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Python-based next-generation sequencing sample mixing method, application, equipment and computer readable storage medium

A second-generation sequencing and computer program technology, applied in the field of high-throughput sequencing, can solve the problems of slow mixing volume, error-prone, and inability to mix samples, so as to avoid manual calculation and sorting errors, improve efficiency and accuracy Sexuality, clear and clear output results

Active Publication Date: 2021-04-09
SHANGHAI OE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, in actual production, it is often encountered that samples of different species or samples with different data volumes are mixed in the same flow cell in one machine, and the sample joints in the same flow cell must not be repeated and the total data volume In enough cases, due to the large sample size, manual combination and sorting and calculation of mixed sample volume are slow, error-prone, and there will be a large number of remaining samples that cannot be mixed.

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  • Python-based next-generation sequencing sample mixing method, application, equipment and computer readable storage medium
  • Python-based next-generation sequencing sample mixing method, application, equipment and computer readable storage medium
  • Python-based next-generation sequencing sample mixing method, application, equipment and computer readable storage medium

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Embodiment

[0022] The present invention provides a python-based second-generation sequencing sample mixing method, comprising the following steps: reading and analyzing files, sorting samples into a flow cell, adding joint sequences to samples, calculating sample mixing volume, and outputting a mixing table;

[0023] The file is an excel form in a standard format containing the basic information of the next-generation sequencing sample, and automatically parses multiple forms, and classifies the form data into routine sample data and supplementary test sample data;

[0024] The sample is a nucleic acid sample to be sequenced, which is sequentially filled into the flow cell;

[0025] The flow cell needs to be kept intact; the completeness is to ensure that the joint names of the same flow cell are not repeated; and / or, the total amount of data is not lower than the lower limit and not higher than the upper limit; and / or, the number of supplementary samples does not exceed the upper limit;...

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Abstract

The invention discloses a python-based next-generation sequencing sample mixing method. The method comprises the following steps: reading and analyzing a file, sequencing samples into a circulating tank, adding a joint sequence into the samples, calculating the sample mixing volume, and outputting a sample mixing table. The method provided by the invention can automatically detect whether each circulation groove contains joint repetition or not, calculate the optimal combined sample in the circulation groove and automatically calculate the optimal sample mixing volume, the output result is clear, and the sample mixing efficiency and accuracy in production are improved. The invention also discloses an application of the method in next-generation sequencing sample mixing.

Description

technical field [0001] The invention belongs to the technical field of high-throughput sequencing, and in particular relates to a method for mixing samples of next-generation sequencing based on python. Background technique [0002] Python is an interpretive and object-oriented computer programming language, which is widely used in the field of data analysis due to its simple and clear syntax and rich and powerful libraries. [0003] At present, in actual production, it is often encountered that samples of different species or samples with different data volumes are mixed in the same flow cell in one machine, and the sample joints in the same flow cell must not be repeated and the total data volume If it is enough, due to the large sample size, the speed of manual combination and sorting and calculation of the mixed sample volume is slow, error-prone, and there will be a large number of remaining samples that cannot be mixed. Contents of the invention [0004] In order to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G06F8/30G06F40/18
CPCG06F8/315G06F40/18
Inventor 蔡志强闵运丰李晖徐天生吴学标肖云平史贤俊
Owner SHANGHAI OE BIOTECH CO LTD