Marker for detecting triple negative breast cancer as well as detection reagent and application thereof

A triple-negative breast cancer and detection kit technology, applied in the field of tumor detection, can solve the problem of limited drugs for the treatment of triple-negative breast cancer

Active Publication Date: 2021-06-08
SHENZHEN PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, due to the higher metastasis and recurrence rate of triple-negative breast cancer than other breast cancers, long-term use of chemotherapy drugs will lead to patient tolerance, and the current drugs for the treatment of triple-negative breast cancer are very limited

Method used

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  • Marker for detecting triple negative breast cancer as well as detection reagent and application thereof
  • Marker for detecting triple negative breast cancer as well as detection reagent and application thereof
  • Marker for detecting triple negative breast cancer as well as detection reagent and application thereof

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Experimental program
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Effect test

Embodiment 1

[0045] This embodiment provides markers for detecting triple-negative breast cancer. The markers are selected from at least one of ZEB1-AS1, TMEM254-AS1, LINC01087, LINC01122 and LINC00856.

[0046] The primer sequences for detecting the above markers are shown in Table 1.

[0047] Table 1 Primer Sequence

[0048] Primer name Sequence (5'-3') SEQ ID No. ZEB1-AS1_FP GCACAAGTTTGTTCCGCG 6 ZEB1-AS1_RP TCTTTCCGCACTTTTTCCCC 7 TMEM254-AS1_FP ATCCCACAACCATCCACAC 8 TMEM254-AS1_RP TCTCCATACTCTAGTCTCCACC 9 LINC01087_FP CCAACCTCACCCACTCAAAG 10 LINC01087_RP GAGACAAAGAACAGCACTACAAAG 11 LINC01122_FP CATCCTCATTGTACTCCCCTTGG 12 LINC01122_RP GCAATCAGTTTTTCAGCTCCTG 13 LINC00856_FP AGAAGTCCCTGCCAATCAAG 14 LINC00856_RP GGAGGTGGAACAATCTAGAAGTC 15

[0049] Note: FP is an upstream primer, and RP is a downstream primer.

Embodiment 2

[0051] Twelve breast cancer patients were selected, including 3 cases of Luminal A type, 3 cases of Luminal B type, 3 cases of HER2 positive, and 3 cases of TNBC type. All breast cancer patients were from patients with invasive ductal breast cancer diagnosed in the Department of Nail and Breast Surgery, Shenzhen People's Hospital. The tumor tissue is surgically removed, and the adjacent tissue is removed about 3-5cm away from the tumor tissue. The patients ranged in age from 34 to 70 years old.

[0052] experiment procedure

[0053] 1. RNA extraction

[0054] (1) Quickly freeze the tissue in liquid nitrogen, use a grinder to grind the tissue, add 1 mL TRIzol to each tube of tissue, vortex it with a shaker, and shake it slowly on a horizontal shaker for 10 minutes to fully lyse the tissue;

[0055] (2) Add TRIzol-cell lysate to a 1.5mL enzyme-free tube, add 200μL chloroform to each sample, shake vigorously for 15sec, let stand at room temperature for 2-3min, centrifuge at 10...

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Abstract

The invention discloses a marker for detecting triple negative breast cancer as well as a detection reagent and application thereof, and relates to the technical field of tumor detection. By detecting the expression level of at least one marker of ZEB1-AS1, TMEM254-AS1, LINC01087, LINC01122 and LINC00856 and utilizing differentiated expression of the markers in triple negative breast cancer tissue and para-carcinoma tissue of the triple negative breast cancer tissue, the marker can be used for diagnosis, prognosis evaluation or drug screening of the triple negative breast cancer.

Description

technical field [0001] The invention relates to the technical field of tumor detection, in particular to a marker for detection of triple-negative breast cancer, a detection reagent and application thereof. Background technique [0002] Breast cancer is the second leading cause of cancer death among women worldwide. One of the main challenges in its treatment is the heterogeneity of breast cancer cells, which determines the choice of treatment strategies. According to the type of receptor, breast cancer can be divided into 4 subtypes: estrogen / progesterone receptor positive (ER / PR), human epidermal growth factor receptor positive (HER2) and triple negative breast cancer (Triple negative breast cancer). breast cancer, TNBC); and according to the pathological and molecular types, it can be divided into: Luminal A type [Luminal A, (ER / PR positive, HER2 negative)], Luminal B type [Luminal B, (ER / PR positive, HER2 positive)], HER2 positive type and basal type (Basal). Most of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/158C12Q2600/178C12Q2600/118C12Q2600/136Y02A50/30
Inventor 高琳夏金全洪马林邹畅梁真戴勇周文斌赵盼钟晓茹
Owner SHENZHEN PEOPLES HOSPITAL
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