Method for continuously preparing lactic acid bacterial liquid for fermented feed

A technology of lactic acid bacteria liquid and fermented feed, which is applied in the direction of animal feed, animal feed, bacteria, etc., can solve the problems of high production cost of fermented feed, large investment in equipment, high cost of cultivation, etc., and achieve short cultivation cycle, short production cycle and process simple effect

Active Publication Date: 2015-12-09
连云港九洲科技饲料有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the high production cost of fermented feed, according to incomplete statistics, there is currently only about 500,000 tons of fermented protein feed, which is still a big gap compared with the approximately 50 million tons of protein feed raw materials in the feed industry.
[0006] At present, the lactic acid bacteria liquid used for feed fermentation is prepared by step-by-step expansion cultivation of more than three levels, which has a long life cycle (about 72 hours) and a large investment in equipment (for example, feeding fermented feed every day, it needs to be equipped with 3 sets of seed expansion cultivation equipment) and High cost of culture (synthetic medium, multiple steam sterilization) and other deficiencies

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: prepare lactic acid bacteria bacterial liquid according to the following method

[0027] Use soybean meal (30%), bran (40%) and corn flour (30%) as the main raw materials, after stirring evenly, sterilize at 121°C for 30 minutes, add 5% (v / w) pentose sugar according to the weight of the solid matrix The bacterium liquid of Pediococcus CGMCCNo.9182 and the sterilized water of 95% (v / w), mix homogeneously, pack into aseptic aspect ratio and be in the fermentation container of 5: 1 (bottom sieve plate, high-purity ultra-high Molecular weight polyethylene), after cultivating at 35°C for 24h, wash with 0.5% sterile glucose solution of amylase containing 200U / g protein feedstuff with 20 volumes of acidic protease and 400U / g starchy feedstuff, and the effluent Collected into a culture tank with stirring, cultured at 37°C, 30r / min for 4h, the number of lactic acid bacteria reached 5.2×10 9 Individuals / mL or more. The solid-state fermentation substrate was washed...

Embodiment 2

[0028] Embodiment 2: prepare lactic acid bacteria bacterial liquid according to the following method

[0029] Use 30 parts of cottonseed meal, 40 parts of bran and 30 parts of corn flour as the main raw materials. After stirring evenly, sterilize at 121°C for 30 minutes, and add 5% (v / w) of Lactobacillus plantarum CGMCCNO.7184 according to the weight of the solid matrix. Bacterial solution and 95% (v / w) sterile water, mixed evenly, put into a sterile fermentation vessel with a height-to-diameter ratio of 5:1 (bottom sieve plate, high-purity ultra-high molecular weight polyethylene), 35 After culturing at ℃ for 24 hours, wash with 18 times the volume of 0.5% sterile glucose solution (acid protease containing 200U / g protein feed material, amylase 400U / g starch feed material), and collect the effluent in a stirred tank. In the culture tank, culture at 37℃, 30r / min for 2 hours, the number of lactic acid bacteria reached 3.2×10 9 Individuals / mL or more. The solid-state fermentati...

Embodiment 3

[0030] Embodiment 3: the application of the lactic acid bacteria liquid of the present invention in fermented feed

[0031] The preparation method of conventional lactic acid bacteria seed liquid: Pediococcus pentosaceae is inserted into the MRS medium through the activated slope and cultured at 37°C for 36 hours, and then expands the culture step by step with 5% inoculation amount for 24 hours until sufficient solid-state fermentation dosage, such as 0.2L to 4L To 80L to 1600L, for 30 tons of fermented feed; MRS medium (g L -1 ): glucose 20, beef extract 10, tryptone 10, yeast extract 5, K 2 HPO 4 2, triammonium citrate 2, sodium acetate 5, MgSO 4 0.1, MnSO 4 ·H 2 O0.05, Tween-801, pH natural, sterilized at 121°C for 20min.

[0032] Mix 5% (v / m) of the seed liquid prepared by this method and the conventional method and 95% (v / m) of sterile water evenly, add it to the sterile soybean meal, and ferment at 37±5°C for 60 and 72 hours respectively Then dry.

[0033] The bac...

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PUM

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Abstract

The invention discloses a method for continuously preparing lactic acid bacterial liquid for fermented feed, and belongs to the field of animal fermented feed. The method comprises the steps that feedstuff is adopted as main raw materials, stirred evenly, then sterilized and contained in a abacterial fermenting vessel; 5% of the lactic acid bacterial liquid and 95% of abacterial water are added according to the weight of solid-state matrixes, mixed evenly and cultivated at 35 DEG C for 24 h, and then the mixture is washed with 18-20 times 0.5% abacterial glucose solutions containing 200 U / g acid proteases of protein feedstuff and 400 U / g amylases of starch feedstuff; effluent liquid is collected into a cultivating tank with a stirring function to be cultivated at 37 DEG C at 30 r / min for 2-4 h, and the number of lactic acid bacteria can be more than 109 / mL. The solid-state fermentation matrixes are washed once every 16-24 h and totally can be washed for 8 times repeatedly. The method can supply the technical basis to production of lactic acid bacteria seeds of the fermented feed industry such as fermented soybean meal, fermented peanut meal, fermented cotton meal and fermented repeseed meal and is suitable for large-area application and popularization.

Description

technical field [0001] The invention relates to a method for continuously preparing lactic acid bacteria liquid for fermented feed, belonging to the field of animal fermented feed. Background technique [0002] At present, an important factor affecting the development speed and quality of my country's aquaculture industry is the shortage of feed raw materials. The main raw materials of the feed industry are grain raw materials (accounting for about 60%), protein raw materials (accounting for about 30%) and additives. Due to the fact that our country has a lot of people and little land, the unbalanced structure of grain, economy and feed crops has not been effectively resolved for a long time. The supply of raw materials has become the bottleneck of the development of the feed industry, especially the shortage of protein feed raw materials. It is and will be in the future. The most important factor restricting the development of my country's feed industry. [0003] At presen...

Claims

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Application Information

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IPC IPC(8): A23K1/00A23K1/14C12N1/20
CPCY02P60/87
Inventor 陆健蔡国林侯德宝朱德伟
Owner 连云港九洲科技饲料有限公司
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