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Extraction method and application of cell functional active substances of saussurea involucrata

A technology of functional activity and cells, which is applied in the field of extracting functional active substances of Saussurea sauraceae cells, can solve the problems of excessive residual organic solvents, difficulty in removing organic solvents, inactivation or decomposition of functional active ingredients of Saussurea sauvignon L.

Active Publication Date: 2021-04-20
DALIAN PRACTICAL BIOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the prior art, organic solvents are mostly used for the extraction of functional active ingredients. After the extraction is completed, some organic solvents are difficult to remove, resulting in excessive organic solvent residues. Moreover, the active ingredients of Snow Lotus Tianshan are all easily oxidative and decomposed components, and the process of evaporating the organic solvent is also difficult. It will lead to the partial inactivation or decomposition of the functional active ingredients of Saussurea tianshanensis

Method used

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  • Extraction method and application of cell functional active substances of saussurea involucrata
  • Extraction method and application of cell functional active substances of saussurea involucrata

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preparation example Construction

[0044] Preparation of reference substance solution: Accurately weigh 10 mg of rutin reference substance dried at 120°C to constant weight, put it in a 50mL volumetric flask, add an appropriate amount of methanol, heat in a water bath to dissolve, let cool, add methanol to the mark, and shake well. That is (the reference substance solution containing 0.2 mg of anhydrous rutin per mL);

[0045] Preparation of standard curve: Accurately measure 1mL, 2mL, 3mL, 4mL, 5mL, 6mL of the reference substance solution, put them in 25mL volumetric flasks respectively, add water to 6mL each, add 1mL of 5% sodium nitrite solution, shake well, let stand for 6min, Add 1mL of 10% aluminum nitrate, shake well, stand for 6min, add 10mL of sodium hydroxide test solution, add water to the mark, shake well, let stand for 15min, use the corresponding reagent without rutin as a blank, measure the absorbance at 500nm, and The absorbance is the ordinate, the concentration is the abscissa, and the standar...

Embodiment 1

[0051] A. Take fresh Tianshan Snow Lotus cells, wash the Snow Lotus cells twice with 5 times the amount of pure water;

[0052] B. After the snow lotus cells are rinsed, put them into a beaker and add 1 times the amount of pure water, and use a homogenizer to homogenize. The speed of the homogenization method is 18000rpm, the homogenization time is 2min / time, and the homogenization is repeated 3 times:

[0053] C. The homogenized Snow Lotus cell water was filtered with 10 μm, 2 μm, and 0.45 μm filter membranes in sequence, and finally all passed through the 0.45um filter membrane to obtain the Tianshan Snow Lotus cell water extract, which was packaged and stored at -5°C.

[0054] After determination, the extract contains 0.12% of total flavonoids, 0.011% of chlorogenic acid, 0.001% of syringin, 0.013% of 1,5-dicaffeoylquinic acid, and the IC of DPPH free radical scavenging test 50 =10.2 μl / mL, IC of ABTS free radical scavenging assay 50 = 20.3 μL / mL.

Embodiment 2

[0056] A. Take fresh Tianshan Snow Lotus cells and wash the Snow Lotus cells 4 times with 3 times the amount of pure water;

[0057] B. After the snow lotus cells are rinsed, add 2 times the amount of pure water and freeze them in a -20°C refrigerator. After the snow lotus cells are completely frozen, thaw and repeat three times;

[0058] C. The thawed snow lotus cells were filtered with 10 μm, 2 μm, and 0.45 μm filter membranes in sequence, and finally all passed through the 0.45um filter membrane to obtain the Tianshan snow lotus cell water extract, which was packaged and stored at -5°C.

[0059] After determination, the extract contains 0.06% of total flavonoids, 0.006% of chlorogenic acid, 0.001% of syringin, 0.007% of 1,5-dicaffeoylquinic acid, IC of DPPH free radical scavenging test 50 =24.2 μl / mL, IC of ABTS free radical scavenging assay 50 = 38.3 μL / mL.

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Abstract

The invention discloses an extraction method and application of cell functional active substances of saussurea involucrata, and belongs to the technical field of plant functional active component extraction. The extraction method of the cell functional active substances of the saussurea involucrata comprises the following steps of taking fresh saussurea involucrata cells, cleaning the saussurea involucrata cells with pure water, and carrying out vacuum pumping to remove surface moisture; adding pure water into the cleaned saussurea involucrata cells, and carrying out wall breaking treatment to obtain an extracting solution; and filtering the extracting solution by using filter membranes with different pore diameters in sequence to obtain a filtrate, and performing sub-packaging and storage. The extraction method disclosed by the invention is simple to operate, high in extraction efficiency and high in active component yield, and the industrial production efficiency is improved.

Description

technical field [0001] The invention relates to the technical field of extraction of plant functional active ingredients, in particular to a method for extracting functional active substances of Snow Lotus cells in Tianshan Mountains and its application. Background technique [0002] Tianshan snow lotus is a rare and precious Chinese herbal medicine commonly used by the people in the alpine regions of western my country. It is a plant of the family Asteraceae. Mountains, the Pamirs and the Altai Mountains. Snow lotus has a long history of application, especially for medicinal purposes. It mainly contains flavonoids, sesquiterpene lactones, coumarins, organic acids, polysaccharides, etc., and has biological activities such as anti-oxidation, anti-rheumatism, analgesia, regulation of cardiovascular system, and anti-cancer . Due to excessive harvesting and digging in recent years, the number of wild Tianshan snow lotus has been greatly reduced, and its use is restricted. [0...

Claims

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Application Information

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IPC IPC(8): A61K8/99A61Q19/00A61Q17/04A61Q19/08A23L33/105
Inventor 刘汉石金银兵黄利
Owner DALIAN PRACTICAL BIOTECH
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