Chlorella pyrenoidosa antioxidant peptide and preparation method thereof

A technology of chlorella pyrenoidosa and antioxidant peptides, which is applied to the preparation methods of peptides, chemical instruments and methods, algae/bryopeptides, etc., can solve the problems of low protein extraction rate, low purity, and complicated preparation methods, and achieve The effect of high protein extraction rate, low salt content and high purity

Active Publication Date: 2021-06-01
DONGGUAN UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The antioxidant peptide of chlorella pyrenoidosa is a natural antioxidant. At present, the utilization rate of protein in my country is low, and its pr

Method used

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  • Chlorella pyrenoidosa antioxidant peptide and preparation method thereof
  • Chlorella pyrenoidosa antioxidant peptide and preparation method thereof
  • Chlorella pyrenoidosa antioxidant peptide and preparation method thereof

Examples

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Embodiment 1

[0018] Step 1. Pass 10g of chlorella powder through a 60-mesh sieve to remove impurities, add 100ml of pure water; ultrasonic for 30 minutes, and after 15 minutes (3 / 2s) of treatment with a cell disruptor, freeze-dry to obtain broken-wall chlorella powder and pack it into a bag for later use .

[0019] Step 2. Weigh 10g of broken-wall chlorella powder, add 300ml of pure water, and fully stir to swell, adjust the pH to 5.0, and enzymolysis temperature is 50°C, add 0.1g of cellulase for enzymolysis for 3 hours, and heat up to Inactivate the enzyme at 100°C for 10 minutes, then extract with ultrasonic power 480w for 40 minutes, homogenize for 10 minutes, centrifuge at 8000r / min for 10 minutes, remove the supernatant, precipitate and freeze-dry to obtain Chlorella algae protein.

[0020] Step 3. Weigh 5g of chlorella algae protein, add 100ml of pure water to dissolve, add 0.15g of alkaline protease, adjust the pH to 8.0, enzymolysis temperature 60°C, enzymolysis time 6h, heat up t...

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Abstract

The invention discloses a chlorella pyrenoidosa antioxidant peptide and a preparation method thereof, chlorella pyrenoidosa is used as a raw material, and the chlorella pyrenoidosa antioxidant peptide is obtained through wall breaking treatment of chlorella pyrenoidosa powder, extraction of chlorella protein, preparation of chlorella polypeptide, separation and purification of chlorella polypeptide, and freeze drying. The amino acid sequence of the antioxidant peptide is :VPADDL. The chlorella protein is extracted through adoption of ultrasonic assistance of an enzymolysis method, the salt content is low, and the protein extraction rate is high. Various enzymes are used for preparing the chlorella antioxidative peptide, and the removal rate of hydroxyl radicals is used as a discrimination index, so that the chlorella antioxidative peptide can be more accurately prepared. The algal polypeptide is prepared by adopting the ultrafiltration membrane with the molecular weight cutoff of 3000Da, so that convenient conditions are provided for obtaining purer algal antioxidant peptide through chromatographic separation in the next step. A gel chromatography separation mode is adopted according to different molecular weights of the algae polypeptides, and the purity of the obtained algae polypeptides is higher.

Description

technical field [0001] The invention relates to an antioxidant peptide of chlorella pyrenoidosa and a preparation method thereof. Background technique [0002] Traditional synthetic antioxidants may cause some unexpected side effects. Consumers have a lot of concerns about artificial antioxidants in terms of food safety. Green and healthy foods are increasingly concerned by consumers. Chlorella pyrenoidosa is a single-celled Green microalgae widely distributed in freshwater and seawater. Chlorella has attracted much attention because it is rich in protein, dietary fiber, lipids, vitamins, chlorophyll and carotenoids, as well as active metabolites. In addition, Chlorella also has excellent immune regulation, anti-oxidation, and hypoglycemic and lipid-lowering effects, and has been rated as "green and healthy food" by the Food and Agriculture Organization of the United Nations (FAO). The antioxidant peptide of chlorella pyrenoidosa is a natural antioxidant. At present, the u...

Claims

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Application Information

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IPC IPC(8): C07K14/405C07K1/34C07K1/16C07K1/14C12P21/06
CPCC07K14/405C12P21/06
Inventor 王际辉肖珊王璐王波蔡燕雪
Owner DONGGUAN UNIV OF TECH
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