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Application of LncRNA LOC102555148

A drug and sequence technology, applied in the field of lncRNA, can solve the problems of not eliminating hypoxic apoptosis of BMSCs, high apoptosis of BMSCs, and limited efficacy of transplantation, so as to achieve broad clinical application prospects, promote survival, and improve the efficacy of transplantation Effect

Active Publication Date: 2021-04-20
THE AFFILIATED HOSPITAL OF GUIZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the transplanted BMSCs have high apoptosis in the hypoxic microenvironment of the lesion, which limits the efficacy of transplantation. How to inhibit the hypoxic apoptosis of BMSCs is the key to improve the efficacy of transplantation.
There is no drug to prevent the hypoxic apoptosis of BMSCs

Method used

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  • Application of LncRNA LOC102555148
  • Application of LncRNA LOC102555148
  • Application of LncRNA LOC102555148

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] This embodiment is the application of LncRNA LOC102555148, which is an in vitro experiment. The LncRNA LOC102555148 is used to prepare a drug for inhibiting the hypoxic apoptosis of BMSCs, and the drug includes a plasmid containing the sequence of LncRNA LOC102555148 or a lentiviral expression vector containing the sequence of LncRNA LOC102555148;

[0022] The LncRNA LOC102555148 has the nucleotide sequence of SEQ.ID.No.1 in the sequence listing;

[0023] The LncRNA LOC102555148 is a long non-coding RNA LOC102555148, and the BMSCs are bone marrow mesenchymal stem cells.

[0024] In vitro experiments:

[0025] Test A:

[0026] The primary BMSCs were isolated and cultured, and the LncRNA LOC102555148 of BMSCs was knocked out by CRISPR / Cas9 technology, and then the BMSCs were treated with hypoxia (0% oxygen, 95% nitrogen and 5% carbon dioxide) for 48 hours. The experiment was divided into 3 groups: no knockout LncRNA LOC102555148 (normal oxygen concentration), non-knocko...

Embodiment 2

[0033] This embodiment is the application of Lnc LOC102555148, which is an in vivo experiment. The Lnc LOC102555148 is used to prepare and inhibit the hypoxic apoptosis of BMSCs, and then promote the survival of transplanted BMSCs in the hypoxic environment of the femoral head necrosis area, thereby significantly improving the effect of BMSCs on the femoral head. A drug for the transplantation effect of osteonecrosis, the drug comprising a plasmid of the LncRNA LOC102555148 sequence or a lentiviral expression vector comprising the LncRNA LOC102555148 sequence;

[0034] The LncRNA LOC102555148 has the nucleotide sequence of SEQ.ID.No.1 in the sequence listing;

[0035] Test A:

[0036] Firstly, primary BMSCs were isolated and cultured, and LncRNA LOC102555148 of BMSCs was knocked out by CRISPR / Cas9 technology in vitro, or LncRNA LOC102555148 was overexpressed by lentivirus transfection of BMSCs to overexpress LncRNA LOC102555148, and all BMSCs were labeled with green fluorescent ...

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Abstract

The invention provides application of LncRNA LOC102555148, and the LncRNA LOC102555148 is used for preparing a medicine for inhibiting hypoxia apoptosis of BMSCs so as to improve the transplantation curative effect of the BMSCs. The LncRNA LOC102555148 of the invention plays a role in protecting the hypoxic apoptosis of the BMSCs, and the LncRNA LOC102555148 promotes the survival of the BMSCs in an anoxic environment by inhibiting the hypoxic apoptosis of the BMSCs, so that the transplantation curative effect of the BMSCs is improved. Therefore, the LncRNA LOC102555148 can be used for preparing the medicine for inhibiting the hypoxic apoptosis of the BMSCs, and has a wide clinical application prospect.

Description

technical field [0001] The invention belongs to the technical field of LncRNA, and in particular relates to an application of LncRNA LOC102555148. Background technique [0002] Bone marrow mesenchymal stem cells (BMSCs) have strong regenerative capacity and have been used in the transplantation treatment of various ischemic and hypoxic diseases. However, the transplanted BMSCs have high apoptosis in the hypoxic microenvironment of the lesion area, which limits the efficacy of transplantation. How to inhibit the hypoxic apoptosis of BMSCs is the key to improve the efficacy of transplantation. At present, there is no drug to prevent the hypoxic apoptosis of BMSCs. [0003] Long non-coding RNA (long non-coding RNA, LncRNA) is a class of non-coding RNA molecules with a transcript length between 200nt and 100kb. They can regulate the expression of apoptosis-related genes in a sequence-specific manner, and then intervene in apoptosis. Death and other life activities. The applic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7088A61K35/28A61P19/08
Inventor 张飞彭吾训王强王涛徐凤阳张健董文涛
Owner THE AFFILIATED HOSPITAL OF GUIZHOU MEDICAL UNIV