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A Molecular Marker Linked to the Main Effect QTL Site of Peanut Hundred Kernel and Its Application

A technology of molecular markers and all kinds of kernels, which is applied in the field of molecular genetics and crop molecular breeding, can solve the problems of large QTL interval, low contribution rate of molecular marker phenotype, general effect, etc., and achieve the effect of speeding up the breeding process

Active Publication Date: 2022-06-17
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, scholars at home and abroad have some reports on the QTL mapping of peanut weight (Wang et al., 2019; Huang et al., 2015; Fonceka et al., 2012; Li et al., 2011; Ravi et al., 2011 ), but the QTL identified in these studies is usually too sparse due to the marker density, resulting in a large QTL interval identified, and the phenotypic contribution rate of the linked molecular markers is usually low. General effect

Method used

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  • A Molecular Marker Linked to the Main Effect QTL Site of Peanut Hundred Kernel and Its Application
  • A Molecular Marker Linked to the Main Effect QTL Site of Peanut Hundred Kernel and Its Application
  • A Molecular Marker Linked to the Main Effect QTL Site of Peanut Hundred Kernel and Its Application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The present embodiment provides a screening method for the linked molecular marker of the main effect QTL locus of Peanut baicalensis, which specifically includes the following steps:

[0044] 1. A recombinant inbred line (RIL) population was constructed with Zhonghua 16 and sd-H1 as parents. The female parent Zhonghua 16 is a pearl bean type peanut variety with large fruit, high yield and high oil, and the male parent sd-H1 is a multi-grain type of small fruit. For low-yielding peanut varieties, the two parents are quite different in agronomic traits such as grain weight (100-kernel weight), such as figure 1 shown.

[0045]2. Zhonghua 16, sd-H1 and F6 (2016), F7 (2017), F8 (2018) 242 lines of the RIL population were sown in two experimental bases in Wuhan and Yangluo with 3 replicates in a completely randomized block. , Harvest and dry the material after the material matures naturally. According to the standard in "Peanut Germplasm Description Specifications and Data ...

Embodiment 2

[0050] The present embodiment verifies the linkage between two molecular markers related to 100 kernel weight obtained in Example 1 and the traits in peanut varieties, and the details are as follows:

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Abstract

The invention relates to the technical fields of molecular genetics and crop molecular breeding, in particular to a molecular marker linked to a main effect QTL locus of peanut weight and its application. The molecular markers include molecular marker Ah011476, and / or, molecular marker Ah011478; the molecular marker Ah011476 is the 14206230th position on the peanut B06 chromosome, and the polymorphism is G or T; the molecular marker Ah011478 is the 1st position on the peanut B06 chromosome 17650027 bit, polymorphism is C or A. The present invention screens and obtains molecular markers linked to main effect QTL sites of peanut 100-kernel weight. The molecular markers can be used to effectively identify peanut 100-kernel weight and be used for peanut genetic improvement to obtain peanut varieties with high 100-kernel weight.

Description

technical field [0001] The invention relates to the technical field of molecular genetics and crop molecular breeding, in particular to a molecular marker linked with the main QTL site of peanut baicalensis and its application. Background technique [0002] Peanut (Arachis hypogaea L.) is one of the important oil crops and cash crops at present. It has a large planting area and high yield. It can be used for oil extraction and is one of the important sources of edible oil. Peanut is also an important food processing raw material, and food consumption accounts for more than 35% of the total output. At present, increasing the yield per unit area is one of the effective ways to further increase the supply of peanut raw materials. Studies have shown that peanut 100-kernel weight has the greatest impact on yield and is the most important peanut yield component of peanuts. Therefore, it is very important to carry out the QTL mapping study of peanut 100-kernel weight, identify th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/13C12Q2600/156C12Q2600/172C12Q2531/113
Inventor 王志慧廖伯寿雷永晏立英陈玉宁淮东欣王欣康彦平
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI