing expression of dehydrogenases or electron transport chain components, by adding to the medium a quinone or porphyrin compound or by enhancing the expression of the H+-ATPase activity.

The invention relates to a method for preparing fermented glutinous rice yoghurt. The method is characterized in that yoghurt and fermented glutinous rice are respectively fermented, produced, prepared under an aseptic condition, proportioned according to the proportion of the volume ratio of 1:1-2:1 and filled after being stirred, wherein, 0.1% of CMC-Na (sodium carboxymethylcellulose) is added into the milk base material of the yoghurt, lactobacillus species select Bulgaria lactobacilli and streptococcus thermophilus, and the activated Bulgaria lactobacilli and the activated streptococcus thermophilus are respectively inoculated in the prepared milk base material according to the addition quantity of the volume ratio of 2-5%. In the invention, the fermented glutinous rice yoghurt prepared after the two-time fermentation technique of glutinous rice fermentation and yoghurt fermentation has good stability, moderate sweetness and sourness, unique taste and slight granularity feel, is green and healthy, is a novel popular nutritional food integrating various advantages of the yoghurt and the fermented glutinous rice, has simple preparation technique, low price and abundant nutritionand can be preserved for 3 days at the low temperature of 4 DEG C.

The invention discloses a method for preparing probiotic fermented functional healthcare food with arctium lappa. The method includes extracting and separating main functional components such as arctium lappa inulin and dietary fibers in arctium lappa roots; screening probiotics capable of growing excellently by the aid of the arctium lappa inulin; screening excellent lactic acid bacterial culture in a high-throughput manner and developing high-density liquid fermentation technologies for the excellent lactic acid bacterial culture; mixing fermentation products, the extracted and separated dietary fibers and the like from the arctium lappa roots with one another and processing the fermentation products, the dietary fibers and the like to obtain the functional healthcare food with high additional value. The method has the advantages that the functional components in the arctium lappa roots are sufficiently utilized, pharmacological functions of the arctium lappa can be kept, and human body function regulating effects of the probiotics can be realized by the probiotic fermented functional healthcare food.

The present invention provides a pharmaceutical composition including a probiotic and a prebiotic for treatment of stunting in children. More particularly, the pharmaceutical composition includes at least one Lactobacillus specie(s) as a probiotic and at least one oligosaccharide as a prebiotic. The present invention further provides a method of treatment of stunting including administering an effective amount of a pharmaceutical composition including a probiotic and a prebiotic to children in need thereof.

The invention relates to a bran and red jujube juice lactic acid fermentation beverage prepared from the following raw materials in percentage by weight: 59%-65% of bran juice, 29%-34% of red jujube juice, 1.5%-2.5% of protein sugar, 1.2%-1.8% of sodium chloride, 0.2%-0.6% of glycerol and 2%-5% of lactobacillus strains liquid, wherein the bran juice contains 4.1% of soluble solid matters; and the red jujube juice contains 7% of the soluble solid matters. The bran and red jujube juice lactic acid fermentation beverage produced by enabling sweet and palatable red jujubes to be compatible with bran has very good palatability; meanwhile, the red jujube juice which is easy to culture microbes is added and the solubility of certain effective components contained in the bran is enhanced through lactic acid fermentation, thus the bran and red jujube juice lactic acid fermentation beverage is easier to digest and absorb; and in addition, the bran and red jujube juice lactic acid fermentation beverage is detected to have the reducing sugar content of 4.77 g / L, the lactic acid content of 1.51 g / L, the total nitrogen content of 0.31 g / L, a physicochemical index and a microbial index both achieving the GB19297-2003 'fruit and vegetable juice beverage' hygienic standard, thereby being used as a beverage.

The invention discloses a lactobacillus culture medium, a lactobacillus microbial fertilizer and application of the lactobacillus microbial fertilizer. The formula of the lactobacillus culture medium is as follows: a 3-4 g / L malt extract, a 7-10 g / L bone-marrow extract, 10-18 ml / L wood vinegar, a 15-30 g / L boiler dung raw material and 20-35 g / L molasses, has the pH of 4.8-5.2, and is prepared by water. The lactobacillus microbial fertilizer is prepared from the lactobacillus culture medium and a lactobacillus seed fluid. According to biological characteristics of lactobacillus, effective ingredients in broiler dung are reasonably utilized, so that agricultural lactobacillus can grow quickly to become a main flora, the production flow is simplified, the production cost is lowered, the microecological balance of soil can be promoted through application of the lactobacillus microbial fertilizer.

The invention relates to selenium-enriched health care thick wine containing cordyceps sinensis and mulberries. Fresh ripe selenium-enriched mulberries are picked, repeatedly washed, ground into a thick liquid and filtered, a soybean extract is added, the mixture is filtered and subjected to enzymolysis and juice filtration, and mulberry juice is obtained; glucose, a yeast extract and other nutrients are added to the mulberry juice, the mixture is sterilized, cordyceps sinensis is inoculated and cultured, and a cordyceps sinensis mycelium fermentation broth is obtained; after the fermentation broth is concentrated or not concentrated, white granulated sugar is added, the sugar degree is adjusted, a lactobacillus seed solution and high-activity dry yeast are added, after anaerobic fermentation is performed, filtration and clarification are performed, konjak oligosaccharides are added for blending, the mixture is filtered for sterilization, and the selenium-enriched health care thick wine containing the cordyceps sinensis and the mulberries is obtained. The formula is reasonable, and the thick wine has transparent color and luster, tastes sticky, refreshing and nutritious, has heavy ester fragrance and can reduce the blood glucose, relieve fatigue, improve the immunity of a human body, clear heat, remove internal heat, replenish blood, nourish yin, help produce saliva and slake thirst and adjust intestinal flora. Through measurement, the content of cordyceps sinensis polysaccharides is 0.76 g / 100 mL or higher, the content of cordycepin is 0.09 / 100 mL or higher, and the viscosity is higher than 200 mPa*s.

The invention relates to a probiotic freeze-dried shell composite protective agent and its application. The shell composite protective agent is composed of the following components in weight percent: 0.7-1.8% of isolated whey protein powder, 0.5-1.5% of tyrosine %, arginine 0.7-1.3%, gellan gum 0.1-1.1%, inulin 0.2-1.0%, vitamin C 0.2-0.8%, sodium alginate 0.1-0.5%, γ-polyglutamic acid 0.1-0.5% , The balance is distilled water. No chemical synthetic substances are added to the shell composite protective agent of the present invention, and the safety is high; the shell composite protective agent has a very significant protective effect on probiotics before and after the freeze-drying process; Both Lactobacillus and Lactobacillus strains have a good protective effect and have broad application prospects.

The invention discloses a method for preparing a rye fermentation product, which comprises the following steps: soaking the rye in water for germination, drying, pulverizing, and sieving to obtain rye flour; adding enzymes to the rye flour for enzymatic hydrolysis to obtain rye flour enzyme hydrolyzing liquid; adding nitrogen source and inorganic salt to the rye flour enzymatic hydrolyzing liquid to obtain a fermentation medium; inoculating the lactic acid bacteria seed liquid into the fermentation medium for fermentation, and after the fermentation is completed, a fermentation liquid is obtained; Liquid in addition to impurities, sterilization. The preparation method of the rye fermentation product provided by the invention has the advantages of short cycle, controllable process, simple and easy to enlarge process, stable product, good efficacy, easy absorption, safety and health, and conforms to the characteristics of cosmetic raw materials.