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Primer and kit for detecting peripheral blood circulation microRNA related to lung cancer diagnosis and treatment

A technology for peripheral blood circulation and lung cancer, applied in the fields of biotechnology and diagnosis, can solve problems such as risks, difficulty in obtaining tissue samples, and restrictions on the application of molecular targeted therapy, and achieve high accuracy and sensitivity

Inactive Publication Date: 2021-06-18
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, lung puncture causes great trauma to the patient, and there are risks
Especially for patients with middle and advanced tumors who cannot be operated on, the application of molecular targeted therapy is severely limited due to the difficulty in obtaining tissue samples

Method used

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  • Primer and kit for detecting peripheral blood circulation microRNA related to lung cancer diagnosis and treatment
  • Primer and kit for detecting peripheral blood circulation microRNA related to lung cancer diagnosis and treatment
  • Primer and kit for detecting peripheral blood circulation microRNA related to lung cancer diagnosis and treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: MicroRNA molecules up-regulated in peripheral blood to assist in the diagnosis of lung cancer:

[0020] The present invention provides 12 kinds of circulating microRNA molecules with up-regulated expression in peripheral blood that assist in the diagnosis of lung cancer. Such biomolecules can be used alone or in combination of two or more to diagnose lung cancer, specifically including hsa-miR-16-5p, hsa-miR-26a -5p, hsa-miR-15b-5p, hsa-miR-21-5p, hsa-miR-191-5p, hsa-miR-195-5p, hsa-miR-223-3p, hsa-miR-92a-3p , hsa-let-7a-5p, hsa-let-7f-5p, hsa-miR-451a and hsa-miR-150-5p, the corresponding nucleotide sequences are shown in Table 1.

[0021] Supplementary Table 1 Circulating microRNA molecules with up-regulated expression in peripheral blood to aid in the diagnosis of lung cancer

[0022]

[0023]

[0024] The reverse transcription primer (Universal RT primer) sequence is:

[0025] 5'-GGATCGCCCTTGCTGTCAACGATACGCTACGTAACGGCATGACAGTG(T)n-3'

[0026] Th...

Embodiment 2

[0031] Example 2: Screening and identification of diagnostic markers for lung cancer

[0032] In the present invention, the real-time fluorescent quantitative PCR method was used to screen and identify circulating microRNA markers in peripheral blood samples collected from 30 patients with pathologically diagnosed non-small cell lung cancer. The peripheral blood samples of all lung cancer patients were provided by Dalian Central Hospital, and the blood samples of healthy people were provided by Dalian Physical Examination Center. The subjects have been informed of their consent and signed an informed consent form.

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Abstract

The invention discloses a group of peripheral blood circulation microRNAs related to diagnosis and treatment of lung cancer and application of the peripheral blood circulation microRNAs. The microRNA is prepared from the following components: hsa-miR-16-5p, hsa-miR-26a-5p, hsa-miR-15b-5p, hsa-miR-21-5p, hsa-miR-191-5p, hsa-miR-195-5p, hsa-miR-223-3p, hsa-miR-92a-3p, hsa-let-7a-5p, hsa-let-7f-5p, hsa-miR-451a and hsa-miR-150-5p. The microRNA specific detection primer and the application of the microRNA specific detection primer in the kit are firstly protected; and a product has a function of diagnosis or auxiliary diagnosis of lung cancer. In addition to protecting a specific primer sequence, a reverse transcription primer, a specific upstream primer and a universal downstream primer are further included; and the microRNA is obviously up-regulated in peripheral blood of the lung cancer, has the advantages of good stability, high specificity and high sensitivity, can be independently used or a combination of more than two microRNAs to assist clinical early diagnosis or prognosis monitoring of the lung cancer, and has important application value.

Description

technical field [0001] The invention belongs to the field of biotechnology and diagnosis, and in particular relates to a method for assisting the diagnosis and treatment of lung cancer by microRNA circulating in peripheral blood and its application. Background technique [0002] Lung cancer is currently one of the malignant tumors with the highest morbidity and mortality in the world, of which 80% to 85% are non-small cell lung cancer (NSCLC), and the 5-year survival rate is less than 15%. According to the 2014 World Cancer Report, the incidence of lung cancer in China has exceeded 1 / 3 of the global proportion. Molecular targeted therapy is considered to be the most specific individualized treatment for lung cancer. Compared with traditional treatments, molecular targeted therapy has the characteristics of low cytotoxicity and high specificity, and selectively transports drugs or inhibitors to specific target cells, tissues or organs through specific carriers to inhibit tum...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/178C12Q2600/118C12Q2600/158
Inventor 朴海龙刘秀梅
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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