Molecular marker linked with cucumis sativus peel gloss regulation gene and application

A technology of molecular markers and gene regulation, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, food processing, etc., can solve problems such as peel shortage, reduce workload, reduce planting scale, improve efficiency and accuracy sexual effect

Active Publication Date: 2021-04-02
TIANJIN RES INST OF VEGETABLE
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Previous studies on the gloss of cucumber peels mostly focused on the presence or absence of gloss, but the research on the strength of peel gloss is still lacking.

Method used

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  • Molecular marker linked with cucumis sativus peel gloss regulation gene and application
  • Molecular marker linked with cucumis sativus peel gloss regulation gene and application
  • Molecular marker linked with cucumis sativus peel gloss regulation gene and application

Examples

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Embodiment 1

[0035] Example 1 Acquisition of Molecular Markers Linked to Regulatory Genes of Cucumber Pericarp Gloss

[0036] 1. GWAS analysis method

[0037] 1. Genome-wide association study (GWAS) is a method for overall association analysis of common genetic variation (single nucleotide polymorphism and copy number) genes in the whole genome. The population is the research object, based on the linkage disequilibrium (LD) between genes (loci) retained after long-term recombination, combining the diversity of target trait phenotypes with the polymorphism of genes (or marker loci) Through combined analysis, genetic loci or marker loci that are closely related to phenotypic variation and have specific functions can be directly identified. Deep resequencing was performed on 289 cucumber germplasms. The average sequencing depth was 15X, and a total of 2,352,638 SNP sites were obtained. After filtering, a total of 399,352 high-quality SNPs were finally obtained, and a comprehensive set of hi...

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Abstract

The invention discloses a molecular marker linked with a cucumis sativus peel gloss regulation gene and application. The molecular marker is mutation from T to C at a 26138107 bp position on a chromosome 3 of cucumis sativus genome. 289 parts of seed resources collected by a project group are utilized, methods of whole genome association analysis (GWAS) combined with QTL positioning and the like are adopted, key variation sites influencing cucumis sativus peel gloss regulation and control are identified, and the molecular marker is developed according to the variation. One KASP molecular marker is designed, the marker is used for carrying out genotype identification on 295 single plants in a segregation population, and coincidence rate reaches 100%. Research results not only are beneficialto early identification and assisted breeding of cucumis sativus with glossy peels, but also provide a basis for map-based cloning of peel gloss regulation and control genes and analysis of molecularmechanisms of gloss regulation and control, and have a wide popularization value.

Description

technical field [0001] The invention belongs to the molecular biology field of cucumber breeding, and relates to a molecular marker linked with a cucumber (cucumis sativus L) pericarp luster regulating gene and the application of the molecular marker in identification and breeding of cucumber pericarp luster. Background technique [0002] Peel gloss is an important commodity appearance trait of cucumber, and it is the focus of cucumber basic research and breeding research. Vanvliet and Meysing's research on the surface gloss of cucumber peels in 1974 showed that the dull (D, Dull fruitskin) gene is a main gene that affects the dark traits of cucumber fruits, and has a relationship with cucumber resistance to downy mildew and powdery mildew. certain correlation. Pierce and Wehner conducted genetic analysis on the dark traits of cucumber pericarp, and believed that the dark traits of cucumber pericarp were monogenic dominant inheritance, and dull versus glossy single gene dom...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/13C12Q2600/156C12Q2600/172C12Q2531/113C12Q2563/107Y02P60/87
Inventor 黄洪宇李愚鹤张利东孔维良李加旺魏爱民王惠哲
Owner TIANJIN RES INST OF VEGETABLE
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