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Hemerocallis fulva waterlogging tolerance identification method

An identification method and technology of waterlogging tolerance, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of rarely reported waterlogging tolerance of daylilies

Pending Publication Date: 2021-07-06
SHANGHAI INST OF TECH
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Problems solved by technology

[0004] The identification of waterlogging tolerance of Hemerocallis is the basis for breeding fine varieties of Hemerocallis. Many scholars have studied the resistance of Hemerocallis, but there are few reports on the waterlogging tolerance of Hemerocallis

Method used

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  • Hemerocallis fulva waterlogging tolerance identification method
  • Hemerocallis fulva waterlogging tolerance identification method
  • Hemerocallis fulva waterlogging tolerance identification method

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Embodiment Construction

[0048] The present invention will be further described below in conjunction with specific embodiments and accompanying drawings, so as to help understand the content of the present invention.

[0049] Such as Figure 1-7 Shown, a kind of Hemerocallis waterlogging tolerance identification method provided by the present invention comprises the following steps:

[0050] S1: Flooding treatment: Put the gallon basin into a large plastic box, flood the soil surface 5-10cm, replenish water regularly to ensure that the water level remains unchanged, and continue flooding for 8 days. The leaves of the treated plants were taken on the 0d, 2d, 4d, 6d and 8d of the flooding, and 0.1g leaves were taken from each plant, and repeated 3 times. The leaves were removed and placed in liquid nitrogen, and then transferred to a -70°C refrigerator for storage.

[0051] S2: Determination and analysis of physiological and biochemical indicators

[0052] ①Determination of leaf antioxidant enzyme act...

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Abstract

The invention discloses a hemerocallis fulva waterlogging tolerance identification method, which aims at screening hemerocallis fulva waterlogging tolerance resources, selects different varieties of hemerocallis fulva to carry out waterlogging stress, takes leaves as samples, and carries out principal component analysis on seven physiological and biochemical indexes to obtain two independent indexes, and through comprehensive analysis, the day lily varieties are divided into four types: waterlogging-resistant variety, waterlogging-resistant variety, non-waterlogging-resistant variety and non-waterlogging-resistant variety. According to the method, evaluation is carried out through multi-index measurement and comprehensive data results, the problem that the waterlogging tolerance identification process of hemerocallis fulva is not comprehensive enough is solved, and the evaluation result is objective and repeatable. The method provided by the invention has the remarkable characteristics of no damage, high efficiency, low cost and intuition.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation and breeding, and in particular relates to a method for identifying waterlogging tolerance of daylilies. Background technique [0002] Hemerocallis belongs to the perennial root herb of Liliaceae (Liliaceae). It is native to Asian and European countries. There are about 14 species of Hemerocallis in the world, and 11 species are native to my country. Its germplasm resources are abundant and its functions are extensive. "View as a flower, eat as a vegetable, and use as a medicine", the market demand for it continues to increase (Chen Lifei, Dong Ran. "Research Progress in Hemerocallis". Changchun: Department of Landscape Architecture, College of Horticulture, Jilin Agricultural University, 2007, 1), in Ornamental plants have the advantages of long viewing period, high ornamental value and strong adaptability. They are excellent garden ornamental flowers and are widely used in urban landscap...

Claims

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Application Information

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IPC IPC(8): G01N33/00
CPCG01N33/0098
Inventor 尹冬梅齐帅赵安琪尹高菲高腾尹文昕尹天宸高馨远王婧董婷婷高巍王月悦陈柯霖
Owner SHANGHAI INST OF TECH
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