Construction method of laying hen intestinal enteritis model

A construction method and technology of laying hens, applied in the field of construction of laying hen enteritis model, can solve problems such as the decline in production performance of laying hens, lack of enteritis models of laying hens, etc.

Active Publication Date: 2021-05-28
SICHUAN AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In view of this, the present invention aims at the lack of enteritis model of laying hens, and provides a method for constructing enteritis model of layin...

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  • Construction method of laying hen intestinal enteritis model
  • Construction method of laying hen intestinal enteritis model
  • Construction method of laying hen intestinal enteritis model

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Embodiment 1

[0029] A method for constructing a laying hen enteritis model, comprising the following steps:

[0030] Step 1. Select 50 Roman pink laying hens during the peak egg production period (28 weeks of age), adopt a single factor experimental design, set up treatment, each treatment has 10 repetitions, and each repetition is 1 chicken, and 10 mL of normal saline is administered to the stomach respectively (control group, CON) and four 10mL challenge treatment groups ( figure 1 ).

[0031] Wherein, the specific operation method of the challenge treatment group is, coccidia vaccine (200 times weak toxicity coccidia quadrivalent vaccine, containing 220,000 coccidia spores) + different gradients of Clostridium perfringens type A treatment group, The doses of different gradients of Clostridium perfringens type A were: 1mL (T1), 2mL (T2), 3mL bacterial liquid (T3) and 4mL bacterial liquid (T4). The test period is 7 days. After the start of the test, 200 times of coccidiosis vaccine (10 ...

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Abstract

The invention discloses a construction method of a laying hen intestinal enteritis model. The construction method comprises the following steps: 40 Roman pink laying hens in the egg laying peak period are selected, and the selected Roman pink laying hens are set as a control group and four challenge treatment groups respectively; the challenge treatment groups are treatment groups of coccidiosis vaccine and A-type clostridium perfringens with different gradients, the coccidiosis vaccine is firstly fed at 9:00 in the morning every day after the test is started, and clostridium perfringens liquid is fed after half an hour; sampling is carried out 48 hours after the last time of feeding; and the test period is 7 days, feed is corn-soybean meal type basic feed, and feeding management is carried out according to conventional feeding management. According to the method, the specific mechanism of reduction of the production performance of the laying hens caused by factors such as nutrition and feed factors, stress and the like can be explored.

Description

technical field [0001] The invention belongs to the technical field of animal nutrition and toxicology, and in particular relates to a method for constructing a laying hen enteritis model. Background technique [0002] The small intestine of animals is an important part of the digestion and absorption of nutrients, and it is also the largest immune organ of the body. A large number of studies have shown that intestinal diseases in chickens will affect the digestion and absorption of nutrients, resulting in a decline in production performance and feed conversion efficiency, which will seriously lead to the death of chickens and seriously restrict the development of the poultry industry. Chicken enteritis is one of the most prominent enteric diseases in the intensive production of modern poultry, which has brought huge economic losses to the poultry industry. Available data show that the economic losses caused by enteritis problems in production to the poultry industry increa...

Claims

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Application Information

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IPC IPC(8): A01K67/02A23K50/75A23K10/30A23K10/37A23K20/158A23K20/20A23K20/26A23K20/142A23K20/174A23K20/105
CPCA01K67/02A23K50/75A23K10/30A23K10/37A23K20/158A23K20/20A23K20/26A23K20/142A23K20/174A23K20/105Y02P60/87
Inventor 王建萍张红叶晏磊毛湘冰尹华东白世平张克英
Owner SICHUAN AGRI UNIV
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