Method for detecting variety of lactic acid bacteria strain through common or fluorescent quantitation PCR

Abstract

The present invention discloses a method for detecting the variety of lactic acid bacteria strain through common or fluorescent quantitation PCR. The method comprises: downloading all known bacteria associated database information from NCBI; finding out data of four varieties of lactic acid bacteria, and carrying out sequence comparison to find out the specific sequence of the variety; designing primers of the found sequence, and carrying out primary specific primer screening; and designing fluorescent quantitation PCR primers by using the specific fragment detected by PCR so as to determine the variety of the species to be detected. According to the present invention, the important purpose of the present invention is to distinguish the lactic acid bacteria subspecies, identify the variety and the purity of the lactic acid bacteria, and easily and quickly detect the variety of the four lactic acid bacteria species such as Lactobacillus casei, Lactobacillus delbrueckii, Lactobacillus fermentum and Lactobacillus plantarum.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a method for detecting lactic acid bacteria species by ordinary or fluorescent quantitative PCR. Background technique [0002] Lactic acid bacteria refers to the general term for a class of non-spore-forming bacteria that produce lactic acid by fermenting sugars. They are widely distributed in nature and are ideal materials for studying classification, biochemistry, genetics, molecular biology and genetic engineering. They have important academic value in theory. , and the application value of lactic acid bacteria in important fields closely related to human life such as industry, agriculture and animal husbandry, food and medicine is also extremely high. Lactic acid bacteria can prevent lactose intolerance, promote the absorption of calcium, magnesium, protein, monosaccharide and other nutrients, produce beneficial substances, increase intestinal benefic...

AI Technical Summary

Problems solved by technology

Due to the wide variety of lactic acid bacteria, it is difficult to detect and identify them with common methods in the prior art. At the same time, they are widely used in production and life. Therefore, it is particularly important to carry out qualitative and quantitative detection of lactic acid bacteria by biological means.