17 results about "Lactobacillus species" patented technology

The invention belongs to the technical field of preparation of probiotic products, and relates to a lactic acid bacteria microcapsule as well as a preparation method and application thereof. The method comprises the following steps: uniformly mixing sodium alginate, modified montmorillonite and sterile water, cooling, adding a prepared lactic acid bacteria seed solution, and fully and uniformly mixing to form an emulsion; spraying the emulsion into a calcium chloride solution by adopting an extrusion method, stirring and curing to obtain microcapsules; and culturing the prepared microcapsule in a lactic acid bacteria liquid culture medium to obtain the lactic acid bacteria microcapsule after the culture is finished. The lactobacillus is effectively protected by adopting an extrusion-method single coating and post-coating culture process, so that the stability of the lactobacillus to a humid and hot environment and the gastrointestinal stress resistance are improved, and meanwhile, the release performance of the lactobacillus in the gastrointestinal tract is ensured. The problem that an existing microcapsule coating technology cannot achieve balance between improvement of the hygrothermal stability and gastrointestinal stress resistance of the probiotics and guarantee of good release performance of the probiotics in the gastrointestinal tract is solved.

This invention relates to the field of lactic acid bacteria cultivation technology, and more particularly to a lactic acid bacteria seed culture and its preparation method. This invention utilizes citrus processing byproducts to construct a natural culture medium, eliminating the need for additional chemical reagents. This solves the problem of citrus processing byproduct disposal, achieving resource utilization of waste, and reduces the production cost of lactic acid bacteria cultivation. It also avoids the environmental deficiencies and poor cell protection effects of traditional artificially synthesized culture media, balancing the growth requirements of lactic acid bacteria with the feasibility of industrial applications. This model aligns with the concept of green development and promotes a closed-loop system in the citrus processing industry, achieving resource recycling. It not only meets the needs of large-scale lactic acid bacteria cultivation but also contributes to agricultural upgrading, alleviates resource waste and environmental pressure, and offers economic, ecological, and social benefits. It provides a new path for low-cost, green cultivation of lactic acid bacteria and injects new impetus into the sustainable development of the citrus processing industry.

The present invention relates to a method for culturing lactic acid bacteria that adapt to the vaginal environment and strongly adhere to vaginal epithelial cells. The method comprises inoculating the cultured lactic acid bacteria seed solution into a liquid culture medium, and culturing the culture medium statically until the OD value of the culture medium reaches 0. 600 When the value is 0.3-0.6, the preparation is added and culture is continued, and after completion, the lactic acid bacteria that strongly adhere to vaginal epithelial cells are obtained; the preparation includes L-arabinose. The present invention also relates to the use of L-arabinose in preparing a preparation for improving the adhesion of lactic acid bacteria to vaginal epithelial cells. By adding the relevant preparation during a specific growth stage of lactic acid bacteria, it is beneficial to ensure rapid bacterial proliferation and stable formation of surface hydrophobic proteins, thereby further improving the adhesion and colonization ability of the cultured lactic acid bacteria.

This invention relates to the field of functional food technology, and discloses a product combination and preparation method for promoting fat metabolism. The product combination is made from prickly pear juice, medicinal and edible raw materials, psyllium husk powder, honey, and yeast and lactic acid bacteria. It possesses a micron-level O / W-type Pickering emulsion structure, utilizing ultrafine pulverization to break down plant cell walls, exposing a rigid microparticle framework composed of lignin and cellulose, and endogenous free saponins as solid stabilizers, physically encapsulating hydrophobic essential oils. During preparation, the compound extract is hydrated and swelled with psyllium husk powder, then subjected to high-shear physical emulsification, followed by aerobic isothermal culture with activated yeast, and finally inoculated with high-enzyme-activity lactic acid bacteria seed liquid for isothermal anaerobic fermentation. This invention constructs a stable barrier to block essential oil oxidation and degrades polysaccharides to produce highly bioavailable small-molecule active ingredients and short-chain fatty acids, thereby improving the biotransformation efficiency for promoting fat metabolism.

The present invention pertains to a method for producing an antimicrobial composition preferably comprising piceol. Moreover, the invention relates to an antimicrobial composition comprising or consisting of piceol, preferably in an excess amount compared to picein, further plant-derived substances and optionally picein. The invention further relates to a cosmetic, personal care, household, home care, pet care or pharmaceutical product comprising said antimicrobial composition. Moreover, the invention relates to the use of a microorganism, preferably a lactobacillus species, for producing an antimicrobial composition comprising piceol from a composition, preferably a plant-extract, comprising picein. In addition, the invention relates to the use of shikimic acid for increasing the antimicrobial efficacy of piceol and / or to the use of piceol for increasing the antimicrobial efficacy of shikimic acid. Further embodiments and aspects of the invention are presented in the attached claims as well as in the description and examples below.

The present invention pertains to a method for producing an antimicrobial composition preferably comprising piceol. Moreover, the invention relates to an antimicrobial composition comprising or consisting of piceol, preferably in an excess amount compared to picein, further plant-derived substances and optionally picein. The invention further relates to a cosmetic, personal care, household, home care, pet care or pharmaceutical product comprising said antimicrobial composition. Moreover, the invention relates to the use of a microorganism, preferably a lactobacillus species, for producing an antimicrobial composition comprising piceol from a composition, preferably a plant-extract, comprising picein. In addition, the invention relates to the use of shikimic acid for increasing the antimicrobial efficacy of piceol and / or to the use of piceol for increasing the antimicrobial efficacy of shikimic acid. Further embodiments and aspects of the invention are presented in the attached claims as well as in the description and examples below.

Methods and compositions for modulating a microbiome of a urogenital area or a skin area of a user are disclosed. The method can include applying a composition to a urogenital area or a skin area of a user, wherein the composition can include a carrier and a therapeutic agent. The therapeutic agent can include at least one prebiotic comprising at least one high molecular weight dextrin with two or more α-1,6-glycosidic bonds, with or without a cyclic moiety. The at least one high molecular weight dextrin with two or more α-1,6-glycosidic bonds comprises a highly branched cyclic dextrin, a β-limit dextrin, or a combination thereof. The method can further include promoting a healthy microflora balance of the urogenital area or the skin area of the user. Further disclosed is a composition for modulating a microbiome of a urogenital area or a skin area of a user, wherein the composition comprises a single liquid phase, the single liquid phase comprising an aqueous solution and a therapeutic agent. The therapeutic agent can include at least one prebiotic compound comprising at least one high molecular weight dextrin with two or more α-1,6-glycosidic bonds, with or without a cyclic moiety, such as a highly branched cyclic dextrin, a β-limit dextrin, or a combination thereof. The disclosed methods and compositions for modulating a microbiome of a urogenital area or a skin area of a user help promote the growth of healthy Lactobacillus species to prevent dysbiosis and other related conditions and infections.

The invention discloses an application of a bacterial strain in production of an external product for improving the micro-ecology of the vagina of a human body, and the bacterial strain is Lactobacillus crispatus SPR002 and has 16S rDNA (ribosomal deoxyribonucleic acid) as shown in SEQ ID NO.1. The invention also discloses an application of the bacterial strain in production of the external product for improving the micro-ecology of the vagina of the human body. The strain produces H2O2 and only produces D-lactic acid, and no drug-resistant gene is found; the external product is suitable for improving changes, caused by vaginal pathogenic bacteria, of micro-ecology of the vagina of the human body. When the strain concentration is 1 * 10 < 7 > CFU / mL, the strain SPR002 provided by the invention can generate a strong bacteriostatic effect on staphylococcus aureus, and the bacteriostatic rate reaches 98.15%; the bacteriostasis rate of the bacterial strain SPR002 to candida albicans can be obviously improved along with the increase of the concentration of the bacterial strain SPR002, and when the concentration of the bacterial strain is 3 * 10 < 8 > CFU / mL, the bacteriostasis rate of the bacterial strain SPR002 to candida albicans reaches 97.1%. Compared with other Lactobacillus crispatus of the same type, the strain SPR002 provided by the invention can generate a relatively strong inhibition effect on Candida albicans along with the increase of the concentration, so that the strain SPR002 provided by the invention can be developed into a medicine product, and the dosage of the medicine is appropriately increased according to the condition of a patient.

The present invention provides compositions and methods for enhancing the barrier function of surfaces and / or objects using biochemical-producing microorganisms and / or by-products synthesized by such microorganisms. [Solution] A composition is provided comprising a Lactobacillus species grown as a biofilm and / or an extract of the biofilm, wherein when the composition is applied to a surface, the biofilm and / or extract enhance the barrier function such that the surface is less susceptible to the effects of microorganisms, viruses, and / or chemical contamination.

The invention provides an animal microecological preparation with an anti-stress function and a preparation method thereof, and belongs to the technical field of microecological preparations, and the preparation method comprises the following steps: culturing bacillus subtilis and lactic acid bacteria to obtain a bacillus seed solution and a lactic acid bacteria seed solution, and carrying out aerobic fermentation on the bacillus seed solution to obtain a fermentation liquid; adding a lactic acid bacteria seed solution for micro-aerobic fermentation; culturing saccharomyces cerevisiae to obtain a saccharomyces cerevisiae seed solution, mixing the fermentation product with the saccharomyces cerevisiae seed solution, and sequentially centrifuging and freeze-drying to obtain freeze-dried bacterial powder; the preparation method comprises the following steps: mixing freeze-dried bacterial powder, prebiotics, chlorogenic acid, L-glutamine, yeast beta-glucan and deionized water to obtain a core material solution, mixing the core material solution with a sodium alginate solution, dropwise adding into a calcium chloride solution for crosslinking, soaking in a chitosan solution, soaking in a sodium tripolyphosphate solution again for crosslinking, and carrying out spray drying. Through synergistic fermentation of multiple strains and composite embedding of functional components, the anti-stress function of the animal microecological preparation is improved.

The invention relates to the technical field of sour slurry noodles, and particularly discloses a preparation process of pre-packaged instant sour slurry noodles, and the process comprises the steps of sour slurry preparation, noodle preparation, stir-fried minced meat preparation, sterile filling, pre-packaging combination and the like. And precise temperature control and semi-closed fermentation management are combined, so that directional fermentation which is dominated by lactic acid bacteria is successfully guided. According to the sterile filling minced meat sauce bag, the raw materials are added, bad water caused by infectious microbe pollution is avoided, astringent sour pulp seasoning juice is stably produced, the pH value of the sour pulp seasoning juice can be stably controlled within the optimal eating range of 3.5-5.0, the complex flavor of traditional natural fermentation is restored to the maximum extent, and meanwhile the sterile filling minced meat sauce bag has the advantages of being free of Chinese sauerkraut addition, sterile filling in the whole process and sealing detection technology. The problem that the traditional pickled Chinese cabbage-containing minced meat is rotten next day is solved; raw material loss and storage cost are reduced, and support is provided for large-scale storage and transportation.

Methods and compositions for modulating the microbiome of the urogenital region or skin region of a user are disclosed. The method may include applying a composition to a urogenital region or skin region of a user, wherein the composition may include a carrier and a therapeutic agent. The therapeutic agent may comprise at least one prebiotic comprising at least one high molecular weight dextrin having two or more alpha-1, 6-glycosidic bonds, with or without a cyclic moiety. The at least one high molecular weight dextrin having two or more alpha-1, 6-glycosidic bonds comprises a highly branched cyclodextrine, a beta-limit dextrin, or a combination thereof. The method may also include promoting healthy microbiota balance in the urogenital region or skin region of the user. Further disclosed is a composition for modulating the microbiome of the urogenital region or skin region of a user wherein the composition comprises a single liquid phase comprising an aqueous solution and a therapeutic agent. The therapeutic agent may comprise at least one prebiotic compound comprising at least one high molecular weight dextrin having two or more alpha-1, 6-glycosidic bonds, having or not having a cyclic moiety, such as a highly branched cyclodextrin, a beta-limit dextrin, or a combination thereof. The disclosed methods and compositions for modulating the microbiome in the urogenital region or skin region of a user contribute to promoting the growth of healthy Lactobacillus species, thereby preventing dysbiosis and other related conditions and infections.

The application provides an animal microecological preparation with stress resistance and a preparation method thereof, and belongs to the technical field of microecological preparations, and comprises the following steps: culturing Bacillus subtilis and lactic acid bacteria to obtain Bacillus subtilis seed liquid and lactic acid bacteria seed liquid, carrying out aerobic fermentation on the Bacillus subtilis seed liquid, and adding the lactic acid bacteria seed liquid to carry out micro-aerobic fermentation; culturing Saccharomyces cerevisiae to obtain yeast seed liquid; after the fermentation product and the yeast seed liquid are mixed, centrifugation and freeze-drying are sequentially carried out to obtain freeze-dried bacterial powder; the freeze-dried bacterial powder, prebiotics, chlorogenic acid, L-glutamine, yeast beta-glucan and deionized water are mixed to obtain a core solution; after the core solution and a sodium alginate solution are mixed, the mixture is dropped into a calcium chloride solution to carry out cross-linking, the mixture is soaked in a chitosan solution, and then the mixture is soaked in a trisodium phosphate solution to carry out cross-linking, and finally, the mixture is subjected to spray drying. Through the composite embedding of multi-strain synergistic fermentation and functional components, the animal microecological preparation is improved in the stress resistance.

The present invention relates to the efficient delivery of anti-infective activity, immune modulators, or growth-promoting biomolecules directly to the gastrointestinal tract of animals via a biodelivery platform.SOLUTION: The biodelivery platform can be a genetically modified microorganism. Delivery can be achieved using Lactobacillus species fix in the gastrointestinal tract. The anti-infective activity can be a bactericidal or bacteriostatic peptide, an antibody or fragment thereof that specifically recognizes a pathogen, or a phage or lytic peptide derived from a phage that specifically targets a particular pathogen.SELECTED DRAWING: None

This invention relates to compositions comprising an effective amount of bacterial strains of the genera Lacticaseibacillus and Lactobacillus as well as methods and uses of said compositions for promoting growth and metabolism of vaginal Lactobacillus species in a subject in need thereof.

Compositions and methods for treating patients are disclosed. An example composition may include a vaginal microbiota composition. The vaginal microbiota composition may include a mixture of bacteria. The mixture of bacteria may include Lactobacillus crispatus, Lactobacillus gasseri, Lactobacillus jensenii / Lactobacillus mulieris and Lactobacillus rhamnosus.