A method for establishing a model of oxidative stress in the ovary of laying hens

Abstract

The invention discloses a method for establishing an ovarian oxidative stress model for laying hens, comprising the following steps: selecting Roman powder laying hens in the peak egg production period, adopting a single-factor experimental design, and setting 6 treatments, which are respectively a control group and a control group. 5 tert-butyl hydroperoxide groups, the control group was fed with physiological saline; sampling was carried out 48 hours after the last feeding; the experimental period was 24 days, the diet was corn-soybean meal type basal diet, and the feeding management was carried out according to conventional feeding management. The egg production rate was recorded, and the concentration of estradiol, etc. was determined by ELISA; the ovaries were collected 48 hours after the last feeding of normal saline and tert-butyl hydroperoxide. After HE staining, the number of primordial follicles was counted, and the pre-grade and grade follicle count and measure; use HPLC and kit method to determine the content or activity of vitamin C in ovary. The method of the invention can increase the content of ovarian free radicals, reduce the activity of ovarian antioxidant enzymes, and cause oxidative stress in laying hens.

Description

technical field [0001] The invention belongs to the technical field of animal reproduction and toxicology, and in particular relates to a method for establishing an ovarian oxidative stress model of laying hens. Background technique [0002] The poultry ovary is a classic model for studying ovarian biology, follicle development and ovarian cancer. The egg-laying performance of poultry is mainly determined by the level of growth and development of follicles in the ovary, and is influenced by factors such as genetics, nutrition and environmental conditions (time and intensity of light). With the advancement of technology in the field of genetics and nutrition, the production performance of commercial laying hens has continued to improve. At present, high-yielding laying hens can lay 410 to 430 eggs in 110 weeks, but the average egg production rate in the whole period (20-110 weeks old) is only 65%. ~70%, still has a certain production potential to be tapped. In actual produc...

AI Technical Summary

Problems solved by technology

A large number of studies have shown that a certain amount of ROS in the follicle has an important physiological effect on its development and ovulation. However, when ROS is excessively increased, it will cause oxidative stress and induce follicular atresia.

Studies on mammals have found that oxidative stress can reduce the number of ovarian follicles at various stages, but the specific mechanism by which oxidative stress inhibits follicular development and induces follicular atresia is unclear. The only studies are mainly in model animals such as humans and mice. On the other hand, very little research has been done on poultry

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