Lactobacillus culture medium, lactobacillus microbial fertilizer and application of lactobacillus microbial fertilizer

A technology of microbial fertilizer and lactic acid bacteria, applied in the directions of microorganism-based methods, microorganisms, applications, etc., can solve the problems of complex production process, slow growth rate of agricultural lactic acid bacteria, high production cost, simplify production process, promote soil micro-ecological balance, The effect of reducing production costs

Active Publication Date: 2017-11-03
抚顺茂百特生态环保科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of this, the object of the present invention is to provide a kind of lactic acid bacteria culture medium, lactic acid bacteria microbial fertilizer and application thereof, to solve the slow growth rate of agricultural lactic acid bacteria, complex production process, high production cost and other problems

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Select 1 kg of fresh broiler manure with a water content of 84.7%, add 3 kg of water and stir for 15 minutes to remove suspended feathers and sundries, remove sand particles at the bottom, take the upper suspension, squeeze and dehydrate through a 50-mesh filter, and then disperse Open it into the drying oven, dry at 100°C for 30 minutes, and obtain 0.37kg of broiler manure treatment raw material with a water content of 30%;

[0023] 2. Take 1000mL of water and heat to dissolve 21g of malt extract, 70g of bone marrow extract, and 245g of molasses, then add 105g of broiler manure treatment raw material obtained in step 1, stir well and add to the fermentation container, add water to 7000ml, and then add Wood vinegar 126ml, obtains lactic acid bacteria culture medium;

[0024] 3. Take 2100mL of cultured lactic acid bacteria seed solution and add it to the lactic acid bacteria medium prepared in step 2, and culture it anaerobically at 27°C for 30 days until no bubbles a...

Embodiment 2

[0026] 1. Select 1 kg of fresh broiler manure with a water content of 84.7%, add 4 kg of water and stir for 20 minutes, remove suspended feathers and sundries, remove sand particles at the bottom, take the upper suspension, squeeze and dehydrate through a 50-mesh filter, and then disperse Open it into the drying oven, dry at 110°C for 25 minutes, and obtain 0.40 kg of broiler manure treatment raw material with a water content of 40%;

[0027] 2. Take 1000mL of water and heat to dissolve 28g of malt extract, 49g of bone marrow extract, and 140g of molasses, then add 210g of broiler manure treatment raw material obtained in step 1, stir well and add to the fermentation container, add water to 7000ml, and then add 70ml of wood vinegar to obtain a lactic acid bacteria culture medium;

[0028] 3. Take 1750mL of cultured lactic acid bacteria seed solution and add it to the lactic acid bacteria culture medium prepared in step 2, and culture it anaerobically at 30°C for 35 days until ...

Embodiment 3

[0030] 1. Select 300kg of fresh broiler manure with a water content of 71.8%, add 1100kg of water and stir for 15 minutes, remove suspended feathers and sundries, remove sand at the bottom, take the upper suspension, squeeze and dehydrate it through a 50-mesh filter, and send it to Put into the drying drum, dry at 105°C for 30min, and obtain 95.3kg of broiler manure treatment raw materials with a water content of 39.7%;

[0031] 2. Take 200L of water and heat to dissolve 3.5kg of malt extract, 8kg of bone marrow extract, and 30kg of molasses, then add 25kg of broiler manure treatment raw materials obtained in step 1, stir well, add to the fermentation container, add water to 1000L, and then Add 15L of wood vinegar to obtain a lactic acid bacteria culture medium;

[0032] 3. Take 300L of the cultured lactic acid bacteria seed liquid and add it to the fermentation container equipped with the lactic acid bacteria culture medium prepared in step 2, and culture it anaerobically at ...

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PUM

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Abstract

The invention discloses a lactobacillus culture medium, a lactobacillus microbial fertilizer and application of the lactobacillus microbial fertilizer. The formula of the lactobacillus culture medium is as follows: a 3-4 g / L malt extract, a 7-10 g / L bone-marrow extract, 10-18 ml / L wood vinegar, a 15-30 g / L boiler dung raw material and 20-35 g / L molasses, has the pH of 4.8-5.2, and is prepared by water. The lactobacillus microbial fertilizer is prepared from the lactobacillus culture medium and a lactobacillus seed fluid. According to biological characteristics of lactobacillus, effective ingredients in broiler dung are reasonably utilized, so that agricultural lactobacillus can grow quickly to become a main flora, the production flow is simplified, the production cost is lowered, the microecological balance of soil can be promoted through application of the lactobacillus microbial fertilizer.

Description

technical field [0001] The invention relates to the technical field of microorganism cultivation and application, and in particular provides a lactic acid bacteria culture medium, a lactic acid bacteria microbial fertilizer and applications thereof. Background technique [0002] Microbial fertilizer contains a large number of beneficial microorganisms, and its main function is to realize the transformation between substances and the formation of beneficial metabolites through the life activities of beneficial microorganisms. Microbial fertilizers can activate soil, fix nitrogen, improve crop nutritional conditions, and promote crop growth by mediating a good soil micro-ecological environment. Today, with the continuous development of agricultural science and technology, modern agricultural technology has brought great contributions to my country's agricultural production, but at the same time, due to the extensive use of pesticides and chemical fertilizers, it has also cause...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C09K17/14C05F11/08C12R1/01
CPCC05F11/08C09K17/14C12N1/20Y02W30/40
Inventor 马莲菊陶思源卜宁李雪梅李玥莹王兰兰王泽王金缘张韫璐徐萌胡金丽周振宇
Owner 抚顺茂百特生态环保科技有限公司
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