Method for producing beta-1,3-glucan by utilizing non-growth coupling characteristic of bacteria
A dextran and coupling technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as shortening the production cycle, achieve the effects of shortening the production cycle, simple process, and reducing the production cycle
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[0040]Example 1
[0041]A method of producing β-1,3-glucan using bacterial non-growth coupling characteristics, including the following steps:
[0042]Take a shovel from the Soil Bacillus ATCC 31749 in the seed medium in seed medium, 3 ° C, 240 r / min oscillatable for 20 hours to obtain activated β-1,3-glucan Strain seed culture solution.
[0043]Among them, the composition of the seed medium is 2% of sucrose; (NH4)2HPO4 0.5%; KH2PO4 0.15%; MGSO4· 7h2O 0.1%; Caco3 0.15%; corn pulp 0.15%; pH regulation to 7.2; 121 ° C sterilization for 20 min.
[0044]S2. Take the secondary seed culture liquid into 20 tons of can to accommodate 16m by 5% inoculation3The fermentation tank of the fermentation medium, the fermentation temperature is 30 ° C, the start pH 7.0, the vent is 0.6 Vm, the stirring speed is 150 r / min, and the incubation is 10 hours, and the fermented pH drops to 5.4 inflection point. Oxygen, oxygen pressure 0.3MP, oxygen 10min; When the pH reached 5.4, the fermentation was stopped; the ...
Example Embodiment
[0053]Example 2
[0054]A method of producing β-1,3-glucan using bacterial non-growth coupling characteristics, including the following steps:
[0055]Take a shovel from the Soil Bacillus ATCC 31749 in the seed medium in seed medium, 3 ° C, 240 r / min oscillatable for 20 hours to obtain activated β-1,3-glucan Strain seed culture solution.
[0056]Among them, the composition of the seed medium is 2% of sucrose; (NH4)2HPO4 0.5%; KH2PO4 0.15%; MGSO4· 7h2O 0.1%; Caco3 0.15%; corn pulp 0.15%; pH regulation to 7.2; 121 ° C sterilization for 20 min.
[0057]S2. Take the secondary seed culture liquid into 20 tons of can to accommodate 16m by 5% inoculation3The fermentation tank of the fermentation medium, fermentation temperature is 30 ° C, start pH 7.0, ventilation amount of 0.45 VVM, stirred with a stirring speed of 180 r / min, before the fermentation pH drops to 5.3m, oxygen, oxygen pressure 0.3MP, pass Oxygen 10min; When the pH reaches 5.4, the fermentation is stopped. The amount of cultured bact...
Example Embodiment
[0066]Example 3
[0067]A method of producing β-1,3-glucan using bacterial non-growth coupling characteristics, including the following steps:
[0068]Take a shovel from the Soil Bacillus ATCC 31749 in the seed medium in seed medium, 3 ° C, 240 r / min oscillatable for 20 hours to obtain activated β-1,3-glucan Strain seed culture solution.
[0069]Among them, the composition of the seed medium is 2% of sucrose; (NH4)2HPO4 0.5%; KH2PO4 0.15%; MGSO4· 7h2O 0.1%; Caco3 0.15%; corn pulp 0.15%; pH regulation to 7.2; 121 ° C sterilization for 20 min.
[0070]S2. Take the secondary seed culture liquid into 20 tons of can to accommodate 16m by 5% inoculation3The fermentation tank of the fermentation medium, the fermentation temperature is 30 ° C, the start pH 7.0, the ventilation amount of 0.35 Vm, the stirring speed is 180 r / min, and the incubation is 10 hours, and the fermented pH drops to 5.4 inflection point. Oxygen, oxygen pressure 0.3MP, oxygen 15min; When the pH reached 5.4, the fermentation wa...
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