Chinese herbal medicine micro-ecological compound preparation for preventing and treating enteritis of newborn piglets and preparation method and application thereof

A compound preparation and technology for newborn piglets, applied in application, drug combination, medical formula, etc., can solve the problems of piglets' own immunity decline, pathogenic bacteria resistance to drugs, etc., and achieve the effect of increasing daily weight gain

Active Publication Date: 2021-04-02
JIANGXI AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past, the control measures for piglet diarrhea were mainly the use of antibiotics, but long-term use of antibiotics will lead to drug resistance of pathogenic bacteria, resulting in the decline of piglets' own immunity

Method used

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  • Chinese herbal medicine micro-ecological compound preparation for preventing and treating enteritis of newborn piglets and preparation method and application thereof
  • Chinese herbal medicine micro-ecological compound preparation for preventing and treating enteritis of newborn piglets and preparation method and application thereof
  • Chinese herbal medicine micro-ecological compound preparation for preventing and treating enteritis of newborn piglets and preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The screening of embodiment 1 lactobacillus plantarum R23 and lactobacillus amylovora K2

[0028] 1. Primary screening

[0029] Based on the analysis results of intestinal flora of healthy / diarrhea piglets and the analysis of birth canal flora of healthy / endometritis sows, the dominant genus Lactobacillus was isolated from the birth canal of healthy sows and the intestinal tract of healthy piglets. Collect 10 samples of feces from the birth canal of healthy sows and healthy piglets, and dilute the samples to a suitable gradient with physiological saline, spread them on MRS medium, and cultivate 8 strains of lactic acid bacteria, which are tentatively named: K1, K2, and 12.1 , 12.2, H1, R12, R22, R23.

[0030] Preparation method of MRS liquid medium: peptone 10.0g, beef extract powder 5.0g, yeast extract powder 4.0g, triammonium citrate 2.0g, glucose 20.0g, Tween 80 1.0mL, sodium acetate 5.0g, dipotassium hydrogen phosphate 2.0g, 0.2g magnesium sulfate, 0.05g manganese...

Embodiment 2

[0045] Embodiment 2 preparation of Chinese herbal medicine microecological compound preparation

[0046] 1) Mix 40 parts of Astragalus membranaceus, 30 parts of Shanxiang round leaves, 30 parts of dandelion, 25 parts of Daqingye, 18 parts of Artemisia argyi, five kinds of Chinese herbal medicines, add 3 times the volume of water and boil 3 times, 50 minutes each time, combine and fry 3 times The liquid is boiled and concentrated to a compound Chinese herbal medicine with a crude drug content of 0.5g / mL.

[0047] 2) Add the compound Chinese herbal medicine prepared in step 1) into MRS broth to prepare a culture solution, adjust the pH value to 7.0, sterilize with high-pressure steam at 115°C for 30 minutes, and let it cool to room temperature, add Lactobacillus plantarum R23 and Lactobacillus amylovora K2 was inserted into the above-mentioned culture solution with an inoculum amount of 3%, and fermented at 37° C. for 72 hours to obtain the Chinese herbal medicine microecologica...

Embodiment 3

[0049] 1) Mix 10 parts of Astragalus membranaceus, 30 parts of Shanxiang round leaves, 10 parts of dandelion, 30 parts of Daqingye, 5 parts of Artemisia argyi, five Chinese herbal medicines, add 3 times the volume of water and boil 3 times, 50 minutes each time, combine and fry 3 times The liquid is boiled and concentrated to a compound Chinese herbal medicine concentrate with a crude drug content of 0.5g / mL.

[0050] 2) Add the compound Chinese herbal medicine prepared in step 1) into MRS broth to form a culture solution, adjust the pH value to 7.0, sterilize with high-pressure steam at 115°C for 30 minutes, let it cool to room temperature, and mix the lactobacillus solution in a weight ratio of 1:40 Insert it into the above-mentioned culture solution and ferment for 72 hours at 37° C. to prepare a Chinese herbal medicine microecological compound preparation.

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Abstract

The invention provides a Chinese herbal medicine micro-ecological compound preparation for preventing and treating enteritis of newborn piglets and a preparation method and application thereof, and belongs to the technical fields of veterinary traditional Chinese medicines and feed additives. The Chinese herbal medicine micro-ecological compound preparation is prepared by adding lactobacillus plantarum and lactobacillus amylophilus solution into an extracting solution of Chinese herbal medicines for anaerobic fermentation; the weight ratio of the lactobacillus solution to the Chinese herbal medicines is (1-3): (40-50). The Chinese herbal medicine micro-ecological compound preparation comprise the following Chinese herbal medicines in parts by weight: 2-8 parts of Radix Astragali, 2-6 partsof Folium Turpiniae, 2-6 parts of Herba Taraxaci, 2-6 parts of folium isatidis and 1-5 parts of folium artemisiae argyi. The Chinese herbal medicine micro-ecological compound preparation provided bythe invention can be used for producing pig feed and preventing and treating the enteritis of the newborn piglets. By utilizing the synergistic effect between the lactobacillus and the Chinese herbalmedicines, the Chinese herbal medicine micro-ecological compound preparation has the effects of improving the body immunity, inhibiting harmful bacteria, improving the intestinal microbial environmentand the like. The piglet bacterial enteritis can be effectively prevented and treated.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine for veterinary use and the technical field of feed additives, in particular to a Chinese herbal medicine micro-ecological compound preparation for preventing and treating neonatal piglet enteritis and its preparation method and application. Background technique [0002] In recent years, with the advancement of large-scale pig breeding, the existing problems have become more and more prominent. Better pig disease control, effectively improving the survival rate of piglets, and thus increasing the breeding income have become the research needs of pig farming at this stage. one of the key issues. Diarrhea is one of the common diseases of piglets. It has a wide range of prevalence. It can reduce the daily gain and feed conversion rate of piglets, cause slow growth of piglets, and increase the mortality of piglets. It is the main factor that damages the growth performance of piglet...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/12A23K10/30A23K50/30A61K36/481A61K36/704A61K36/855A61P1/00A61P1/12A61P37/04A61K35/744
CPCA23K10/12A23K10/30A23K50/30A61K36/481A61K36/185A61K36/288A61K36/855A61K36/704A61K36/315A61K36/195A61K36/282A61K35/744A61P1/12A61P1/00A61P37/04A61K2236/19A61K2300/00
Inventor 张锦华吴允正王娇陶绣花
Owner JIANGXI AGRICULTURAL UNIVERSITY
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