Method for producing beta-1,3-glucan by utilizing non-growth coupling characteristic of bacteria

A dextran and coupling technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as shortening the production cycle, achieve the effects of shortening the production cycle, simple process, and reducing the production cycle

Active Publication Date: 2021-04-13
JINAN GUOLI BIOLOGICAL SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The main purpose of the present invention is to provide a method for producing β-1,3-glucan by utilizing the non-growth coupling characteristics of bacteria. The method of culturing and feeding whole-cell tra

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0040]Example 1

[0041]A method of producing β-1,3-glucan using bacterial non-growth coupling characteristics, including the following steps:

[0042]Take a shovel from the Soil Bacillus ATCC 31749 in the seed medium in seed medium, 3 ° C, 240 r / min oscillatable for 20 hours to obtain activated β-1,3-glucan Strain seed culture solution.

[0043]Among them, the composition of the seed medium is 2% of sucrose; (NH4)2HPO4 0.5%; KH2PO4 0.15%; MGSO4· 7h2O 0.1%; Caco3 0.15%; corn pulp 0.15%; pH regulation to 7.2; 121 ° C sterilization for 20 min.

[0044]S2. Take the secondary seed culture liquid into 20 tons of can to accommodate 16m by 5% inoculation3The fermentation tank of the fermentation medium, the fermentation temperature is 30 ° C, the start pH 7.0, the vent is 0.6 Vm, the stirring speed is 150 r / min, and the incubation is 10 hours, and the fermented pH drops to 5.4 inflection point. Oxygen, oxygen pressure 0.3MP, oxygen 10min; When the pH reached 5.4, the fermentation was stopped; the ...

Example Embodiment

[0053]Example 2

[0054]A method of producing β-1,3-glucan using bacterial non-growth coupling characteristics, including the following steps:

[0055]Take a shovel from the Soil Bacillus ATCC 31749 in the seed medium in seed medium, 3 ° C, 240 r / min oscillatable for 20 hours to obtain activated β-1,3-glucan Strain seed culture solution.

[0056]Among them, the composition of the seed medium is 2% of sucrose; (NH4)2HPO4 0.5%; KH2PO4 0.15%; MGSO4· 7h2O 0.1%; Caco3 0.15%; corn pulp 0.15%; pH regulation to 7.2; 121 ° C sterilization for 20 min.

[0057]S2. Take the secondary seed culture liquid into 20 tons of can to accommodate 16m by 5% inoculation3The fermentation tank of the fermentation medium, fermentation temperature is 30 ° C, start pH 7.0, ventilation amount of 0.45 VVM, stirred with a stirring speed of 180 r / min, before the fermentation pH drops to 5.3m, oxygen, oxygen pressure 0.3MP, pass Oxygen 10min; When the pH reaches 5.4, the fermentation is stopped. The amount of cultured bact...

Example Embodiment

[0066]Example 3

[0067]A method of producing β-1,3-glucan using bacterial non-growth coupling characteristics, including the following steps:

[0068]Take a shovel from the Soil Bacillus ATCC 31749 in the seed medium in seed medium, 3 ° C, 240 r / min oscillatable for 20 hours to obtain activated β-1,3-glucan Strain seed culture solution.

[0069]Among them, the composition of the seed medium is 2% of sucrose; (NH4)2HPO4 0.5%; KH2PO4 0.15%; MGSO4· 7h2O 0.1%; Caco3 0.15%; corn pulp 0.15%; pH regulation to 7.2; 121 ° C sterilization for 20 min.

[0070]S2. Take the secondary seed culture liquid into 20 tons of can to accommodate 16m by 5% inoculation3The fermentation tank of the fermentation medium, the fermentation temperature is 30 ° C, the start pH 7.0, the ventilation amount of 0.35 Vm, the stirring speed is 180 r / min, and the incubation is 10 hours, and the fermented pH drops to 5.4 inflection point. Oxygen, oxygen pressure 0.3MP, oxygen 15min; When the pH reached 5.4, the fermentation wa...

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Abstract

The invention relates to the technical field of microbial fermentation, in particular to a method for producing beta-1,3-glucan by utilizing non-growth coupling characteristics of bacteria. The method comprises the following steps: activating a strain capable of producing the beta-1,3-glucan to prepare a seed culture solution; inoculating a fermentation culture medium with the seed culture solution, and performing fermentation culture; after the fermentation is finished, transferring 60-80% of fermentation liquor into a conversion tank; adding a fermentation culture medium into the rest 20-40% of the fermentation liquor to achieve initial fermentation volume, continuously performing secondary fermentation, and performing circulating for 5-6 times; and S4, feeding a sugar solution into the conversion tank for several times, and performing whole-cell conversion. According to the method, through high-density fermentation culture and fed-batch sugar supplementing whole-cell transformation, the yield of the beta-1,3-glucan can be remarkably increased, the production period is shortened, the production cost is reduced, and the defects of an existing fermentation process are overcome.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a method for producing beta-1,3-glucan by utilizing the non-growth coupling property of bacteria. Background technique [0002] β-1,3-glucan is a kind of polysaccharide whose main chain is connected by β-1,3-glucosidic bonds. It is a natural and harmless polymer substance, which usually contains different proportions and sizes due to different sources β-1,2 / β-1,4 / β-1,6-linked branches. [0003] β-1,3-glucan has biological activities such as enhancing immune activity, anti-tumor, anti-oxidation, anti-bacteria, anti-virus, anti-fungal, lowering cholesterol, lowering blood fat, etc., and is a good biological effect regulator. At the same time, it also has the functions of moisturizing, anti-inflammation, anti-aging, anti-wrinkle, anti-dandruff, anti-yellowing, accelerating repair, increasing skin elasticity, etc. It is widely used in many fields such as medicine, foo...

Claims

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Application Information

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IPC IPC(8): C12P19/08C12R1/01
CPCC12P19/08
Inventor 袁建国吉武科高先岭
Owner JINAN GUOLI BIOLOGICAL SCI & TECH
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