Preparation method and application of lactic acid bacteria capable of inhibiting tyrosinase activity

Abstract

The invention provides a preparation method and application of lactic acid bacteria capable of inhibiting tyrosinase activity. The preparation method comprises the following steps: obtaining an original strain from yoghourt or infant intestinal tracts by separation and purification; culturing the original strain through an improved MRS liquid culture medium to obtain an improved strain; uniformlymixing the improved strain with 30% by mass of glycerol, and performing cryopreservation at -80 DEG C to obtain a lactic acid bacteria seed solution; and fermenting the lactic acid bacteria seed solution: concretely inoculating 10 mL of the improved MRS liquid culture medium with the lactic acid bacteria seed solution according to an inoculation amount of 3%, performing uniform mixing by shaking,performing culturing at 37 DEG C for 24 hours, sequentially and continuously performing activating for three generations, and inoculating 250 ml of an improved MRS liquid culture medium with the lactic acid bacteria seed solution according to an inoculation amount of 3% for amplification culture to obtain a fermentation liquid. Six related strains and derivatives by the invention all have effectsof inhibiting tyrosinase activity, and the fermentation liquid and the supernatant can be used for whitening products after inactivation and desalting treatment. Dead bacteria LR863 and LR519 have good effect of inhibiting tyrosinase activity, and can be directly used for whitening skin care products, wherein the recommended use concentration is 1-5 billion strains/ml.

Description

technical field [0001] The invention relates to the field of microorganisms; in particular, it relates to a preparation method and application of lactic acid bacteria with activity of inhibiting tyrosinase. Background technique [0002] The color of human skin is determined by the amount of melanin produced by the human body. At the same time, melanin is also an essential biotin for human skin to protect against ultraviolet radiation. Its excessive abnormal metabolism in the basal layer of the skin leads to the accumulation of melanin or skin pigmentation, which makes the skin become darker. It was dull and black. [0003] There are four internal factors affecting melanin production: tyrosinase, dopachrome interconverter, 5,6-dihydroxyindole-2-carboxylate oxidase, and melanocyte-stimulating hormone. Under aerobic conditions, tyrosinase oxidizes L-tyrosine to L-dopa, and then oxidizes to the corresponding quinone. Under the action of dopachrome interconversion enzyme, dopaqu...

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Problems solved by technology

However, because of its corrosiveness, cytotoxicity and allergies to the skin, it has certain dangers and has been banned in many countries' health regulations.

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