Fermentation technology for producing calcium lactate with sweet potatoes as raw material
A production process and technology of calcium lactate, applied in the directions of fermentation, microorganism-based methods, microorganisms, etc., can solve the problems of high cost, environmental pollution, time-consuming and labor-intensive, etc., and achieve the effects of low production cost, high product yield, and simple process.
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Embodiment 1
[0020] 1. Take out a kind of Lactobacillus acidophilus (Lactobacillus acidophilus, strain number: kaif6006) preserved at low temperature, thaw it at room temperature, and insert it into 100ml lactic acid strain culture medium. The strain culture medium composition is: 5 ° Bé Add 0.5% yeast extract, 0.6% sterile calcium carbonate, 1.5-2.0% agar to the wort, pH6.8. After maintaining the temperature in the incubator at 48-50°C for 6 hours, add an appropriate amount of CaCO 3 , keep the pH value of the culture medium at 5.5-6.0, ferment for 24-28 hours, and then gradually expand the culture to 500mL, 3000mL, 25L until 2000L strain tank according to the above steps.
[0021] 2. At 50m 3 In the fermentation tank, add 3 / 4 of the canned amount of dried potato hydrolyzed sugar solution made by liquefaction and saccharification, the concentration of the sugar solution is 15%, keep the temperature at 48-50°C, add 5% wheat bran, and make the fermentation liquid .
[0022] 3. Expand the...
Embodiment 2
[0026] 1. Take out a kind of Lactobacillus delbrueckii subsp (Lactobacillus delbrueckii subsp, strain number: kaif6004) preserved at low temperature, thaw it at room temperature, and insert it into 100ml lactic acid strain culture medium. The strain culture medium is composed of casein peptone 10.0g, beef extract 10.0g, yeast powder 5.0g, glucose 5.0g, sodium acetate 5.0g, diamine citrate 2.0g, Tween801.0g, K 2 HPO 4 2.0g, MgSO 4 ·7H 2 O0.2g, MnSO 4 ·H 2 O 0.05g, CaCO 3 20.0g, 15.0g agar, 1.0L distilled water, pH 6.8. After maintaining the temperature in the incubator at 48-50°C for 6 hours, add an appropriate amount of CaCO 3 , keep the pH value of the culture medium at 5.5-6.0, ferment for 24-28 hours, and then gradually expand the culture to 500mL, 3000mL, 25L until 2000L strain tank according to the above steps.
[0027] 2. At 50m 3 In the fermenter, add 3 / 4 of the canned amount of dried potato hydrolyzed sugar solution produced by liquefaction and saccharificatio...
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