Preparation method for producing acidification glycolysis feed by asynchronously fermenting mixed strains

A technology of asynchronous fermentation and bacterial fermentation, which is applied in the fields of feed, enzyme preparation and microorganism

Inactive Publication Date: 2016-12-07
FLSUGARPEPTIDE BIOLOGY ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, none of the preparation methods of the above pending invention patents integrate lactic acid bacteria and Aspergillus niger together for fermentation, that is, they do not first inoculate lactic acid bacteria to carry out anaerobic enzymatic fermentati

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  • Preparation method for producing acidification glycolysis feed by asynchronously fermenting mixed strains

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[0122] Example 1

[0123] A preparation method of mixed bacteria asynchronous fermentation to produce acidified and fermented feed includes the following steps:

[0124] ① Preparation of liquid culture medium:

[0125] 100kg of corn was crushed, 200kg of water was added to adjust the slurry, and amylase was added for enzymatic hydrolysis, liquefaction, and saccharification to obtain 300kg of liquid culture medium with residue.

[0126] ② Preparation of wet base of liquid lactic acid bacteria fermentation medium:

[0127] In 300kg of liquid medium with residue mixed with 0.08kg of complex enzyme (the weight ratio of xylanase: glucanase: mannanase: pectinase: protease is 100:60:60:60:30), Connect 30kg of lactic acid bacteria seed liquid, carry out anaerobic fermentation under the conditions of fermentation temperature 42-45°C and initial fermentation pH of 6-7 for 48 hours to prepare 328kg liquid lactic acid bacteria fermentation medium wet base.

[0128] ③ Preparation of wet base of liqu...

Example Embodiment

[0132] Example 2

[0133] A preparation method of mixed bacteria asynchronous fermentation to produce acidified and fermented feed includes the following steps:

[0134] ① Preparation of solid culture medium:

[0135] 100kg of corn was crushed, 50kg of water was added and stirred, and amylase was added for enzymatic hydrolysis, liquefaction and saccharification to obtain 150kg of saccharified solid medium.

[0136] ② Preparation of wet base of solid lactic acid bacteria fermentation medium:

[0137] Mix 0.10kg of complex enzyme (xylanase: glucanase: mannanase: pectinase with a weight ratio of 100:80:60:60) in 150kg saccharified solid medium, and add 50kg of lactic acid bacteria Seed liquid, under the conditions of fermentation temperature 42-45°C and initial fermentation pH of 6-7, carry out anaerobic fermentation for 72 hours to prepare 185kg solid lactic acid bacteria fermentation medium wet base.

[0138] ③ Preparation of wet base of solid citric acid bacteria fermentation medium:

[...

Example Embodiment

[0143] Example 3

[0144] A preparation method of mixed bacteria asynchronous fermentation to produce acidified and fermented feed includes the following steps:

[0145] ① Preparation of liquid culture medium:

[0146] 100kg of corn was crushed, 200kg of water was added to adjust the slurry, and amylase was added for enzymatic hydrolysis, liquefaction, and saccharification to obtain 300kg of liquid culture medium with residue.

[0147] ② Preparation of wet base of liquid lactic acid bacteria fermentation medium:

[0148] In 300kg of liquid medium with residue mixed with 0.08kg of complex enzyme (the weight ratio of xylanase: glucanase: mannanase: pectinase: protease is 100:60:60:60:30), Connect 30kg of lactic acid bacteria seed liquid, carry out anaerobic fermentation under the conditions of fermentation temperature 42-45°C and initial fermentation pH of 6-7 for 48 hours to prepare 328kg liquid lactic acid bacteria fermentation medium wet base.

[0149] ③ Preparation of wet base of liqu...

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Abstract

The invention relates to a preparation method for producing acidification glycolysis feed by asynchronously fermenting mixed strains. The preparation method includes the steps: performing beating, mixing and enzymolysis for sugar containing products to obtain a liquid culture medium with residues; firstly inoculating lactic strains into the liquid culture medium, performing fermentation for several hours, then inoculating citric acid strains, performing fermentation for several hours, and asynchronously fermenting the mixed strains to produce a liquid fermenting culture medium wet base; separating solid and liquid in the liquid fermenting culture medium wet base to obtain an acid residue wet base and composite acid dilute liquid; performing evaporation and concentration for the composite acid dilute liquid to obtain composite acid concentrated liquid, mixing one or more of clean water, a sugar residue wet base, the acid residue wet base, feed stuffs, composite strain liquid and composite enzymes, and then performing solid fermentation to obtain a glycolysis residue feed wet base; mixing the composite acid concentrated liquid and the glycolysis residue feed wet base to obtain an acidification glycolysis feed wet base, or drying the composite acid concentrated liquid and the glycolysis residue feed wet base to make an acidification glycolysis feed dry base. The prepared feed has nutrients such as citric acid, lactic acid and oligosaccharide and has the advantages of diarrhea resistance, good palatability, easiness in digestion and the like.

Description

technical field [0001] The invention belongs to the technical fields of feed, enzyme preparations and microorganisms, and in particular relates to a preparation method for asynchronous fermentation of mixed bacteria to produce acidified fermented feed. Background technique [0002] In recent years, food safety and environmental pollution have been widely concerned by governments at all levels in various countries, which are related to the health and life safety of the people. The safety of animal products requires that the feed has no drug residues, and the environmental protection requires that the feed has low pollution. In the past, we talked about "effective and safe" feed, but now the Ministry of Agriculture has repeatedly emphasized "safe, effective and low pollution". As we all know, since the advent of antibiotics, their use in livestock and poultry to prevent diseases and stimulate growth has indeed received good results. However, with the passage of time, the defe...

Claims

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Application Information

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IPC IPC(8): A23K50/30A23K10/10A23K40/00
Inventor 程勐万里刘萱仪孟龙薛芹程茂基
Owner FLSUGARPEPTIDE BIOLOGY ENG
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