A kind of method for preparing absorption component group of extract of traditional Chinese medicine

A technology of extracts and Chinese medicines, applied in the field of analytical chemistry, can solve the problems of difficult multi-component compound overall preparation, achieve the effect of constant ratio, simple operation process, and no loss of compound amount

Active Publication Date: 2021-12-14
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of this, the present invention provides a method for preparing the absorption component group of the extract of traditional Chinese medicine, which can solve the problem that it is difficult to prepare the multi-component compounds that work together in the extract of traditional Chinese medicine in the prior art

Method used

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  • A kind of method for preparing absorption component group of extract of traditional Chinese medicine
  • A kind of method for preparing absorption component group of extract of traditional Chinese medicine
  • A kind of method for preparing absorption component group of extract of traditional Chinese medicine

Examples

Experimental program
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Effect test

Embodiment 1

[0063] This example is the overall preparation of the orally absorbed components in the Chinese medicine extract.

[0064] (1) Preparation of Chinese medicine extract

[0065] Weigh the standard substance of traditional Chinese medicine compound (Table 1), dissolve it in 75% methanol solution to prepare a stock solution of about 1 mg / mL, and take 200 μL of the above stock solution and mix them to prepare a Chinese medicine compound mixture.

[0066] Table 1 List of traditional Chinese medicine compounds

[0067]

[0068]

[0069] (2) Selection and preparation of liquid / liquid two-phase system I

[0070] According to the empirical value in the prior art, the logP of oral absorption composition 1 =1.8, determine that the liquid / liquid two-phase system I is isopropanol-n-octanol-0.1mol / L phosphate buffer solution ratio is 25:7:25 (v / v / v).

[0071] Separately take isopropanol, n-octanol and 0.1mol / L phosphate buffer solution, add them to the separatory funnel in proportio...

Embodiment 2

[0090] This example is the overall preparation of small intestine absorption components of traditional Chinese medicine compounds.

[0091] (1) Preparation of Chinese medicine extract

[0092] Weigh the standard substance of traditional Chinese medicine compound (Table 3), dissolve it in 75% methanol solution to prepare a stock solution of about 1 mg / mL, and take 200 μL of the above stock solution and mix them to prepare a Chinese medicine compound mixed solution.

[0093] Table 3 list of traditional Chinese medicine compounds

[0094] Chinese name English molecular formula mass-to-charge ratio logP 2

logK 2

3,5-dihydroxybenzoic acid 3,5-Dihydroxybenzoic acid C 7 h 6 o 4

155.0339 0.33 1.07 3,4-Dihydroxybenzoic acid 3,4-Dihydroxybenzoic acid C 7 h 6 o 4

155.0339 0.48 1.16 2,3-Dihydroxybenzoic acid 2,3-Dihydroxybenzoic acid C 7 h 6 o 4

155.0339 -0.09 1.12 2,6-Dihydroxybenzoic acid 2,5-...

Embodiment 3

[0113] This example is the overall preparation of the intestinal absorption component group of traditional Chinese medicine compounds.

[0114] (1) Preparation of Chinese medicine extract

[0115] Weigh the standard substance of traditional Chinese medicine compound (Table 5), dissolve it in 75% methanol solution to prepare a stock solution of about 1 mg / mL, and take 200 μL of the above stock solution and mix it to prepare a Chinese medicine compound mixed solution.

[0116] Table 5 List of traditional Chinese medicine compounds

[0117] Chinese name English name molecular formula mass-to-charge ratio logP 2

Log K 2

cytosine nucleotide Cytidine C 9 h 13 N 3 o 5

244.0928 -2.1 -1.9 adenine nucleotide Adenosine C 10 h 13 N 5 o 4

268.1040 -1.1 -1.3 Guanine nucleotide Guanosine C 10 h 13 N 5 o 5

284.0989 -2.7 -2.0 3,5-dihydroxybenzoic acid 3,5-Dihydroxybenzoic acid C 7 h 6 o 4...

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Abstract

The invention discloses a method for preparing an absorption component group of a traditional Chinese medicine extract, which belongs to the technical field of analytical chemistry and can solve the problem that it is difficult to integrally prepare multi-component compounds in the traditional Chinese medicine extract in the prior art. The method comprises preparing a liquid-liquid two-phase system I; obtaining the distribution coefficient logK of the absorption component group to be prepared in the liquid-liquid two-phase system I 1 Range value; according to partition coefficient logK 1 The range value and the retention value of the stationary phase of the liquid-liquid two-phase system I in the high-speed countercurrent chromatograph were used to obtain the collection time of the absorption component group to be prepared; using high-speed countercurrent chromatography, according to the collection time of the absorption component group to be prepared, the extraction of traditional Chinese medicine was prepared. Groups of absorbed components in substances. The method of the present invention obtains the distribution coefficient logK of the absorption component group to be prepared in the liquid-liquid two-phase system I 1 Range value, the compounds in the Chinese medicine extract were divided into absorbing component group and non-absorbing component group, and the absorbing component group of the Chinese medicine extract was prepared by high-efficiency countercurrent chromatography.

Description

technical field [0001] The invention relates to the technical field of analytical chemistry, in particular to a method for preparing an absorption component group of a traditional Chinese medicine extract. Background technique [0002] Traditional Chinese medicine, especially compound prescriptions, has a complex composition of compounds. According to the current knowledge, it is impossible for all of these components to work, but we still don't fully know which components work. [0003] At present, most of the technologies widely used in the separation and preparation of traditional Chinese medicine are derived from the column chromatography technology of western medicine, such as C 18 column, gel column, ion exchange column, macroporous resin, polyamide column, etc. Column chromatography technology is a technology to separate compounds according to the different adsorption capacities of compounds on fillers. However, compounds in Chinese medicine extracts have complex con...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01D15/18A61K36/236
CPCB01D15/1807A61K36/236A61K2236/55
Inventor 张晓哲刘丹刘欣欣程孟春赵凡赵楠
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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