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Microbial identification method, device and equipment and storage medium

An identification method and microbial technology, applied in the field of microbial identification, can solve the problems of incomplete microbial classification system, errors, and low accuracy.

Pending Publication Date: 2021-04-06
北京携云启源科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the prior art, the types of microorganisms are manually identified by referring to the classification criteria included in the microbial classification system, such as the morphology of a single microbial cell, the utilization of surrounding environmental resources, and serological responses. However, the current microbial classification The system is not complete and there are large errors, so the accuracy rate of the classification standard in the microbial classification system is low, which makes the accuracy rate of microbial identification based on the classification standard also low

Method used

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  • Microbial identification method, device and equipment and storage medium
  • Microbial identification method, device and equipment and storage medium
  • Microbial identification method, device and equipment and storage medium

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0093] figure 1 A flow chart of a microorganism identification method provided in Embodiment 1 of the present application is shown, as figure 1 As shown, the method includes the following steps:

[0094] Step S101: After obtaining the mass spectrum of the microorganism to be identified, according to the intensity value of each spectrum peak in the mass spectrum, determine the area of ​​each of the spectrum peaks in the mass spectrum, wherein each of the intensity values ​​is used for Indicates the content of each protein contained in the microorganism to be identified.

[0095] Specifically, the microorganism to be identified is an unknown microorganism, and in order to determine the type of the unknown microorganism, it is necessary to use the mass spectrum of the unknown microorganism to identify the unknown microorganism, and the abscissa of the mass spectrum represents each protein contained in the microorganism to be identified The mass-to-nucleus ratio, so the mass val...

Embodiment 2

[0153] Figure 5 It shows a schematic structural diagram of a microorganism identification device provided in Example 2 of the present application, as Figure 5 As shown, the above-mentioned microorganism identification device includes:

[0154] The first determination module 501 is used to determine the area of ​​each spectral peak in the mass spectrum according to the intensity value of each spectral peak in the mass spectrum after acquiring the mass spectrum of the microorganism to be identified, wherein each of the The intensity value is used to represent the content of each protein contained in the microorganism to be identified;

[0155] The first calculation module 502 is used for calculating the difference between the mass value of each reference spectral peak in the reference mass spectrum and the mass value of each spectral peak in the mass spectrum for each reference mass spectrum of known microorganisms in the database , wherein, the mass value of each of the spe...

Embodiment 3

[0192] The embodiment of the present application also provides a computer device 600, Image 6 It shows a schematic structural diagram of a computer device provided by Embodiment 3 of the present application, as shown in Image 6 As shown, the device includes a memory 601, a processor 602, and a computer program stored on the memory 601 and operable on the processor 602, wherein the processor 602 implements the above-mentioned microorganism identification method when executing the above-mentioned computer program.

[0193] Specifically, the above-mentioned memory 601 and processor 602 can be general-purpose memory and processor, which are not specifically limited here. When the processor 602 runs the computer program stored in the memory 601, it can execute the above-mentioned microorganism identification method, which is conducive to improving the microorganism identification. the accuracy rate.

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Abstract

The invention provides a microorganism identification method, device and equipment and a storage medium. The microorganism identification method comprises the steps: determining the region of each spectrum peak in a mass spectrum after the mass spectrum of a to-be-identified microorganism is obtained; for each reference mass spectrogram, calculating the difference between the mass value of each reference spectrum peak in the reference mass spectrogram and the mass value of each spectrum peak in the mass spectrogram; for each spectrum peak, determining the number of difference values in a first preset range in the difference values corresponding to the spectrum peak, and taking the number as the matching number of the spectrum peak; calculating a score of the reference mass spectrum; calculating a first probability that the highest score in the scores appears in all the scores; judging whether the first probability is smaller than a preset threshold; and if so, displaying the reference mass spectrogram corresponding to the highest score on a display screen. By means of the method, the accuracy of microbial identification can be improved.

Description

technical field [0001] The present application relates to the technical field of microorganism identification, in particular, to a method, device, equipment and storage medium for microorganism identification. Background technique [0002] With the development of science and technology, microbial identification has become an important part of microbial taxonomy. Through microbial identification, people can understand the whole picture of the microbial world, which is conducive to the further development and utilization of abundant microbial resources. [0003] In the prior art, by referring to various classification standards included in the microbial classification system, such as classification standards such as the morphology of a single microbial cell, the utilization of surrounding environmental resources, and serological responses, the type of microorganisms is manually identified, but the current microbial classification The system is not complete and there are large ...

Claims

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Application Information

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IPC IPC(8): G16B25/10G16B40/10
CPCG16B25/10G16B40/10
Inventor 黄泽炽张鑫磊李红磊孔祥雅
Owner 北京携云启源科技有限公司