LAMP primer group for detecting giardia, kit and application

Abstract

The invention provides an LAMP (loop-mediated isothermal amplification) primer group for detecting giardia, a kit and application, and relates to the technical field of biological diagnosis and detection. The LAMP primer group disclosed by the invention is designed by taking a giardia triosephosphate isomerase (TPI) gene as a target gene. According to the primer group or the kit, amplification of giardia nucleic acid can be completed within 30 min based on the LAMP micro-fluidic chip technology, the detection sensitivity reaches 10 copies / microliter and 0.01 ng nucleic acid / microliter, and the primer group or the kit is good in giardia specificity, high in repeatability and good in stability.

Description

technical field [0001] The invention belongs to the technical field of biological diagnosis and detection, and in particular relates to a LAMP primer set, a kit and an application for detecting Giardia. Background technique [0002] Giardia is a very common pathogenic zoonotic pathogen, and it is also the main pathogen that causes infectious diarrhea. It can be transmitted through drinking water, food, contact, etc., causing Giardia disease outbreak. The U.S. Foodborne Disease Surveillance Organization classifies Giardia as one of the top ten parasites for foodborne disease surveillance. The average infection rate of Giardia in the world is 4% to 5%, and it is called "the number one intestinal parasite" in countries with severe infection. [0003] In view of the occurrence of water-borne and food-borne incidents of Giardia, the detection of Giardia contamination in environmental media has become a key link in the control of infectious diarrhea outbreaks. At present, the m...

AI Technical Summary

Problems solved by technology

Etiological detection methods include wet slides or microscopic examination after staining. Among them, the modified acid-fast staining method and iodine staining method are considered to be the "gold standard" for the diagnosis of Giardia, but this method requires the detection personnel to have certain practical skills. Experience has led to certain missed and false detections in this method; molecular biology detection methods mainly include PCR, RPA, LAMP and other technologies, the advantages of which are reliable detection results, can handle high-throughput samples, and can further carry out species identification, Genotyping, evolutionary tree analysis, etc.; the disadvantage is that the process steps are cumbersome, time-consuming and labor-intensive, and there are many problems caused by objective factors; immunological detection methods include fluorescent-labeled antibody detection, enzyme-labeled antibody detection and other methods based on the specific combination of antigen and antibody Principle, needs to extract serum or tissue, but the operation is slightly difficult

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