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Microalgae pigment and its extraction method

An extraction method and technology of microalgae, applied in the direction of chemical instruments and methods, organic chemistry, organic dyes, etc., can solve the problems of low purity of microalgae pigments, complicated process routes, and low cost, so as to facilitate industrial production and simplify extraction Process, the effect of reducing the amount of usage

Active Publication Date: 2022-07-15
GUOTOU BIO TECH INVESTMENT CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for extracting microalgae pigments and the microalgae pigments prepared by this method in order to overcome the problems of complex process routes, high cost, and low purity of microalgae pigments in the prior art. The method is simple in process , the obtained pigment has high purity, low energy consumption, low cost and less pollution

Method used

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  • Microalgae pigment and its extraction method
  • Microalgae pigment and its extraction method
  • Microalgae pigment and its extraction method

Examples

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Effect test

Embodiment 1

[0057] This example is used to illustrate the extraction method of fucoxanthin provided by the present invention

[0058] 1) take 500g of diatom powder, use an ultra-fine pulverizer to pulverize the algae powder, and the obtained diatom powder pulverized product has a particle size of 0.5-10 μm;

[0059] 2) in the silica gel chromatography column, add the mixed solvent (n-hexane and ethyl acetate volume ratio is 1:1) that n-hexane and ethyl acetate are formed and use the peristaltic pump to rinse the silica gel column at high speed;

[0060] 3) Add the pulverized algal flour obtained in step 1) into a chromatography column, add a mixed solvent composed of n-hexane and ethyl acetate for elution, and the volume ratio of n-hexane and ethyl acetate is 1:1.

[0061] 4) Collect the eluent containing red components in the chromatographic column, remove the solvent by nitrogen blowing, and then perform vacuum drying to obtain a pigment product; the nitrogen flow rate of the nitrogen b...

Embodiment 2

[0065] This example is used to illustrate the extraction method of fucoxanthin provided by the present invention

[0066] 1) take 500g of diatom powder, use an ultra-fine pulverizer to pulverize the algae powder, and the obtained diatom powder pulverized product has a particle size of 0.5-10 μm;

[0067] 2) Silica gel was charged into the chromatographic column, and the mixed solvent formed by adding n-hexane and ethyl acetate (n-hexane and ethyl acetate volume ratio was 1:0.3) carried out column compression;

[0068] 3) Add the pulverized algal flour obtained in step 1) into a chromatography column, add a mixed solvent composed of n-hexane and ethyl acetate for elution, and the volume ratio of n-hexane and ethyl acetate is 1:0.3.

[0069] 4) Collect the eluent containing red components in the chromatographic column, remove the solvent by nitrogen blowing, and then perform vacuum drying to obtain a pigment product; the nitrogen flow rate of the nitrogen blowing method is 5.0 m...

Embodiment 3

[0072] This example is used to illustrate the extraction method of fucoxanthin provided by the present invention

[0073] 1) take 500g of diatom powder, use an ultra-fine pulverizer to pulverize the algae powder, and the obtained diatom powder pulverized product has a particle size of 0.5-10 μm;

[0074] 2) Silica gel is charged in the chromatography column, and the mixed solvent (n-hexane and ethyl acetate volume ratio is 1:3) formed by adding n-hexane and ethyl acetate is added to carry out column compression;

[0075] 3) Add the pulverized algal flour obtained in step 1) to a chromatography column, add a mixed solvent composed of n-hexane and ethyl acetate for elution, and the volume ratio of n-hexane and ethyl acetate is 1:3.

[0076] 4) Collect the eluent containing red components in the chromatographic column, remove the solvent by nitrogen blowing, and then perform vacuum drying to obtain a pigment product; the nitrogen flow rate of the nitrogen blowing method is 0.5 mL...

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Abstract

The invention relates to the field of preparation of microalgal pigments, and discloses a method for extracting microalgal pigments. The method comprises: pulverizing microalgal biomass to obtain microalgal pulverized products; The algal powder was subjected to column chromatography. The invention also discloses the microalgal pigment prepared by the extraction method of the microalgal pigment. The extraction method of the present invention overcomes the technical prejudice that must first be extracted with an organic solvent and then separated and purified, omits the step of organic solvent extraction, greatly simplifies the extraction process, reduces the usage of organic solvents, and reduces production cost, in favor of industrial production. The method of the present invention can be applied to a variety of microalgae, and the microalgae pigment with higher purity can be obtained through simpler steps.

Description

technical field [0001] The invention relates to the field of preparation of microalgal pigments, in particular to a method for extracting microalgal pigments and the microalgal pigments prepared by the extraction method. Background technique [0002] Microalgae can metabolize and synthesize many high value-added metabolites through the mechanism of photosynthesis, which can be widely used in the fields of food, feed, cosmetics and clothing, mainly including unsaturated fatty acids, proteins, vitamins, pigments, etc. There are many kinds of microalgae pigments, such as β-carotene, phycocyanin, lutein, etc. With the biological activity and antioxidant properties of the pigments synthesized by the metabolism of microalgae have been confirmed, these pigments are used in the fields of food, medicine, cosmetics and textiles. All will have broader application prospects. [0003] In recent years, microalgal pigments have been extracted by pulverization or non-pulverization, organic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D303/32C07D301/32C09B61/00C09B67/54
CPCC07D303/32C07D301/32C09B61/00C09B67/0096
Inventor 喻正保杨小红加晶楚平广韩丹祥胡强
Owner GUOTOU BIO TECH INVESTMENT CO LTD
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