The invention relates to the technical field of microbial fermentation, in particular to a method for producing beta-1,3-glucan by utilizing non-growth coupling characteristics of bacteria. The method comprises the following steps: activating a strain capable of producing the beta-1,3-glucan to prepare a seed culture solution; inoculating a fermentation culture medium with the seed culture solution, and performing fermentation culture; after the fermentation is finished, transferring 60-80% of fermentation liquor into a conversion tank; adding a fermentation culture medium into the rest 20-40% of the fermentation liquor to achieve initial fermentation volume, continuously performing secondary fermentation, and performing circulating for 5-6 times; and S4, feeding a sugar solution into the conversion tank for several times, and performing whole-cell conversion. According to the method, through high-density fermentation culture and fed-batch sugar supplementing whole-cell transformation, the yield of the beta-1,3-glucan can be remarkably increased, the production period is shortened, the production cost is reduced, and the defects of an existing fermentation process are overcome.