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Inverse opal membrane for inhibiting scar generation and preparation method thereof

An inverse protein, stone film technology, applied in the direction of peptide/protein components, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc. The effect of accelerating wound healing and repair and promoting cell directional growth

Active Publication Date: 2021-05-28
THE FIRST AFFILIATED HOSPITAL OF WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these membranes are often used simply for cell induction, and their effects on cell growth and migration, especially in the application of inhibiting the scarring process, have not been investigated.

Method used

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  • Inverse opal membrane for inhibiting scar generation and preparation method thereof
  • Inverse opal membrane for inhibiting scar generation and preparation method thereof
  • Inverse opal membrane for inhibiting scar generation and preparation method thereof

Examples

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preparation example Construction

[0032] The invention provides a method for preparing an inverse opal film for suppressing scar generation, comprising the steps of:

[0033] 1) Preparation of inverse opal film: using negative replication method to prepare inverse opal film using silica particles and polylactic acid-glycolic acid copolymer;

[0034] 2) Preparation of an inverse opal film that inhibits scar formation: use the inverse opal film obtained in step 1) to uniformly stretch it and then dry it to obtain a stretched inverse opal film and load methyl groups in the stretched nanopores (figure). Acrylic anhydride gelatin, antimicrobial peptide and vascular endothelial growth factor blend.

[0035] Further, the specific preparation steps of the negative replication method described in step 1) are:

[0036] ①Using silica particles to prepare colloidal crystal templates;

[0037] ② Use the polylactic acid-glycolic acid copolymer solution to infiltrate the interior of the colloidal crystal template, and obta...

Embodiment 1

[0047] A stretched inverse opal film, the preparation process is as follows figure 1 As shown, the steps are as follows:

[0048] (1) Preparation of silica colloidal crystal template:

[0049] Select silicon dioxide particles with a diameter of 400 nm, suspend them in absolute ethanol, vortex and ultrasonically disperse them fully in absolute ethanol, and prepare a silicon dioxide solution with a concentration of 2%. Insert the glass sheet vertically into the silica solution, and place it in a constant temperature and humidity box, and adjust the temperature in the box to 30 degrees Celsius and the humidity to 40%. With the evaporation of absolute ethanol, the silica particles are neatly arranged on the surface of the glass sheet, forming a silica colloidal crystal template.

[0050] (2) Preparation of inverse opal film

[0051]Prime Minister, configure 15% polylactic acid-glycolic acid copolymer ethyl acetate solution, seal and place away from light. Place a foam box in a...

Embodiment 2

[0055] Stretched inverse opal membranes are used to guide cell directional growth and alignment:

[0056] Get the unstretched inverse opal film prepared in Example 1, the inverse opal film stretched three times the original length, the inverse opal film stretched six times the original length and the glass cell climbing sheet, soaked in the ethanol solution of 75% concentration , placed under ultraviolet light for 1 hour, washed repeatedly with sterile PBS buffer for 3 times, and spread in six-well plates respectively, adding 2ml of mouse embryonic fibroblast cell line NIH-3T3 cell suspension (4* 10 4 cells / ml), cultivated for 48 hours at a temperature of 37 degrees centigrade and a carbon dioxide concentration of 5%. Repeatedly wash 2 times with sterile PBS buffer to remove dead cells, stain the cells with 2uM calcein solution, absorb excess dye after 30 minutes, wash once with sterile PBS buffer and observe NIH-3T3 cells with a fluorescent inverted microscope Morphology an...

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Abstract

The invention discloses an inverse opal membrane for inhibiting scar generation and a preparation method thereof, and the method comprises the following steps: preparing the inverse opal membrane with a regular and mutually communicated porous structure by utilizing silicon dioxide particles and a polylactic acid-glycolic acid copolymer through a negative replication method; stretching at the glass transition temperature of a polylactic acid-glycolic acid copolymer to obtain a stretched inverse opal film with an adjustable pattern, and loading methacrylic anhydride gelatin and antibacterial peptide through a vacuum negative pressure method. The prepared inverse opal membrane has good biocompatibility, has the function of guiding directional growth and tidy arrangement of cells and inhibits scar formation of a wound part, the antibacterial peptide loaded in the porous structure can be slowly released to achieve a powerful antibacterial function, so that wound healing is accelerated, and the inverse opal membrane is diversified in function and simple to prepare.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to an inverse opal film for inhibiting scar formation and a preparation method thereof. Background technique [0002] Scar tissue formation often occurs after severe burns, skin trauma, or as a complication following surgical intervention. Hypertrophic scars are mainly caused by persistent dermal fibrosis, irregular collagen deposition, and hyperproliferative hypertrophic scar fibroblasts. The formation of scar tissue is not only aesthetically pleasing, but excess scar tissue can also affect joint mobility. Conventional treatments for hypertrophic scars can be divided into non-invasive treatments (such as topical silicone sheets and pressure dressings) and invasive treatments (such as surgical excision and laser ablation). However, these treatment methods have adverse reactions such as low curative effect, high recurrence rate, and painful process. How to effectively...

Claims

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Application Information

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IPC IPC(8): A61K38/02A61K47/42A61K47/24A61K47/04A61K9/70A61P17/02A61P31/04
CPCA61K38/02A61K47/42A61K47/24A61K47/02A61K9/7007A61P17/02A61P31/04
Inventor 赵远锦翁万青王月桐施可庆张纯武池俊杰
Owner THE FIRST AFFILIATED HOSPITAL OF WENZHOU MEDICAL UNIV
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