Purification method for pneumocandin B0 with few solid wastes

Abstract

The invention belongs to the technical field of organic matter purification, and relates to a purification method for pneumocandin B0 with few solid wastes. The method comprises the following steps of extraction, resin adsorption separation, spherical silica gel chromatography and vacuum concentration. The purity of the finished pneumocandin B0 prepared by the method can be increased to 99wt% or above, and spherical silica gel can be repeatedly used, so that the output of solid wastes in production can be greatly reduced, and the method is more environment-friendly.

Description

technical field

[0001] The invention relates to the technical field of organic matter purification, in particular to a method for purifying pneumocidine B0 with less solid waste. Background technique

[0002] Neomocontin B0 is an intermediate of caspofungin acetate, which is one of the important raw materials in current biopharmaceutical products. Its production and preparation are mainly obtained through fungal fermentation, synthesis and purification. In the current production process of pneumocidine B0, there are great differences in the fungal fermentation raw materials and processes used, as well as the raw materials and processes used in the later purification operations. Therefore, the quality stability and reliability of the current pneumocidine B0 product are relatively poor, and several are reflected in the poor purity stability of the pneumocidine B0 product, which seriously affects the use quality of the pneumocidine B0 product.

[0003] The preparation method o...