Method for preparing feed raw material by fermenting rice vinasse with lactic acid bacteria

A technology for lactic acid bacteria fermentation and feed raw materials, which is applied in the field of preparing feed raw materials by using lactic acid bacteria to ferment rice distiller's grains, can solve the problems of lack of comprehensive utilization of rice distiller's grains, etc., and achieve the effects of improving intestinal microenvironment, increasing feed intake, and improving palatability.

Pending Publication Date: 2020-10-30
卢卫红
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The first purpose of the present invention is to solve the problem of lack of comprehensive utilization of rice distiller's grains in the prior art, and provide a method for preparing feed raw materials by using lactic acid bacteria to ferment rice distiller's grains

Method used

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  • Method for preparing feed raw material by fermenting rice vinasse with lactic acid bacteria

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Embodiment 1

[0021] A method for preparing feed raw materials by using lactic acid bacteria to ferment rice distiller's grains, comprising the following steps:

[0022] Step 1: Preparation of lactic acid bacteria seed liquid: Inoculate 1 mL of Lactobacillus acidophilus glycerol strain into 150 mL MRS liquid medium, 1 mL of Lactobacillus caseioid glycerol strain in 150 mL MRS liquid medium, and Enterococcus faecalis glycerol strain 1 mL Inoculate in 150mL MRS liquid medium, inoculate 1mL of Lactobacillus plantarum glycerol tube strain into 150mL MRS liquid medium, culture at 37°C and 150rpm for 24 hours, and obtain four kinds of lactic acid bacteria seed liquids, namely Lactobacillus acidophilus seed liquid, cheese-like Lactobacillus seed liquid, Enterococcus faecalis seed liquid and Lactobacillus plantarum seed liquid;

[0023] Step 2: Pretreatment of rice distiller's grains: (1) use MVR evaporator to concentrate rice distiller's grains to a total solid content of 14%, to obtain feed liqui...

Embodiment 2

[0027] A method for preparing feed raw materials by using lactic acid bacteria to ferment rice distiller's grains, comprising the following steps:

[0028] Step 1. Preparation of lactic acid bacteria seed solution: inoculate 1 mL of Enterococcus faecium glycerol strain into 150 mL MRS liquid medium, inoculate 1 mL of Lactobacillus casei glycerol strain into 150 mL MRS liquid medium, and inoculate 1 mL of Enterococcus faecalis glycerol strain In 150mL MRS liquid medium, 1mL of Lactobacillus plantarum glycerol tube strain was inoculated in 150mL MRS liquid medium, and cultured with shaking at 37°C and 150rpm for 24 hours to obtain four kinds of lactic acid bacteria seed liquids, namely Enterococcus faecium seed liquid and Lactobacillus paracasei Seed liquid, Enterococcus faecalis seed liquid and Lactobacillus plantarum seed liquid;

[0029] Step 2, pretreatment of rice distiller's grains: (1) use MVR evaporator to concentrate rice distiller's grains to a total solid content of 1...

Embodiment 3

[0033] A method for preparing feed raw materials by using lactic acid bacteria to ferment rice distiller's grains, comprising the following steps:

[0034] Step 1. Preparation of lactic acid bacteria seed liquid: Inoculate 1 mL of Lactobacillus plantarum glycerol strain into 150 mL MRS liquid medium, 1 mL of Lactobacillus caseioid glycerol strain into 150 mL MRS liquid medium, and inoculate 1 mL of Enterococcus faecalis glycerol strain In 150mL of MRS liquid medium, cultured at 37°C and 150rpm for 24 hours with shaking to obtain three kinds of lactic acid bacteria seed liquids, namely Lactobacillus plantarum seed liquid, Lactobacillus paracasei seed liquid and Enterococcus faecalis seed liquid;

[0035] Step 2, pretreatment of rice distiller's grains: (1) use MVR evaporator to concentrate rice distiller's grains to a total solid content of 16%, and obtain feed liquid I; (2) adjust the pH of feed liquid I to 5.8 with ammonia solution to obtain feed Liquid II; (3) add 70% wheat ...

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Abstract

The invention relates to a method for preparing a feed raw material by fermenting rice vinasse with lactic acid bacteria, which belongs to the field of biological fermentation feeds, and specificallycomprises the following steps: step 1, inoculating lactic acid bacteria into an MRS liquid culture medium, and carrying out activation culture to obtain a lactic acid bacteria seed solution; step 2, concentrating the rice wine lees to obtain a feed liquid I, adjusting the pH value of the feed liquid I to obtain a feed liquid II, adding auxiliary materials into the feed liquid II, and uniformly mixing to obtain a material III; and 3, inoculating the lactic acid bacteria seed solution prepared in the step 1 into the material III obtained in the step 2, and fermenting to obtain the feed raw material. According to the invention, high-valued comprehensive utilization is carried out on the rice wine lees; the probiotics fermented feed containing various active lactic acid bacteria, small molecule polypeptide and antibiotics is developed by using the rice vinasse as a nutrient solution and utilizing a microbial fermentation technology, the problem that the rice vinasse is difficult to biochemically treat is solved, the additional value of the rice vinasse is increased, and the probiotics fermented feed has remarkable economic benefits and social benefits.

Description

technical field [0001] The invention belongs to the field of biologically fermented feed, and in particular relates to a method for preparing feed raw materials by using lactic acid bacteria to ferment rice distiller's grains. Background technique [0002] Distiller's grains are a by-product of the brewing industry, and the amount is large and concentrated. According to statistics, my country's annual output of various distiller's grains is tens of millions of tons, divided into white distiller's grains, beer's grains, and rice distiller's grains, among which the annual output of rice distiller's grains is about 500,000-1 million Tons, rice distiller's grains are wastes left after brewing → distillation of rice wine in Guangdong and Guangxi, with a water content of about 90%, which is the highest among all distiller's grains, difficult to store, high acidity (pH3.4-3.8), very easy to deteriorate and rancid; As high-concentration organic wastewater, its COD>30000mg / L, if it ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/12A23K10/38
CPCA23K10/12A23K10/38A23V2400/165A23V2400/113A23V2400/169Y02P60/87
Inventor 卢卫红
Owner 卢卫红
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