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Technology for producing acetaldehyde in one step in microbial fermentation method

A microbial fermentation method, acetaldehyde technology, applied in the direction of microorganism-based methods, microorganisms, biochemical equipment and methods, etc., can solve the problem of high cost of acetaldehyde, and achieve the effect of reducing production costs

Inactive Publication Date: 2010-07-21
NANJING HUAZHOU PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to provide a production process for the one-step production of acetaldehyde by microbial fermentation in view of the defect that the cost of producing acetaldehyde in the prior art is too high

Method used

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  • Technology for producing acetaldehyde in one step in microbial fermentation method
  • Technology for producing acetaldehyde in one step in microbial fermentation method
  • Technology for producing acetaldehyde in one step in microbial fermentation method

Examples

Experimental program
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Effect test

Embodiment 1

[0028] Scrape the slanted seeds and inoculate them into 250mL Erlenmeyer flasks filled with liquid medium, each containing 50mL of medium. Seed medium (g / 100mL) consists of glucose: 4, yeast extract: 0.5, potassium dihydrogen phosphate: 0.1, ammonium sulfate: 0.1, magnesium sulfate: 0.05, agar: 2 (for solid medium, liquid seed medium Not added), pH: 7. After the medium is prepared, it is sterilized at 121°C for 20 minutes, cooled and then inoculated. Place the inoculated Erlenmeyer flask on a constant temperature shaker at 25° C., and vibrate at 220 rpm for 48 hours to obtain liquid seeds.

Embodiment 2

[0030] Configure a high-density fermentation medium (g / L), and its composition is as follows: glucose: 150, yeast extract: 10, peptone: 10, ammonium sulfate: 1, potassium dihydrogen phosphate: 1, magnesium sulfate: 1, pH: 7.

[0031] In a 15L full-automatic fermenter, add the above culture medium according to the filling volume of 70% (v / v), and cool to about 25° C. after steam sterilization. Insert the cultured liquid seeds according to the inoculum amount of 5%, carry out aerobic cultivation at a temperature of 25° C. and a stirring speed of 150 rpm, so that the dissolved oxygen level of the fermentation liquid in the tank fluctuates between 30% and 35%. The residual sugar content was detected every 6 hours. When the residual sugar concentration was lower than 5g / L, feed was carried out. After 2 feeds, the wet weight of the cells reached 14.3g / L. At this time, the tank was selected.

Embodiment 3

[0033] Evenly disperse the wet cells obtained by centrifugation in PBS buffer solution with a concentration of 0.2mol / L and a pH of 7.5 to make a 20g / L bacterial suspension, and add hydrogen peroxide at a volume ratio of 1:6 at 25°C Immediately after 10 minutes of solution treatment, the hydrogen peroxide-containing mixture was inoculated.

[0034]In a 100L fermenter, add a 60% (v / v) liquid volume to the fermentation medium, and its composition (g / L) is: glucose: 100, yeast extract: 10, ammonium sulfate: 1, potassium dihydrogen phosphate : 1, magnesium sulfate: 0.5, pH: 7, steam sterilized and cooled to 30°C. Pasteurized n-dodecane was added in a ratio of 2% (v / v), the stirring speed was 150 rpm, and the mixture was thoroughly mixed for 15 minutes. Insert the above-mentioned cell fluid treated with hydrogen peroxide at an inoculum amount of 5% (v / v), control the temperature at 30°C, and ventilate at 0.2v / v.min. The stirring speed is automatically controlled within 500rpm, and...

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Abstract

The invention belongs to the field of biochemical industry and discloses a technology for producing acetaldehyde in one step in a microbial fermentation method. The invention takes zymomonas mobilis efficiently metabolizing ethanol as the starting strain, the acetaldehyde is produced in one step by the technology of oxygen block and gas stripping fermentation coupling after seed liquid preparation, high-density culture and dezincing treatment respectively, and the yield of the acetaldehyde is more than 55 percent. The invention has the advantages of simple technology, stable yield, cleanness, environment protection and low cost, and is suitable for the large-scale production of the acetaldehyde.

Description

technical field [0001] The invention belongs to the field of biochemical industry, and relates to a production process for one-step production of acetaldehyde by microbial fermentation. Background technique [0002] Acetaldehyde (CH 3 CHO) has active chemical properties and is an important raw material in the organic synthesis industry. It is mainly used to produce acetic acid, butanol, ethyl acetate, pyridine, etc. There are two main approaches to industrialized production of acetaldehyde: (1) ethylene approach: the Wacker method, utilizing PdCl 2 , CuCl 2 As a catalyst, air and ethylene react with water to form acetaldehyde. (2) Ethanol is produced after fermentation of sugary raw materials, and then acetaldehyde is produced by chemical oxidation of ethanol. The raw material source of the former is mainly oil. Affected by oil shortage and rising oil prices, this route gradually loses competitiveness in price, and it is an unsustainable development route. The raw mater...

Claims

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Application Information

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IPC IPC(8): C12P7/24C12R1/01
Inventor 徐强孔国平邢华忠孔新福陶兴标
Owner NANJING HUAZHOU PHARMA
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