Method for cultivating new variety of normal-development intermuscular-bone-free fish

Abstract

The invention relates to a method for cultivating a new variety of normal-development intermuscular-bone-free fish, and relates to a method for cultivating a new variety of intermuscular-bone-free fish. The invention provides the method for removing intermuscular bones under the condition that normal growth of fishes is not influenced. The method for cultivating the new variety of the normal-development intermuscular-bone-free fish comprises the following steps of 1, knocking out a bmp6 gene in a fertilized egg of an original fish variety to obtain an F0-generation individual; 2, performing mutant screening on the F0 generation, and matching to obtain an F1 generation population; 3, performing mutant screening on the F1 generation population, selecting individuals with the same frameshift mutation for mating, constructing an F2 generation, and screening out homozygous mutation individuals in the F2 generation; and 4, propagating the F2-generation homozygous mutation individuals to obtain the new variety of the intermuscular-bone-free fish. According to the method, a strain with intermuscular bones deleted can be obtained, growth is not affected, stable inheritance can be achieved, and the problems that the intermuscular bones are small, difficult to find and difficult to remove in production can be solved.

Description

technical field [0001] The invention relates to a method for cultivating a new species of stickleback without muscles. Background technique [0002] According to the report of the World Food and Agriculture Organization, 90% of the global fish farming output comes from 27 fish species, and 12 of these 27 fish species contain intermuscular spines, and the farming output accounts for more than 69% of the fish farming output. The intermuscular spine is a unique skeleton of teleosts, a small bone located between the muscle segments, which is ossified from tendons or ligaments. Because of the existence of intermuscular spines, it will cause inconvenience and even physical harm to people during eating, and it will also cause troubles in the processing of fish products. Therefore, the genetic improvement of intermuscular spines has become one of the important target traits of fish genetics and breeding. . [0003] At present, there are methods for preparing strains with few inter...

AI Technical Summary

Problems solved by technology

Gao Zexia et al. found that by knocking out the scxa gene in zebrafish, a strain with more than 70% reduction in the number of intermuscular spines can be obtained (invention patent application number: 201911104496.6; Nie et al., 2020), but according to the research, the intermuscular The zebrafish line with reduced number of spines has abnormal development of main axis bones such as ribs, which affects the normal growth of the fish (Nie et al., 2020; Kague et al., 2019)

Although there are currently methods to reduce the number of intermuscular spines by using gene editing technology, they all have some defects, which affect the normal growth of fish

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