Molecular marker linked with cucumis sativus peel gloss regulation gene and application

Abstract

The invention discloses a molecular marker linked with a cucumis sativus peel gloss regulation gene and application. The molecular marker is mutation from T to C at a 26138107 bp position on a chromosome 3 of cucumis sativus genome. 289 parts of seed resources collected by a project group are utilized, methods of whole genome association analysis (GWAS) combined with QTL positioning and the like are adopted, key variation sites influencing cucumis sativus peel gloss regulation and control are identified, and the molecular marker is developed according to the variation. One KASP molecular marker is designed, the marker is used for carrying out genotype identification on 295 single plants in a segregation population, and coincidence rate reaches 100%. Research results not only are beneficialto early identification and assisted breeding of cucumis sativus with glossy peels, but also provide a basis for map-based cloning of peel gloss regulation and control genes and analysis of molecularmechanisms of gloss regulation and control, and have a wide popularization value.

Description

technical field [0001] The invention belongs to the molecular biology field of cucumber breeding, and relates to a molecular marker linked with a cucumber (cucumis sativus L) pericarp luster regulating gene and the application of the molecular marker in identification and breeding of cucumber pericarp luster. Background technique [0002] Peel gloss is an important commodity appearance trait of cucumber, and it is the focus of cucumber basic research and breeding research. Vanvliet and Meysing's research on the surface gloss of cucumber peels in 1974 showed that the dull (D, Dull fruitskin) gene is a main gene that affects the dark traits of cucumber fruits, and has a relationship with cucumber resistance to downy mildew and powdery mildew. certain correlation. Pierce and Wehner conducted genetic analysis on the dark traits of cucumber pericarp, and believed that the dark traits of cucumber pericarp were monogenic dominant inheritance, and dull versus glossy single gene dom...

AI Technical Summary

Problems solved by technology

Previous studies on the gloss of cucumber peels mostly focused on the presence or absence of gloss, but the research on the strength of peel gloss is still lacking. Therefore, cucumber materials with strong luster, as an important germplasm resource, will be favored by future breeding. Favored by households and market consumers

Images