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CHI3L1/PC III detection card as well as preparation method and application thereof

A technology of CHI3L1 and detection card, which is applied in the field of nanomaterials and nanomedicine, and can solve problems such as time-consuming, low sensitivity, and inability to meet rapid detection

Inactive Publication Date: 2021-05-07
XIAMEN INST OF RARE EARTH MATERIALS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, the colloidal gold method has the advantages of simple and fast operation, but low sensitivity and inaccurate quantification; immunoturbidimetric method is more sensitive and accurate, and can be applied to automatic biochemical analyzers, but requires instruments and takes a long time, and is suitable for processing a large number of samples. It cannot meet the purpose of rapid detection; the immunofluorescence method is to covalently bind the antibody to the surface active group of the fluorescent microsphere, and judge the result by whether the detection line produces fluorescence after excitation. It is fast, convenient, accurate and quantitative, and has high sensitivity and marker Stable and other advantages, widely used in the field of medical immunoassay

Method used

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  • CHI3L1/PC III detection card as well as preparation method and application thereof
  • CHI3L1/PC III detection card as well as preparation method and application thereof
  • CHI3L1/PC III detection card as well as preparation method and application thereof

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preparation example Construction

[0070] The present invention also provides a method for preparing a CHI3L1 / PCⅢ detection card, comprising the following steps:

[0071] Step 1: preparing activated rare earth nanoprobes according to the preparation method of claim 3;

[0072] Step 2: Prepare PCⅢ antibody 1, CHI3L1 antibody 1, and rabbit IgG antibody labeled with rare earth nanoprobes: add PCⅢ antibody 1, CHI3L1 antibody 1, and rabbit IgG antibody to the activated rare earth nanoprobe in step 1 at 50-200 μg / 200 μl, After blocking with blocking solution, centrifuge at high speed, wash and resuspend with containing preservative solution, and store at 4°C in the dark;

[0073] Step 3: Prepare coating membrane: respectively use CHI3L1 antibody 2, PCⅢ antibody 2 and goat anti-rabbit IgG antibody as the detection line T1, T2 line and quality control line in parallel on the nitrocellulose membrane for coating and drying;

[0074] Step 4: Prepare the bonding pad: Soak the bonding pad in 20mM Tris-HCl with pH 8.0 conta...

Embodiment 1

[0090] The rare earth nanoprobe of this embodiment is sodium yttrium fluoride coated with sodium erbium fluoride with a core-shell structure, and has a particle size of 20nm to 30nm. Its composition is:

[0091] NaErF 4 @NaYF 4 , where NaErF 4 It is an all-erbium-doped core structure; NaYF 4 is the shell, @ means NaYF 4 Coated in NaErF 4 surface.

[0092] Further, the rare earth nanoprobe is stable in the ground state, and emits three emission fluorescences in the wavelength ranges of 500-550nm, 640-680nm and 1500-1600nm under the action of an excitation light source of 808nm. NaErF 4 @NaYF 4 Fluorescence spectra of rare earth nanoprobes excited at 808nm, such as figure 2 shown. NaErF 4 @NaYF 4 TEM images of rare earth nanoprobes, such as image 3 shown.

[0093] The method for preparing the rare earth nanoprobe of this embodiment comprises the following steps:

[0094] Step 1: Synthesis of erbium sodium fluoride core structure: In the container, add oleic acid ...

Embodiment 2

[0098] Such as figure 1 As shown, the CHI3L1 / PCⅢ detection card of the present embodiment includes:

[0099] Substrate 1; as the substrate of the CHI3L1 / PCⅢ detection card;

[0100] Coating film 4; arranged in the middle of the upper surface of the substrate 1;

[0101] Bonding pad 3: overlapped at one end of the upper surface of the coating film 4

[0102] Sample pad 2; overlapped with one end of the upper surface of the binding pad 3;

[0103] Absorbent paper 4; overlapped on the other end of the upper surface of the coating film 3;

[0104] Wherein, the binding pad 3 is spray-coated with microsphere threads 31, and the microsphere threads 31 are PCⅢ, CHI3L1, and rabbit IgG antibodies labeled with rare earth nanoprobes provided in Example 1; Line T1 41, detection line T2 42 and quality control line 43, the detection line 41 is close to the binding pad 3, the detection line T141, detection line T242 and quality control line 43 are parallel to each other, and the distance ...

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Abstract

The invention discloses a CHI3L1 / PC III detection card and a preparation method and application thereof, and belongs to the field of nano materials and nano medicine, an adopted rare earth nano probe is sodium yttrium fluoride coated with erbium sodium fluoride of a core-shell structure, the particle size ranges from 20 nm to 30 nm, the rare earth nano probe is composed of NaErF4 @ NaYF4, NaErF4 is of a total erbium-doped core structure, NaYF4 is a shell layer, and @ indicates that the NaYF4 is coated on the surface of the NaErF4. The nano-probe is a rare earth fluoride nano-material and has the advantages of low background, long luminescence life, strong fluorescence signal, high signal-to-noise ratio and the like, the probe is connected with the antibody through a covalent bond, the labeled product is stable, and the nano-probe has the characteristics of high sensitivity, high accuracy, rapidness, simplicity and convenience in detection and the like, and can assist doctors in judging whether a patient is subjected to hepatic fibrosis or not in the early stage.

Description

technical field [0001] The invention belongs to the field of nanomaterials and nanomedicine, in particular to a CHI3L1 / PCⅢ detection card, its preparation method and application. Background technique [0002] Liver cancer (HCC) is one of the most common malignant tumors and the third leading cause of cancer-related deaths worldwide. Its incidence is second only to lung cancer in my country, ranking second. About 330,000 people die of liver cancer every year in my country, with a high mortality rate. More than 70% of people with liver cancer have liver cirrhosis, and about 50% of patients with liver cirrhosis can progress to liver cancer. Liver cirrhosis caused by liver fibrosis is defined as the late stage of liver fibrosis. The development from liver fibrosis to liver cirrhosis is a continuous dynamic development process, and liver fibrosis and early liver cirrhosis are reversible. Therefore, early detection of liver cirrhosis Fibrosis and timely treatment to control hep...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/558G01N33/551G01N33/543
CPCG01N33/54346G01N33/551G01N33/558G01N33/57438G01N33/57476G01N33/57488
Inventor 张云张肖刘宁宋良
Owner XIAMEN INST OF RARE EARTH MATERIALS
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