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Verification method of microwave-assisted biological enzyme catalytic reaction

A microwave-assisted, catalytic reaction technology, applied in the field of biological enzymes

Active Publication Date: 2021-06-18
HARBIN UNIV OF COMMERCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no research result yet to show exactly what effect causes microwave radiation to change the result of the enzymatic chemical reaction. For this point, there are two different views in the academic circles, one is "thermal effect", and the other is related to temperature. Irrelevant "non-thermal effects"

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  • Verification method of microwave-assisted biological enzyme catalytic reaction
  • Verification method of microwave-assisted biological enzyme catalytic reaction
  • Verification method of microwave-assisted biological enzyme catalytic reaction

Examples

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Embodiment 1

[0028] This example provides a method for verifying microwave-assisted biological enzyme-catalyzed reactions, which is used to reveal the role of microwave radiation in enzymatically-catalyzed reactions, and to determine the principle of microwave radiation improving enzyme-catalyzed reactions.

[0029] The verification method of the microwave-assisted biological enzyme-catalyzed reaction provided in this embodiment includes the following steps:

[0030] S1. Mix cornstarch with different concentrations of chloroauric acid heated to boiling according to the mass ratio of 1:0.06. The mass concentration of the chloroauric acid is 0.001%-0.01%, and react for 15s to prepare starch-gold particle composite The average particle size of the prepared gold particles is 1nm-10μm.

[0031] S2. Add Candida antarctica lipase to the chloroauric acid solution with a mass concentration of 0.01%, react in a constant temperature oscillator at 35° C. for 36 hours, wherein the mass ratio of chloroa...

Embodiment 2

[0036] This embodiment provides a verification method for a microwave-assisted biological enzyme-catalyzed reaction, which includes the following steps:

[0037] S1. Mix cornstarch with different concentrations of chloroauric acid heated to boiling according to the mass ratio of 1:0.3, the mass concentration of the chloroauric acid is 0.001%-0.01%, and react for 15s to prepare starch-gold particle composite The average particle size of the prepared gold particles is 1nm-10μm.

[0038] S2. Add Candida antarctica lipase to the chloroauric acid solution with a mass concentration of 0.01%, react in a constant temperature oscillator at 35° C. for 36 hours, wherein the mass ratio of chloroauric acid to Candida antarctica lipase is 1 : 4.5, lipase-gold particle hybrids are obtained, and in the hybrids, the average particle diameter of the gold particles is 1nm-10 μm.

[0039] S3. Mix the starch-gold particle complex obtained in step S1 with oleic acid, and add the lipase-gold partic...

Embodiment 3

[0043] This embodiment provides a verification method for a microwave-assisted biological enzyme-catalyzed reaction, which includes the following steps:

[0044] S1. Mix the activated cornstarch with different concentrations of auric acid chloride heated to boiling according to the mass ratio of 1:0.15, the mass concentration of the auric acid chloride is 0.001%-0.01%, and react for 15s to prepare starch- The gold particle composite, the average particle size of the prepared gold particles is 1nm-10μm.

[0045]S2. Add Candida antarctica lipase to the chloroauric acid solution with a mass concentration of 0.01%, react in a constant temperature oscillator at 35° C. for 36 hours, wherein the mass ratio of chloroauric acid to Candida antarctica lipase is 1 : 4, lipase-gold particle hybrids are obtained, and in the hybrids, the average particle diameter of the gold particles is 1nm-10 μm.

[0046] S3. Mix the starch-gold particle complex obtained in step S1 with oleic acid, and ad...

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Abstract

The invention discloses a verification method of a microwave-assisted biological enzyme catalytic reaction. The method comprises the following steps: with adoption of different particle sizes of nanogold, a starch-nanogold compound and a lipase-nanogold hybrid are taken as microwave absorbers or transmitters, the lipase catalytic reaction is enhanced by microwave radiation through a thermal effect and a non-thermal effect respectively, and the influence of the microwave thermal effect and non-thermal effect on enhancing the enzymatic reaction is verified. Finally, the enhancement effect relationship between the non-thermal effect and the thermal effect of microwave radiation and the substrate structure and the influence on the enzymatic reaction are disclosed, so that the mechanism of nanogold-assisted microwave enhanced lipase catalytic reaction is clarified, and the technical effect of accelerating the industrialization of microwave enhanced enzyme catalytic synthesis is achieved.

Description

technical field [0001] The invention belongs to the technical field of biological enzymes, and in particular relates to a microwave-assisted verification method for catalytic reactions of biological enzymes. Background technique [0002] Enzyme catalysis is a powerful catalytic method in modern organic synthetic chemistry. Microwave technology has also been successfully applied to strengthen various organic chemical reactions. Microwave-assisted strengthening of organic synthesis reactions can not only greatly increase the reaction rate and yield, but also Energy consumption is saved to a certain extent. With the deepening of chemical research and the cross-development of disciplines, the combination of microwave enhancement technology and enzyme catalysis technology to promote organic reactions will produce effects that cannot be observed when applied alone. Since the 1990s, researchers have applied microwave radiation to organic phase enzyme-catalyzed reactions, and found...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12Q1/44G01N21/35
CPCC12P19/04C12Q1/44G01N21/35G01N2333/92
Inventor 王艳赵宁辛嘉英孙立瑞王悦李虹佳
Owner HARBIN UNIV OF COMMERCE
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