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Method for extracting small molecular active peptide from fish skin and application

A small molecule active peptide, fish skin technology, applied in the preparation methods of peptides, chemical instruments and methods, peptides and other directions, can solve the problems of environmental pollution, waste of resources, low utilization rate of fish skin, etc., to activate cell function, improve Effects of Denaturing Temperature, Rapid Fibril-forming Ability

Inactive Publication Date: 2021-04-09
海南省焕颜茹玉生物科技集团有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the utilization rate of fish skin is low, and most of them are discarded in the form of waste, which wastes resources and pollutes the environment. Fish skin cannot be used to make better collagen, so that more collagen peptides can be produced to form small molecular peptide

Method used

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  • Method for extracting small molecular active peptide from fish skin and application

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Embodiment

[0028] A method and application for extracting small molecular active peptides from fish skin, comprising the following steps:

[0029] 1. A method and application of extracting small molecular active peptides from fish skin, comprising the steps of:

[0030] Sp1: Pretreatment of fish skin: the pretreatment of the fish skin includes removing pigments, miscellaneous proteins, lipids and impurities inside the fish skin, and the pretreatment of the fish skin adopts 0.1m / L hydrogen oxidation Sodium immersion for 24 hours to remove pigment and miscellaneous proteins, 10% butanol immersion for 12 hours to degrease lipids, and impurities inside fish skin to be removed by ultrasound for 30min-60min.

[0031] Collagen is extracted from fish skin, and the content and activity of collagen are determined. Compared with mammals, fish skin collagen forms high tensile strength through covalent cross-linking such as hydrogen bonds of glycine triplets, and maintains its structural stability. ...

Embodiment 2

[0041] Small molecule peptides can regulate human immunity and activate cell functions; have a certain hemostatic effect; have a certain relief effect on joints, muscles and bones, etc.; can assist the secretion of breast milk, so it is suitable to be added to milk powder for pregnant women; collagen peptides have cosmetic effects , can develop polypeptide food with beauty effect: after adding collagen polypeptide in food, it can be used as nutrition for diabetics: in addition, collagen polypeptide can also prevent anemia, promote mineral absorption, lose weight and other functions. Collagen contains a variety of bioactive peptides, such as inhibition of angiotensin-converting enzyme activity, inhibition of platelet aggregation, antioxidant activity, and anti-tumor activity.

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Abstract

The invention provides a method for extracting small molecule active peptide from fish skin and application thereof, and relates to the field of small molecule peptide extraction. The method for extracting the small molecule active peptide from the fish skin comprises the following steps of 1, pretreatment of the fish skin; 2, extraction of collagen in the fish skin; 3, separation and purification of fish skin collagen; 4, preparation of collagen polypeptide through hydrolysis; and 5, decomposition of the polypeptide into small molecule peptide. The collagen is extracted from the fish skin and then the small molecule peptide is extracted; the small molecule peptide can adjust the immunocompetence of the human body and activate cell functions, has a certain hemostatic effect, and has a certain relieving effect on pains of joints, muscles and bones and the like; and the collagen polypeptide has a beautifying effect, can be developed into a polypeptide food with the beautifying effect, and also has multiple functions of preventing anemia, promoting mineral absorption, losing weight and the like.

Description

technical field [0001] The invention relates to the field of extraction of small molecule peptides, in particular to a method and application for extracting small molecule active peptides from fish skin. Background technique [0002] Collagen polypeptide is a product of various collagens (animal skin, bone, joints, etc.) after directional gradient hydrolysis with various proteases, and its molecular weight is below 2000Da. Collagen polypeptide can be completely dissolved in water (cold water can also be dissolved), the aqueous solution has low viscosity, and it has fluidity even at a high concentration of 60%. There is no precipitation when heated, and because of its smaller molecular weight, it is easier to be absorbed by the human body. Compared with vegetable protein, collagen polypeptide has a higher absorption and utilization rate. The degree of absorption can reach 100%, there is no allergic reaction, and it can promote the absorption of other proteins in food. Coll...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/78C12P21/06C07K1/30C07K1/16C07K1/14
CPCC07K14/78C12P21/06
Inventor 邵翠霞
Owner 海南省焕颜茹玉生物科技集团有限公司
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