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Animal enzyme for saline-alkali soil improvement and preparation method thereof

A saline-alkali land and animal technology, applied in the field of bioengineering, can solve problems such as hindering the improvement of soil fertility, low product added value, land use hazards, etc., and achieve the effects of reducing pH value, low product added value, and high treatment costs.

Inactive Publication Date: 2021-06-29
SHANDONG BAIDE BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The problem of soil salinization is a worldwide problem, which has caused great harm to my country's land use and seriously hindered the improvement of land fertility.
[0003] And our country is a large animal husbandry country. The domestic dead animals are more than 4 million tons every year. These animal carcasses are generally treated by traditional methods such as landfill, incineration, and high-temperature system. However, these processing methods have environmental pollution, high processing costs, and The added value of the product is low, and these resources such as protein and fat cannot be fully utilized. Therefore, it is also necessary to find a new treatment method to expand the application range of these materials

Method used

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  • Animal enzyme for saline-alkali soil improvement and preparation method thereof
  • Animal enzyme for saline-alkali soil improvement and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043]Put 1000 kg of sick and dead chickens into the crushing device for crushing, and crush the material to below 20 mesh. Then the material is sent to the crushing device for crushing treatment, and the crushing is below 100 mesh. The crushed material is sterilized at 80° C. for 1 hour. Stir continuously during the sterilization process, and the stirring frequency is 5r / min. Add 2% mass of glucose to the sterilized material, and cool down to 55°C. After cooling down, add 0.3% compound enzyme preparation (0.4 part of acid protease and 0.6 part of lipase), stir the enzymolysis for 3 hours, the stirring frequency is 5 r / min, and adjust the pH value in the range of 6.0-7.0. The slurry prepared in the step is transported to an aerobic fermentation container, inoculated with 0.1% fermentation bacteria A agent (0.6 parts of Bacillus subtilis, 0.6 parts of Bacillus licheniformis and 0.8 parts of Saccharomyces cerevisiae), stirring frequency 5r / min, and feeding Air 0.4VVm, ferment...

Embodiment 2

[0047] Put 1000kg of sick and dead pigs into the crushing device for crushing treatment, and crush the material to below 20 mesh. Then the material is sent to the crushing device for crushing treatment, and the crushing is below 100 mesh. The crushed material is sterilized at 80° C. for 1 hour. Stir continuously during the sterilization process, and the stirring frequency is 5r / min. Add 2% mass of glucose to the sterilized material, and cool down to 55°C. After cooling down, add 0.3% compound enzyme preparation (0.4 part of acid protease and 0.6 part of lipase), stir the enzymolysis for 3 hours, the stirring frequency is 5 r / min, and adjust the pH value in the range of 6.0-7.0. The slurry prepared in the step is transported to an aerobic fermentation container, inoculated with 0.1% fermentation bacteria A agent (0.6 parts of Bacillus subtilis, 0.6 parts of Bacillus licheniformis and 0.8 parts of Saccharomyces cerevisiae), stirring frequency 5r / min, and feeding Air 0.4VVm, f...

experiment example 1

[0051] The animal enzyme used for saline-alkali soil improvement prepared in Example 1 was sealed and packaged in 12 barrels in plastic barrels, and two barrels were opened for sampling and testing every two months to detect pH, Salmonella, Escherichia coli, and Staphylococcus aureus, and the test results took two barrels The average value of the results can be seen in Table 3.

[0052] Table 3 is used for the preservation test data of the animal enzyme of saline-alkali land improvement

[0053] time February April June August October December pH value 4.8 4.8 4.7 4.6 4.6 4.6 Salmonella (per 25ml) not detected not detected not detected not detected not detected not detected Escherichia coli (cfu / ml) ＜10 ＜10 ＜10 ＜10 ＜10 ＜10 Staphylococcus aureus (cfu / ml) 0 0 0 0 0 0

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Abstract

The invention discloses an animal enzyme for saline-alkali soil improvement. The animal enzyme is prepared from the following components in parts by weight: 60 to 100 parts of an animal protein raw material, 0.1 to 0.5 part of a compound enzyme preparation, 0.1 to 0.5 part of a fermentation inoculant A, 0.1 to 1 part of a fermentation inoculant B and 1 to 2 parts of auxiliary materials. Meanwhile, the invention discloses a preparation method and application of the animal enzyme for saline-alkali soil improvement. The production process is simple and reasonable, the raw materials are easy to obtain, the produced product is remarkable in functionality and good in saline-alkali soil improvement effect, and the utilization field of breeding waste is widened.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, in particular to an animal enzyme for improving saline-alkali land and a preparation method thereof. Background technique [0002] The problem of soil salinization is a worldwide problem, which has caused great harm to my country's land use and seriously hindered the improvement of land fertility. In addition, due to incomplete saline-alkali land treatment measures and incomplete treatment technology, the area of ​​saline-alkali land shows an increasing trend year by year. Because the saline-alkali land is not suitable for crop cultivation, a large amount of arable land is idle, and it cannot be used in other ways through short-term transformation, which poses a huge threat to my country's food security and rational use of land resources. The distribution of saline-alkali soil in my country is relatively wide, mainly in the northeast, northwest, north and coastal areas of China, with a to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K17/14C09K17/32C09K101/00C09K109/00
CPCC09K17/14C09K17/32C09K2101/00C09K2109/00
Inventor 季维峰何永聚印遇龙李东廖鹏康术美张宏灿李峰陈湘江陈振勇
Owner SHANDONG BAIDE BIOLOGICAL TECH
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