Male Sterility Gene zmtga10 and Its Application in Creating Male Sterile Lines of Maize

Abstract

The invention discloses male sterility gene ZmTGA10 The gene has the nucleotide sequence shown in SEQ ID NO.1, and the protein encoded by the gene has the amino acid sequence shown in SEQ ID NO.2. The present invention uses CRISPR / Cas9 gene editing technology to mutate the gene in wild-type maize. Although the pollen can be formed normally, the anthers do not crack at all, and found that ZmTGA10 Regulatory functions of genes on male reproductive development in maize. Through offspring screening, mutants without transgenic components can be obtained, and stable sterile lines can be created, which is of great significance for the study of anther development in maize. The present invention also aims at obtaining tga10 Functional molecular markers were designed for male sterile mutants, which have important application value in maize male sterile line breeding, sterile hybrid seed production and molecular marker-assisted selection.

Description

technical field [0001] The invention belongs to the field of plant biotechnology breeding, in particular to male sterility genes ZmTGA10 and its application in creating maize male sterile lines. Background technique [0002] Corn is the largest food crop in my country, with an annual planting area of ​​more than 550 million mu. The healthy development of the corn seed industry has great strategic significance for ensuring national food security. At the same time, the corn seed industry is also the seed industry with the largest market value, the highest degree of commercialization, and the highest technological content in the world, and is a strategic location for global seed industry competition. Compared with the international leading level, my country's corn seed industry still has a huge gap in terms of technological innovation and industrial models. First, due to factors such as the lack of fundamental breakthroughs in the basic research on maize male sterility, it is...

AI Technical Summary

Problems solved by technology

First, due to factors such as the lack of fundamental breakthroughs in the basic research on maize male sterility, it is difficult to protect the intellectual property rights of maize inbred lines. Efficiency is slow

Second, the corn seed production industry is still in a labor-intensive stage relying mainly on manual detasseling, with high costs, huge resource consumption, and difficulty in guaranteeing seed quality

CMS is jointly controlled by mitochondrial genes and nuclear genes. Although it has been used in maize breeding and hybrid production, it has problems such as low resource utilization, single cytoplasm of sterile lines, and susceptibility to diseases.

GMS is controlled solely by nuclear genes, which can overcome CMS defects, but it is difficult to reproduce homozygous sterile lines in large numbers through conventional breeding methods

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