A method for constructing a layer enteroenteritis model

A construction method and technology for laying hens are applied in the field of constructing a model of enteroenteritis of laying hens, which can solve the problems of decreased production performance of laying hens, lack of models of enteroenteritis of laying hens, etc.

Active Publication Date: 2022-04-05
SICHUAN AGRI UNIV
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Problems solved by technology

[0004] In view of this, the present invention aims at the lack of enteritis model of laying hens, and provides a method for constructing enteritis model of laying hens, which can explore the specific mechanism of the decline in production performance of laying hens caused by factors such as nutrition and feed factors and stress

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  • A method for constructing a layer enteroenteritis model
  • A method for constructing a layer enteroenteritis model
  • A method for constructing a layer enteroenteritis model

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Embodiment 1

[0029] A method for constructing a laying hen enteritis model, comprising the following steps:

[0030] Step 1. Select 50 Roman pink laying hens during the peak egg production period (28 weeks of age), adopt a single factor experimental design, set up treatment, each treatment has 10 repetitions, and each repetition is 1 chicken, and 10 mL of normal saline is administered to the stomach respectively (control group, CON) and four 10mL challenge treatment groups ( figure 1 ).

[0031] Wherein, the specific operation method of the challenge treatment group is, coccidia vaccine (200 times weak toxicity coccidia quadrivalent vaccine, containing 220,000 coccidia spores) + different gradients of Clostridium perfringens type A treatment group, The doses of different gradients of Clostridium perfringens type A were: 1mL (T1), 2mL (T2), 3mL bacterial liquid (T3) and 4mL bacterial liquid (T4). The test period is 7 days. After the start of the test, 200 times of coccidiosis vaccine (10 ...

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Abstract

The invention discloses a method for constructing an enteritis model of laying hens, comprising the following steps: selecting 40 Roman pink laying hens during the peak laying period, and setting up treatments, which are respectively a control group and four challenge treatment groups; the challenge treatment group Coccidia vaccine + different gradients of Clostridium perfringens type A treatment group, respectively, at 9:00 every morning after the start of the test, the coccidia vaccine was given first, and then the Clostridium perfringens bacteria solution was given half an hour later. Sampling was carried out 48 hours after the last feeding; the test period was 7 days, the diet was corn-soybean meal-based diet, and the feeding and management were carried out according to conventional feeding and management. The invention can explore the specific mechanism of the decline in production performance of laying hens caused by nutrition and feed factors, stress and other factors.

Description

technical field [0001] The invention belongs to the technical field of animal nutrition and toxicology, and in particular relates to a method for constructing a laying hen enteritis model. Background technique [0002] The small intestine of animals is an important part of the digestion and absorption of nutrients, and it is also the largest immune organ of the body. A large number of studies have shown that intestinal diseases in chickens will affect the digestion and absorption of nutrients, resulting in a decline in production performance and feed conversion efficiency, which will seriously lead to the death of chickens and seriously restrict the development of the poultry industry. Chicken enteritis is one of the most prominent enteric diseases in the intensive production of modern poultry, which has brought huge economic losses to the poultry industry. Available data show that the economic losses caused by enteritis problems in production to the poultry industry increa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/02A23K50/75A23K10/30A23K10/37A23K20/158A23K20/20A23K20/26A23K20/142A23K20/174A23K20/105
CPCA01K67/02A23K50/75A23K10/30A23K10/37A23K20/158A23K20/20A23K20/26A23K20/142A23K20/174A23K20/105Y02P60/87
Inventor 王建萍张红叶晏磊毛湘冰尹华东白世平张克英
Owner SICHUAN AGRI UNIV
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