Method for regulating proliferation and differentiation of inner ear stem cells by Hippo and application of method in hair cell regeneration

An inner ear stem cell, proliferation and differentiation technology, which is applied in biochemical equipment and methods, microbial determination/inspection, preparations for in vivo experiments, etc., can solve the problems of low sorting efficiency and difficulty in obtaining inner ear stem cells, etc. Separating accurate effects

Pending Publication Date: 2021-06-01
SOUTHEAST UNIV
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Problems solved by technology

[0009] In order to solve the deficiencies mentioned in the above-mentioned background technology, the object of the present invention is to provide a method for Hippo to regulate the proliferation and

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  • Method for regulating proliferation and differentiation of inner ear stem cells by Hippo and application of method in hair cell regeneration
  • Method for regulating proliferation and differentiation of inner ear stem cells by Hippo and application of method in hair cell regeneration
  • Method for regulating proliferation and differentiation of inner ear stem cells by Hippo and application of method in hair cell regeneration

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Embodiment Construction

[0030] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

[0031] The present invention will study its effect on the regeneration of inner ear hair cells from the aspect of Hippo signaling pathway regulation. First, Lgr5-positive inner ear stem cells were sorted out by flow cytometry from the single-cell suspension prepared by digesting the cochlear basement membrane of Lgr5-EGFP-CreERT2 mice, and then the Hippo cells were up-regulated or down-regulated by cell transfection technology. The expression level of YAP, a key fa...

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Abstract

The invention relates to the technical field of biology, in particular to a method for regulating proliferation and differentiation of inner ear stem cells by Hippo and application of the method in hair cell regeneration. The invention discloses a method for regulating proliferation and differentiation of inner ear stem cells by Hippo. The method comprises the following steps: 1, detecting the expression condition of a Hippo signal channel member in a cochlea in a wild mouse; 2, researching a regulation and control mechanism of the Hippo signal on proliferation and differentiation of the inner ear stem cells which are separated in a flow manner in an in-vitro experiment; 3, researching a coordinated regulation mechanism of Hippo and Wnt signals on the inner ear stem cells in an in-vitro experiment, and the like. By using the Lgr5-EGFP-CreERT2 tool mouse, the separation of the inner ear stem cells (Lgr5 positive cells) is accurate and a large amount of the inner ear stem cells (Lgr5 positive cells) can be realized, and a relatively good research premise can be achieved: the Lgr5 positive inner ear stem cells of the mouse are sorted. In addition, in an in-vitro experiment, after the Hippo signal channel is researched to carry out balling and differentiation experiments on the sorted inner ear stem cells, the specific action mechanism of the Hippo signal channel in regulation and control of proliferation/differentiation of the inner ear stem cells and hair cell regeneration can be deeply explored.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for regulating the proliferation and differentiation of inner ear stem cells by Hippo and its application in hair cell regeneration. Background technique [0002] Hearing is one of the most important perceptions of human beings and the basis of language communication. As the basis of language recognition and understanding, auditory perception plays an important role in human social activities. Due to the rapid development of social economy and industrialization, the increasing aging of the population, the abuse of ototoxic drugs and environmental pollution factors such as noise, the number of people suffering from different degrees of ear diseases is increasing year by year. According to WHO statistics in 2019, about 5% of the world, or 466 million people, suffer from disabling hearing loss, of which 34 million are children. [0003] Hearing loss and deafness have become a...

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Application Information

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IPC IPC(8): A61K49/00G01N33/68C12Q1/686
CPCA61K49/0008G01N33/68C12Q1/686G01N2333/47C12Q2521/107
Inventor 柴人杰张莎莎马翔宇程诚高下
Owner SOUTHEAST UNIV
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