Flexible electrochemical biosensor and construction method thereof
A biosensor and construction method technology, applied in the field of flexible electrochemical biosensor and its construction, can solve the problems of electrochemical sensor stability, durability and flexibility, parts peeling and delamination, large difference in modulus, etc.
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Embodiment 1
[0046] Preparation of (sandwich structure) PPy composite film
[0047] Principle: Continue to synthesize 2-naphthalenesulfonate (2-NS) doped PPy on the basis of PEE-doped PPy film, and finally obtain a PPy composite film with a sandwich structure (see figure 1 ).
[0048] The synthesis steps are as follows:
[0049] 1) In an organic phase system, a PPy film doped with PEE was synthesized by electrochemical polymerization, referred to as a PEE-PPy film.
[0050] Specifically: mix isopropanol (pure isopropanol solution) and boron trifluoride ether (47.0-47.7% mass fraction based on boron trifluoride) solution at a volume ratio of 7:3, and then add For 5% PEE, finally add 0.05M pyrrole monomer to prepare organic phase electrolyte.
[0051] Chenhua Electrochemical Workstation 660E was used to synthesize PEE-PPy film by electrochemical polymerization using a three-electrode system, wherein the working electrode was a glass sheet sputtered with titanium and platinum, and the coun...
Embodiment 2
[0057] Building a self-supporting electrochemical biosensor
[0058] (1) Put the synthesized PPy composite film in 0.5mM chloroauric acid solution (containing 0.5M KNO 3 ) is deposited at a voltage of -0.4V for 400s, and the surface of the thin film electrode will be evenly covered with a layer of gold nanoparticles.
[0059] (2) Soak the thin film electrode deposited with gold nanoparticles in the CEA aptamer (the sequence of the aptamer: 5'-SH-ATACCA GCT TAT TCA ATT-3') solution overnight (12-14 hours), pass The role of self-assembly links the CEA aptamer and gold nanoparticles to the surface of the thin film electrode.
[0060] Finally, MCH was used to block the remaining activation sites to ensure the recognition ability of the constructed aptasensor.
Embodiment 3
[0062] Optimization of Experimental Conditions for Flexible Electrochemical Biosensors
[0063] To detect CEA, use non-Faradaic impedance for detection, and the detection method is as follows:
[0064] The constructed biological aptamer film electrode was directly used as the working electrode, connected to the electrochemical workstation, and the saturated calomel electrode and the platinum wire were used as the reference electrode and the counter electrode, respectively, and detected in PBS (0.2M, pH 7.4) solution CEA (10 - 6 g / mL).
[0065] the result shows:
[0066] For CEA exhibits a distinct impedance change response, as figure 2 As shown in CEA / MCH / Aptamer / Au / PPy, the other controls are: PPy, Au / PPy-PPy composite film covered with a layer of gold nanoparticles, Aptamer / Au / PPy combined with aptamer film electrode, MCH / PPy- Aptamer / Au / PPy-blocked aptamer sensor.
[0067] The experimental conditions for the construction of biological aptamer sensors (including the d...
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